ID A0A2C9M6T8_BIOGL Unreviewed; 768 AA.
AC A0A2C9M6T8;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=tRNA wybutosine-synthesizing protein 4 {ECO:0000256|ARBA:ARBA00018045};
DE EC=2.1.1.290 {ECO:0000256|ARBA:ARBA00012779};
DE EC=2.3.1.231 {ECO:0000256|ARBA:ARBA00012155};
DE AltName: Full=tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase {ECO:0000256|ARBA:ARBA00030847};
DE AltName: Full=tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase {ECO:0000256|ARBA:ARBA00029750};
GN Name=106078509 {ECO:0000313|EnsemblMetazoa:BGLB039233-PA};
OS Biomphalaria glabrata (Bloodfluke planorb) (Freshwater snail).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Heterobranchia; Euthyneura; Panpulmonata; Hygrophila; Lymnaeoidea;
OC Planorbidae; Biomphalaria.
OX NCBI_TaxID=6526 {ECO:0000313|EnsemblMetazoa:BGLB039233-PA, ECO:0000313|Proteomes:UP000076420};
RN [1] {ECO:0000313|EnsemblMetazoa:BGLB039233-PA}
RP IDENTIFICATION.
RC STRAIN=BB02 {ECO:0000313|EnsemblMetazoa:BGLB039233-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in
CC tRNA(Phe) + CO2 + S-adenosyl-L-methionine = 2 H(+) + S-adenosyl-L-
CC homocysteine + wybutosine(37) in tRNA(Phe); Xref=Rhea:RHEA:37119,
CC Rhea:RHEA-COMP:11844, Rhea:RHEA-COMP:11847, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73544, ChEBI:CHEBI:74275; EC=2.3.1.231;
CC Evidence={ECO:0000256|ARBA:ARBA00000401};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-
CC adenosyl-L-methionine = 7-[(3S)-(3-amino-3-
CC methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36903, Rhea:RHEA-COMP:10379, Rhea:RHEA-
CC COMP:11844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73543,
CC ChEBI:CHEBI:74275; EC=2.1.1.290;
CC Evidence={ECO:0000256|ARBA:ARBA00001806};
CC -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004797}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. LCMT family.
CC {ECO:0000256|ARBA:ARBA00010703}.
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DR RefSeq; XP_013094842.1; XM_013239388.1.
DR AlphaFoldDB; A0A2C9M6T8; -.
DR STRING; 6526.A0A2C9M6T8; -.
DR EnsemblMetazoa; BGLB039233-RA; BGLB039233-PA; BGLB039233.
DR KEGG; bgt:106078509; -.
DR VEuPathDB; VectorBase:BGLB039233; -.
DR OrthoDB; 5472060at2759; -.
DR UniPathway; UPA00375; -.
DR Proteomes; UP000076420; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 2.120.10.80; Kelch-type beta propeller; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR007213; Ppm1/Ppm2/Tcmp.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR46529; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4; 1.
DR PANTHER; PTHR46529:SF1; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4; 1.
DR Pfam; PF13415; Kelch_3; 1.
DR Pfam; PF04072; LCM; 1.
DR SUPFAM; SSF117281; Kelch motif; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
SQ SEQUENCE 768 AA; 86977 MW; 7FF69FE6C290A6C4 CRC64;
MHLPHDLAVQ GTNDSSIVSK CSTSTCGYFN DPYLHYFVQG TNDSSIVSKC STSTCGYFND
PYLHYFVSKQ SRRAPLIHRG YYIRAVAFDR ILKSFLELYK NSKKQVISLG CGFDSTYFRL
KSCNLLQNTV FCEIDFPEVV KKKRLLVENN EDLLKLVEKI KEQPLSPHIE LSSADYHLLG
VDLTQLNTLE AAIKMCGLEF DIPTLLLSEC VMTYMTRRCS TELVKWASET FEEAIFGMYE
QINPEDSFGL FMQNHFYSIG SPLKCINAFP SIESQRKRFL NTGWVCCEVY DMNTFYSLFV
DDKEKRRIES LETFDEYEEL NLKYSHYFIL TASTVELPRS LITYGHSEND KKIIPLEPAI
TGVLSFTYVP DGDQSLRRFG HASSQIYQHY TITTGGFGEI DGRHQRLAEI TVTDGRNFKS
CHVLCCSAHI QYSRMHHTSV PLSNGITYLF GGRQSPYFMC NQMLKLILTL DKHKQSCPGD
IGRARGTSSP IEIDHLVTGN GTFQGGDLEQ CNHSDDGQRN HSIDSIKHCD SNCDCCNSTF
SWDFELGTLQ VNVVAHNGKQ PRERWRHAAV AVTTDGKEKL FIHGGKTKTC DVLGDCHLFD
PETETWEEVL CSGDVPGPRH SHCVTFWNGN VLLTGGLDIH HEPVLDVFRL DIDSRVWYRM
NISGTFYPRY SHTAHLLGDF LILVGGVNTY HPPPGVAFVN LKTSQSMEFA LPSPDKTSLM
MLHRHTSILL DDQQLVVLGG GGNCFSFGTH LNRTPVLLNI SQCMKFFN
//
