ID A0A2C5X1S2_9PEZI Unreviewed; 813 AA.
AC A0A2C5X1S2;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=cysteine--tRNA ligase {ECO:0000256|ARBA:ARBA00012832};
DE EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN Name=YNL247W {ECO:0000313|EMBL:PHH51920.1};
GN ORFNames=CFIMG_005819RAa {ECO:0000313|EMBL:PHH51920.1};
OS Ceratocystis fimbriata CBS 114723.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Microascales; Ceratocystidaceae; Ceratocystis.
OX NCBI_TaxID=1035309 {ECO:0000313|EMBL:PHH51920.1, ECO:0000313|Proteomes:UP000222788};
RN [1] {ECO:0000313|EMBL:PHH51920.1, ECO:0000313|Proteomes:UP000222788}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 114723 {ECO:0000313|EMBL:PHH51920.1,
RC ECO:0000313|Proteomes:UP000222788};
RX PubMed=23931120; DOI=10.1016/j.funbio.2013.06.004;
RA Simpson M.C., Wilken P.M., Coetzee M.P., Wingfield M.J., Wingfield B.D.;
RT "Analysis of microsatellite markers in the genome of the plant pathogen
RT Ceratocystis fimbriata.";
RL Fungal Biol. 117:545-555(2013).
RN [2] {ECO:0000313|EMBL:PHH51920.1, ECO:0000313|Proteomes:UP000222788}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 114723 {ECO:0000313|EMBL:PHH51920.1,
RC ECO:0000313|Proteomes:UP000222788};
RX PubMed=24563841; DOI=10.5598/imafungus.2013.04.02.14;
RA Wilken P.M., Steenkamp E.T., Wingfield M.J., de Beer Z.W., Wingfield B.D.;
RT "IMA Genome-F 1: Ceratocystis fimbriata: Draft nuclear genome sequence for
RT the plant pathogen, Ceratocystis fimbriata.";
RL IMA Fungus 4:357-358(2013).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PHH51920.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; APWK03000082; PHH51920.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2C5X1S2; -.
DR STRING; 1035309.A0A2C5X1S2; -.
DR OrthoDB; 2140072at2759; -.
DR Proteomes; UP000222788; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00672; CysRS_core; 1.
DR Gene3D; 1.20.120.1910; Cysteine-tRNA ligase, C-terminal anti-codon recognition domain; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR015273; Cys-tRNA-synt_Ia_DALR.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00435; cysS; 1.
DR PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR Pfam; PF09190; DALR_2; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR PRINTS; PR00983; TRNASYNTHCYS.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:PHH51920.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000222788};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 48..486
FT /note="tRNA synthetases class I catalytic"
FT /evidence="ECO:0000259|Pfam:PF01406"
FT DOMAIN 538..587
FT /note="Cysteinyl-tRNA synthetase class Ia DALR"
FT /evidence="ECO:0000259|Pfam:PF09190"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 777..797
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 715..755
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 813 AA; 91246 MW; C2C9C82992106130 CRC64;
MASTSAARQQ PPWTPPKPLA AGIKIPDLKV LNSLTRKKED FVPLDSAGKK VSWYTCGPTV
YDDAHLGHAR NYVSTDIIRR IMKDYFNFNV QFVMNITDVD DKIILRGRQQ YLLDNMKTEH
PTIDEFVKTT TKTAFEKYIL KNLPLLPADV TPGTYDAQAA AAYKRVLDGL ALDSDTESAG
DKEAKLKMHI KTVATAAEAL REASASSLDP ATFYDKADNV LLPYLDSLHG AGIDANDHSI
FTKLTQKFET RFFEDMAALN VLYPDVLTRV SEYVPQIVSF IERIVKNGYG YATEDGSVYF
DIAAFEKDGH HYACLEPWNR NDKSLLADGE GALSSKATTV KRNECDFALW KSSKAGEPSW
KSPWGLGRPG WHIECSVMAS EVLGEAMDFH SGGVDLRFPH HDNELAQSTA YWTSEHPNVQ
WTNYFLHTGH LSIQGMKMSK SLKNFTTIRE ALAREEWTQR SLRICFLMGT WQDGIEITEE
LMKSTTSWED KLNNFFLKAM DVERNPKTTE AAAAAIGSPE TDKKLLADLE KAKTDLHTAL
CDSFNTPVAM RVISDLVSEF NTAKCASTET TQEIARWITR IVTIFGLDSK NDLSDHKRVG
WSGIDIPNEA RSFIYPTAEL RDEVRQQARS GTLDHGSIAR LVDAVNAPPA SGAEEKKYEG
VLEQFKTDVK KLAAEKAAAK DLLMLCDQLR DTHLWNLGIY LEDRDAPLKA MVRPVDKALV
AAREEREEIA AAKAAAKAKR EAEAAEKARL LEEKAKLSHL DMFKTAEYAE WDAEGLPTKD
AKGEEIPKSK KKKLMKEWEK QKKLHEEWLA KQK
//
