ID A0A2D0Q8T1_ICTPU Unreviewed; 522 AA.
AC A0A2D0Q8T1;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=N-acetylgalactosamine-6-sulfatase {ECO:0000256|ARBA:ARBA00019527};
DE EC=3.1.6.4 {ECO:0000256|ARBA:ARBA00012117};
DE AltName: Full=Chondroitinsulfatase {ECO:0000256|ARBA:ARBA00033059};
DE AltName: Full=Galactose-6-sulfate sulfatase {ECO:0000256|ARBA:ARBA00030478};
DE AltName: Full=N-acetylgalactosamine-6-sulfate sulfatase {ECO:0000256|ARBA:ARBA00032952};
GN Name=galns {ECO:0000313|RefSeq:XP_017314699.1};
OS Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC Ictaluridae; Ictalurus.
OX NCBI_TaxID=7998 {ECO:0000313|RefSeq:XP_017314699.1};
RN [1] {ECO:0000313|RefSeq:XP_017314699.1}
RP IDENTIFICATION.
RC TISSUE=Blood {ECO:0000313|RefSeq:XP_017314699.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of the 6-sulfate groups of the N-acetyl-D-
CC galactosamine 6-sulfate units of chondroitin sulfate and of the D-
CC galactose 6-sulfate units of keratan sulfate.; EC=3.1.6.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000027};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR635626-2};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR635626-2};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000256|ARBA:ARBA00004371}.
CC -!- SIMILARITY: Belongs to the sulfatase family.
CC {ECO:0000256|ARBA:ARBA00008779}.
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DR RefSeq; XP_017314699.1; XM_017459210.3.
DR AlphaFoldDB; A0A2D0Q8T1; -.
DR STRING; 7998.ENSIPUP00000035146; -.
DR Ensembl; ENSIPUT00000036528; ENSIPUP00000035146; ENSIPUG00000023592.
DR GeneID; 108259597; -.
DR KEGG; ipu:108259597; -.
DR CTD; 2588; -.
DR OMA; YWEFHEG; -.
DR OrthoDB; 2913702at2759; -.
DR Proteomes; UP000221080; Chromosome 27.
DR GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043890; F:N-acetylgalactosamine-6-sulfatase activity; IEA:UniProtKB-EC.
DR CDD; cd16157; GALNS; 1.
DR Gene3D; 3.30.1120.10; -; 1.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 1.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR035626; GALNS.
DR InterPro; IPR024607; Sulfatase_CS.
DR InterPro; IPR000917; Sulfatase_N.
DR PANTHER; PTHR42693; ARYLSULFATASE FAMILY MEMBER; 1.
DR PANTHER; PTHR42693:SF50; N-ACETYLGALACTOSAMINE-6-SULFATASE; 1.
DR Pfam; PF00884; Sulfatase; 1.
DR Pfam; PF14707; Sulfatase_C; 1.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 1.
DR PROSITE; PS00523; SULFATASE_1; 1.
DR PROSITE; PS00149; SULFATASE_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR635626-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lysosome {ECO:0000256|ARBA:ARBA00023228};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR635626-2};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..522
FT /note="N-acetylgalactosamine-6-sulfatase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012519535"
FT DOMAIN 30..354
FT /note="Sulfatase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00884"
FT ACT_SITE 78
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR635626-1"
FT ACT_SITE 141
FT /evidence="ECO:0000256|PIRSR:PIRSR635626-1"
FT BINDING 38
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR635626-2"
FT BINDING 39
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR635626-2"
FT BINDING 78
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /note="via 3-oxoalanine"
FT /evidence="ECO:0000256|PIRSR:PIRSR635626-2"
FT BINDING 288
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR635626-2"
FT BINDING 289
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR635626-2"
FT MOD_RES 78
FT /note="3-oxoalanine (Cys)"
FT /evidence="ECO:0000256|PIRSR:PIRSR635626-4"
SQ SEQUENCE 522 AA; 58751 MW; 459174EF3674BD04 CRC64;
MEIRSVNYAF LLFWTISFIG TAETHNVSTP NIIIMLMDDM GWGDLGVFGE PSKETPHLDT
MASQGMLFPN FYSANPLCSP SRAALLTGRL PIRNGFYTTN AHARNAYTPQ EVVGGITKDE
ILLPQILKKQ GYVSKIVGKW HLGHREQHLP LEHGFDQFFG SPHCHFGPYK ESDRPNIPVY
NNSQMIGRYY EEFKINRKTG ESELTQYFLQ EALDFVSHQN TAHQPFFLYW APDATHDPVY
ASKQFLGQSQ RGLYGDAVME LDHSVGKILA HLVDLSIENN TFVFFTSDNG AAVMSGPGQS
GSNGPFLCGK ETTFEGGMRE PAIAWWPGRI PAGTVSYQLG TVMDLFTTSL ALAGLNAPED
RVIDGLDLSS TLFNKSLTER PVFYYRGNEM MAVRVGLYKA HYWTWTNSWE EFKQGVNFCP
GQEVLGVTNH TQQEHTMQPL IFHLGRDPGE KQPINVLSEE YLQVQRWINT VVEQHKVTLV
PGEPQLDMCD LAVMNWAPPG CEKLGKCLKP PPSHPQKCDW PH
//
