ID   A0A2C6LH03_9FIRM        Unreviewed;       152 AA.
AC   A0A2C6LH03;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   03-MAY-2023, entry version 16.
DE   SubName: Full=HIT family hydrolase {ECO:0000313|EMBL:PHJ37520.1};
GN   ORFNames=P378_15690 {ECO:0000313|EMBL:PHJ37520.1};
OS   Desulforamulus profundi.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Peptococcaceae;
OC   Desulforamulus.
OX   NCBI_TaxID=1383067 {ECO:0000313|EMBL:PHJ37520.1, ECO:0000313|Proteomes:UP000222564};
RN   [1] {ECO:0000313|EMBL:PHJ37520.1, ECO:0000313|Proteomes:UP000222564}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bs107 {ECO:0000313|EMBL:PHJ37520.1,
RC   ECO:0000313|Proteomes:UP000222564};
RA   Aullo T., Berlendis S., Lascourreges J.-F., Dessort D., Saint-Laurent S.,
RA   Schraauwers B., Mas J., Magot M., Ranchou-Peyruse A.;
RT   "Biodegradation of hydrocarbons in the deep terrestrial subsurface :
RT   characterization of a microbial consortium composed of two Desulfotomaculum
RT   species originating from a deep geological formation.";
RL   Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PHJ37520.1}.
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DR   EMBL; AWQQ01000090; PHJ37520.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2C6LH03; -.
DR   Proteomes; UP000222564; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   CDD; cd01275; FHIT; 1.
DR   Gene3D; 3.30.428.10; HIT-like; 1.
DR   InterPro; IPR039383; FHIT.
DR   InterPro; IPR011146; HIT-like.
DR   InterPro; IPR036265; HIT-like_sf.
DR   PANTHER; PTHR42997:SF1; AP-4-A PHOSPHORYLASE; 1.
DR   PANTHER; PTHR42997; HIT FAMILY HYDROLASE; 1.
DR   Pfam; PF01230; HIT; 1.
DR   SUPFAM; SSF54197; HIT-like; 1.
DR   PROSITE; PS51084; HIT_2; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000313|EMBL:PHJ37520.1};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000222564}.
FT   DOMAIN          21..133
FT                   /note="HIT"
FT                   /evidence="ECO:0000259|PROSITE:PS51084"
FT   MOTIF           117..121
FT                   /note="Histidine triad motif"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00464"
FT   ACT_SITE        119
FT                   /note="Tele-AMP-histidine intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-1"
FT   BINDING         48
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-2"
FT   BINDING         121
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-2"
SQ   SEQUENCE   152 AA;  17271 MW;  2A82A4B13E494856 CRC64;
     MENLWAPWRS IYIGKPQTGC IFCEKLNSDE DKENLVVYRG DKTFVIMNLY PYNNGHLLIA
     PKRHVGDITE LTEEELLELN KMTRFMVQSL RRAFGNPHGF NIGMNLGKVA GAGIPGHLHV
     HIVPRWKVTR ILWQSSGIPG SFPRGCKGPM KK
//