ID   A0A2D0QKR6_ICTPU        Unreviewed;       744 AA.
AC   A0A2D0QKR6;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   27-MAR-2024, entry version 26.
DE   RecName: Full=[heparan sulfate]-glucosamine N-sulfotransferase {ECO:0000256|ARBA:ARBA00012979};
DE            EC=2.8.2.8 {ECO:0000256|ARBA:ARBA00012979};
GN   Name=ndst2a {ECO:0000313|RefSeq:XP_017319063.1};
OS   Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC   Ictaluridae; Ictalurus.
OX   NCBI_TaxID=7998 {ECO:0000313|RefSeq:XP_017319063.1};
RN   [1] {ECO:0000313|RefSeq:XP_017319063.1}
RP   IDENTIFICATION.
RC   TISSUE=Blood {ECO:0000313|RefSeq:XP_017319063.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- PATHWAY: Glycan metabolism; heparan sulfate biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00005093}.
CC   -!- PATHWAY: Glycan metabolism; heparin biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00004841}.
CC   -!- SIMILARITY: Belongs to the sulfotransferase 1 family. NDST subfamily.
CC       {ECO:0000256|ARBA:ARBA00010420}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   RefSeq; XP_017319063.1; XM_017463574.3.
DR   AlphaFoldDB; A0A2D0QKR6; -.
DR   GeneID; 108263104; -.
DR   CTD; 793524; -.
DR   OrthoDB; 2913264at2759; -.
DR   UniPathway; UPA00756; -.
DR   UniPathway; UPA00862; -.
DR   Proteomes; UP000221080; Chromosome 3.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015016; F:[heparan sulfate]-glucosamine N-sulfotransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019213; F:deacetylase activity; IEA:UniProt.
DR   GO; GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0030210; P:heparin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR021930; Heparan_SO4_deacetylase.
DR   InterPro; IPR037359; NST/OST.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000863; Sulfotransferase_dom.
DR   PANTHER; PTHR10605:SF53; BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE 2; 1.
DR   PANTHER; PTHR10605; HEPARAN SULFATE SULFOTRANSFERASE; 1.
DR   Pfam; PF12062; HSNSD; 1.
DR   Pfam; PF00685; Sulfotransfer_1; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        18..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          89..519
FT                   /note="Heparan sulphate-N-deacetylase"
FT                   /evidence="ECO:0000259|Pfam:PF12062"
FT   DOMAIN          609..685
FT                   /note="Sulfotransferase"
FT                   /evidence="ECO:0000259|Pfam:PF00685"
FT   REGION          708..744
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        716..744
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        618
FT                   /note="For sulfotransferase activity"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR637359-1"
SQ   SEQUENCE   744 AA;  84740 MW;  A5CFEB293B8084D0 CRC64;
     MGLWKLLRGI RQLELHRLIL VLIIFCLLSM ALLAYYVSNS PKIKEAPPLP FSDCGEVVAA
     GDGLQRVPLF LPPFVGRQRT VKTVDNSRTE PVVLVFIESI YSQLGQEIVA ILESSRFRYH
     TEIAPSKGDM PTLMERDRGR YALVIYENVL KYVNLDAWNR DLLDKYCSEF GVGIIGFFKA
     NENSLLSAQL KGFPLFLHSN LGLRDYRINP VAPLLYVTKP NEVEQGPLPG DDWTVFQSNH
     STYEPVLLAS TKSSEALAHL GPHRVLHATV VQDLGLHDGI QRVLFGNNLS FWLHKLIFVD
     SIAYLTGKRL CLSLERHILV DVDDIFVGKE GTRMKVSDVK ALLNTQTKLR TLVPNFTFNL
     GFSGKFYHAG TEEEDEGDDM LMQHKEEFWW FPHMWSHMQP HLFHNVSVLS EQMRLNKLFA
     QEHGIPTDMG YAVSPHHSGV YPVHSQLYEA WKNIWGIQVT STEEYPHLRP ARHRRGFIHN
     DIQVLPRQTC GLFTHTIFYN EYPGGSRELD KSIRGGELFL TVLLNPISIF MTHLSNYGND
     RLGLYTFESL VKFVQCWTNL RLQTLPPLEL AEKYFQIFPE ERDPLWQNPC HDKRHKDIWS
     KEKTCDRLPK FLVIGPQKTG TTALHSFLTL HPAITSSFPS PVTFEEIQFF NGPSYHNGID
     WYDVSVVFDC PSSHAGTWTS SPSPRTPVQT SCLRKVLTTL TQRWSPRGLQ PYCPGPRSSL
     CSSTQPTEPT PGTSTSGLIK THLL
//