ID A0A2B8ALB4_9ACTN Unreviewed; 1435 AA.
AC A0A2B8ALB4;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=CRI70_15980 {ECO:0000313|EMBL:PGH49664.1};
OS Streptomyces sp. Ru87.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2044307 {ECO:0000313|EMBL:PGH49664.1, ECO:0000313|Proteomes:UP000222409};
RN [1] {ECO:0000313|EMBL:PGH49664.1, ECO:0000313|Proteomes:UP000222409}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ru87 {ECO:0000313|EMBL:PGH49664.1,
RC ECO:0000313|Proteomes:UP000222409};
RA Amin D.H., Borsetto C., Abolmaaty A., Tolba S., Abdallah N.A.,
RA Wellington E.M.H.;
RT "Draft genome sequence of Streptomyces sp. Ru87 isolated from Egyptian
RT soil.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PGH49664.1}.
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DR EMBL; PDIX01000260; PGH49664.1; -; Genomic_DNA.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000222409; Unassembled WGS sequence.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR046816; MmeI_Mtase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; NF033455; BREX_6_MTaseX; 1.
DR PANTHER; PTHR33841:SF1; ADENINE-SPECIFIC METHYLTRANSFERASE PGLX; 1.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR Pfam; PF20473; MmeI_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:PGH49664.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000222409};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:PGH49664.1}.
FT DOMAIN 331..419
FT /note="MmeI-like DNA-methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF20473"
FT REGION 676..695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1125..1152
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1435 AA; 160956 MW; D64379DAAB4A096F CRC64;
MPMTSDAKRA LSSTIRSLRA RLLEDLHSAT EMAYRLSVYA RDAGLDEAAG ARRNRLEAWV
AEQLRAQGAA KAKGGDRSAD DFRREAEKQA AYTLLNRLVM LRLMEAPGPS GQPLRAPTVV
TEGWESRGYK DLRQLAPGLV RGDETEGYAF LLRLIFEDLA TELPGLYGPA GVADLVPIPA
ATLRHVVDAL NADALESCWT DDMTLGWVYQ YWNDPERELL DDKLNGGGKV EPHEIASKTQ
MFTERYMVDW LLQNSLGPMW LATCRKHGWT PEVQADGTFD RLEKRRTEWR AKRASREVAL
TDLMPLHTDA ERRWAYYVPQ PIPEDAVEQA PETVRELKLL DPAVGSGHFL VVALDLLVAL
YREEARHRGE AGEERWSDRA IVERILSHNL HGIDLDPRAA QIAAAALWLK AQQVAPDARP
ERLNLVASNL RLAGLADDDP ALVELRREVE RETGIPGTLT DTLIHALRGA DHLGSLLKVD
TAVDDAIERH EAVLGRAVTE QGDLFADYKP QPERVAIGRD EAKATLLDLF EGFLAKHTGG
DDLGLRLRGE QLAAGVRFVR LVRQGAYDLV VANPPYQGTS KMADTKYIEK TYALGKADLY
AAFLLRGLEL VRDGGVSAML TMRNWMFIKQ YAGLREHVLR NFDVRALGDF AVGAFDEVPN
DLLSVTVSVL HRSTQRPELS VAQQPTPPDD MSYDRVRTRR KRAATLCHEG RHAFDPAALK
VVPEWPLVYW WGTEFLVHYA GAPKLGEVSP ARSGAQTSND TRFLRRSWEV NQSAIEIVAR
WKQRATPRRW APYVTGAKGL RWIDPLSTIV EWDSAGLIVR VFNEHLYGAH SRTVKNENLY
WKPGIAFSTI GNDFGARLHH SRAIFGVKGA SVFPESEDSC FRVLCLLNSS LARQTVGSLN
PGIDFTSGDI NRLPVVAIAD AEVIGETLVG KFAEHEAARE TSHAFRRPGP SPWRHAQDWA
QAAVDRPEGA QLPEYVGQLD PEPSTDHLSF ALGVALGRFS PAESAAEGIL DPATADLSHA
LPHGMLFLDT TLDAEDRRDG LGHPAAVPLH AAWAQHGLAN GTKRSLRDWL ARDFFKDVHK
GMYENRPIHW PLSSPDKTFV AWVNIHRMEE QSMRVLLADY LVPTLARLDG ELSDLRSARE
SANKKASRAA EQQYDRVLKA RRELQVFIAD VERCADKGAP PTDNKCLPRE QDARYAPSLD
DGVMINSAAL WPLLDPQWKD PRKWWKELST AQGRKDYDWS HLAMRYWPTR VDRKCRQDPS
LAVTHGCFWR YHPGRAWCWE LRLQDEIGPG FKIEEAPSQL GGRDRGDDGD RLHRDAFLRD
HPTKALAAIE KEAVRRMGRG KSRKLVPEMR IFETGLWSAH AEALWEMELR LAEKQGAELH
ILAPDEPEAR AAFESQYPQK VRARKDFLAD LVPPVELFEN EDAYDDAVTT EENNT
//
