ID   A0A2C9JRV7_BIOGL        Unreviewed;       462 AA.
AC   A0A2C9JRV7;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   RecName: Full=tRNA wybutosine-synthesizing protein 2 homolog {ECO:0000256|ARBA:ARBA00017179};
DE            EC=2.5.1.114 {ECO:0000256|ARBA:ARBA00012265};
DE   AltName: Full=tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00031315};
GN   Name=106065493 {ECO:0000313|EnsemblMetazoa:BGLB007049-PB};
OS   Biomphalaria glabrata (Bloodfluke planorb) (Freshwater snail).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Panpulmonata; Hygrophila; Lymnaeoidea;
OC   Planorbidae; Biomphalaria.
OX   NCBI_TaxID=6526 {ECO:0000313|EnsemblMetazoa:BGLB007049-PB, ECO:0000313|Proteomes:UP000076420};
RN   [1] {ECO:0000313|EnsemblMetazoa:BGLB007049-PB}
RP   IDENTIFICATION.
RC   STRAIN=BB02 {ECO:0000313|EnsemblMetazoa:BGLB007049-PB};
RG   EnsemblMetazoa;
RL   Submitted (MAY-2020) to UniProtKB.
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent transferase that acts as a
CC       component of the wybutosine biosynthesis pathway. Wybutosine is a hyper
CC       modified guanosine with a tricyclic base found at the 3'-position
CC       adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes
CC       the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-
CC       adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14)
CC       to produce wybutosine-86. {ECO:0000256|ARBA:ARBA00037786}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine =
CC         4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) +
CC         H(+) + S-methyl-5'-thioadenosine; Xref=Rhea:RHEA:36355, Rhea:RHEA-
CC         COMP:10164, Rhea:RHEA-COMP:10378, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:64315,
CC         ChEBI:CHEBI:73550; EC=2.5.1.114;
CC         Evidence={ECO:0000256|ARBA:ARBA00036405};
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DR   RefSeq; XP_013079783.1; XM_013224329.1.
DR   RefSeq; XP_013079784.1; XM_013224330.1.
DR   AlphaFoldDB; A0A2C9JRV7; -.
DR   STRING; 6526.A0A2C9JRV7; -.
DR   EnsemblMetazoa; BGLB007049-RB; BGLB007049-PB; BGLB007049.
DR   EnsemblMetazoa; BGLB007049-RC; BGLB007049-PC; BGLB007049.
DR   GeneID; 106065493; -.
DR   KEGG; bgt:106065493; -.
DR   VEuPathDB; VectorBase:BGLB007049; -.
DR   OrthoDB; 122704at2759; -.
DR   Proteomes; UP000076420; Unassembled WGS sequence.
DR   GO; GO:0102522; F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 3.30.300.110; Met-10+ protein-like domains; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR   InterPro; IPR030382; MeTrfase_TRM5/TYW2.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR23245; TRNA METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR23245:SF25; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 2 HOMOLOG; 1.
DR   Pfam; PF02475; Met_10; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51684; SAM_MT_TRM5_TYW2; 1.
PE   4: Predicted;
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          119..453
FT                   /note="SAM-dependent methyltransferase TRM5/TYW2-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51684"
SQ   SEQUENCE   462 AA;  51970 MW;  186F52786D395DE3 CRC64;
     MTDMALNEMG RSHVLIAESS NAQIIRRQLE ELHLWNGDFQ CRRISPNKVA LPVSLKGDNS
     QALKDKWPSL AVEEMDLTVS KKCQARKTAP SCRLREKLQL KLKEYSLSED LMRELPTKWE
     KHEDLILLPC SSFISPQWNI IADLWDVVAQ CLNCKRLARH STIAQNGFRS SQVKILLGKD
     GEVTHTDNGV KYSYDVTKCM FSAGNISEKL RLARLNCKGE IIVDLYAGIG YFTLPYLVHA
     KADHVHACEW NPDAVSALQN NLKLNGVSER CTVHYGDNRE LKLTSVADRV NLGLIPSSED
     GWPVAGQVLK DTGGVLHVHG NVTTNKQHNL QTDCSKIHHG TNNRERTTVV HSSDLTSNNE
     SLHHYQFGDT NCFDKLLETR ESNLDSSPVP SLDNTSTVSS ACSYWSSQVQ EKITILLETV
     KGGHWSVKEC HIELVKWYAP HIMHVVLDLE CRPRVAFLNN AN
//