ID A0A2C9VIJ0_MANES Unreviewed; 820 AA.
AC A0A2C9VIJ0;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=mitogen-activated protein kinase kinase kinase {ECO:0000256|ARBA:ARBA00012406};
DE EC=2.7.11.25 {ECO:0000256|ARBA:ARBA00012406};
GN ORFNames=MANES_07G044900 {ECO:0000313|EMBL:OAY45254.1};
OS Manihot esculenta (Cassava) (Jatropha manihot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae;
OC Manihot.
OX NCBI_TaxID=3983 {ECO:0000313|EMBL:OAY45254.1, ECO:0000313|Proteomes:UP000091857};
RN [1] {ECO:0000313|EMBL:OAY45254.1, ECO:0000313|Proteomes:UP000091857}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. AM560-2 {ECO:0000313|Proteomes:UP000091857};
RC TISSUE=Leaf {ECO:0000313|EMBL:OAY45254.1};
RA Bredeson J.V., Prochnik S.E., Lyons J.B., Schmutz J., Grimwood J.,
RA Vrebalov J., Bart R.S., Amuge T., Ferguson M.E., Green R., Putnam N.,
RA Stites J., Rounsley S., Rokhsar D.S.;
RT "WGS assembly of Manihot esculenta.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000478};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.25; Evidence={ECO:0000256|ARBA:ARBA00000106};
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr
CC protein kinase family. MAP kinase kinase kinase subfamily.
CC {ECO:0000256|ARBA:ARBA00006529}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00104}.
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DR EMBL; CM004393; OAY45254.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2C9VIJ0; -.
DR STRING; 3983.A0A2C9VIJ0; -.
DR EnsemblPlants; OAY45254; OAY45254; MANES_07G044900.
DR Gramene; OAY45254; OAY45254; MANES_07G044900.
DR Proteomes; UP000091857; Chromosome lg7.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004709; F:MAP kinase kinase kinase activity; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0000165; P:MAPK cascade; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd06606; STKc_MAPKKK; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR48016; MAP KINASE KINASE KINASE SSK2-RELATED-RELATED; 1.
DR PANTHER; PTHR48016:SF29; MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 1A; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR SMART; SM00220; S_TKc; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000091857};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 9..84
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000259|PROSITE:PS50053"
FT DOMAIN 388..480
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT DOMAIN 579..815
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT BINDING 608
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 820 AA; 95515 MW; AD18FA0111B06E9A CRC64;
MANSESNSFH LFVRMPKRKP IKILVKSNFE VQFIQQKILV ISKIPVTQQK LYYKGEKLEW
NKTLEDCSIQ NKAWLKLVVE LDGESELFQM IHQISSTICR MYREKSVSRI DYCPLAHKMF
ALLAPERNEQ SELLLIAYSV PATLVMFYRS RIKQQKAYAR ALILSSMHYL LNRPDDFLPE
CRLLALEFCK LLRHQVTNED PLYQSCRTSL KQLVERSRFK IKLFEEPETV LTICSFLYEM
TSTLCKGLVQ IRVSNPSSHI DSLKFLFGEF QAFSCVVRNA IGYIDGDDEE TLTCEREQFR
LLLKRQQSSL RLILETTTRE DDYHWLLEHN DVIDSESRMH LVTMMMIPEE KLLDAEFYKP
LIHWSRFLDE EMFEALKNKK LTSPKKLQDW LYKLCQVIFK PQNLLFLACP NDPTKFYPNP
ELKLEPLHFD CFEFSGKVIA LAVMHELQIG VAFHRMFLFQ LAEKDISIGD VKDAYPSFYN
KKAKECFLDD DQIRNDLVNS ISEQISFFRK GFDSVFGKSI VQLLSFKGIE LEDLNLVLKG
KLNLEFISGE ITHASDPLMS QFLKINRQGL NINKSEWRMD RKKTLGGGIS GNVYKGYADG
GFFFAVKKIR IKDKIKQEID RIQQEVNLLC QLRHPNIVKY YGSLRQVYKS FKLKDSQVSY
YTNQILEGLK YLHERKVVHR DIKCANILVD EKGCVKITDF GLAKVTELVP LLKSRHGTID
WIAPEVMKKD KEYGVEADIW SLGCTVLEML TGNYPYSHVN DWDANLELEV EKGTLRNHLP
NYSLSENARD FIMKCLQVDP KKRPTASQLL NHLFVKDSGC
//
