ID A0A2C9W3K1_MANES Unreviewed; 579 AA.
AC A0A2C9W3K1;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=cysteine--tRNA ligase {ECO:0000256|ARBA:ARBA00012832};
DE EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN ORFNames=MANES_03G012700 {ECO:0000313|EMBL:OAY53645.1};
OS Manihot esculenta (Cassava) (Jatropha manihot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae;
OC Manihot.
OX NCBI_TaxID=3983 {ECO:0000313|EMBL:OAY53645.1, ECO:0000313|Proteomes:UP000091857};
RN [1] {ECO:0000313|EMBL:OAY53645.1, ECO:0000313|Proteomes:UP000091857}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. AM560-2 {ECO:0000313|Proteomes:UP000091857};
RC TISSUE=Leaf {ECO:0000313|EMBL:OAY53645.1};
RA Bredeson J.V., Prochnik S.E., Lyons J.B., Schmutz J., Grimwood J.,
RA Vrebalov J., Bart R.S., Amuge T., Ferguson M.E., Green R., Putnam N.,
RA Stites J., Rounsley S., Rokhsar D.S.;
RT "WGS assembly of Manihot esculenta.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594}.
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DR EMBL; CM004389; OAY53645.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2C9W3K1; -.
DR STRING; 3983.A0A2C9W3K1; -.
DR EnsemblPlants; OAY53645; OAY53645; MANES_03G012700.
DR Gramene; OAY53645; OAY53645; MANES_03G012700.
DR Proteomes; UP000091857; Chromosome lg3.
DR GO; GO:0009507; C:chloroplast; IEA:EnsemblPlants.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042407; P:cristae formation; IEA:EnsemblPlants.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IBA:GO_Central.
DR GO; GO:0010197; P:polar nucleus fusion; IEA:EnsemblPlants.
DR GO; GO:0043067; P:regulation of programmed cell death; IEA:EnsemblPlants.
DR CDD; cd00672; CysRS_core; 1.
DR Gene3D; 1.20.120.1910; Cysteine-tRNA ligase, C-terminal anti-codon recognition domain; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00435; cysS; 1.
DR PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR PRINTS; PR00983; TRNASYNTHCYS.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000091857};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 94..391
FT /note="tRNA synthetases class I catalytic"
FT /evidence="ECO:0000259|Pfam:PF01406"
SQ SEQUENCE 579 AA; 65725 MW; 917839E2A3CFE39B CRC64;
MGSLIKCYKP LTSFRFSPPP HSLLRSIQFR THSSRTFSFS FSRKRTLCCL FSSLSSSWPL
INDKVNAGDG AITSSLSSAE LWLYNTMSRK KELFKPKVEG KVGMYVCGVT AYDLSHIGHA
RVYVTFDVLY RYLRHLGFEV CYVRNFTDVD DKIIARANEL GEDPISLSRR YCEEFHHDMV
YLHCLPPSVE PRVSDHMPQI IDMIKQILDS GCAYRMDGDV YFSVEKFPDY GQLSGRKLED
NRAGERVAVD ARKKNPADFA LWKSAKEGEP FWESPWGPGR PGWHIECSAM SAAYLGHSFD
IHGGGMDLVF PHHENEIAQS CAACRDSSIS YWVHNGFVTV DSEKMSKSLG NFFTIRQVID
LYHPLALRLF LLGTHYRSPI NYSDIQLESA SERIFYIYQT LHDCETVISQ HDMAFEKDSI
PPDILDGINR FHNVFVTSMS DDLHTPVLLA AFSDPLKIIN DMIHTRKGKK QAKRIESLVA
LEKIVKNALS VLGLMPTRYS EVLQQLREKA LKRAKLTEDQ VLQKIEGRAV ARKNRDYEKS
DAIRKDLATL GIALMDSPDG TSWRPAVPLA LQQQQVAAS
//
