ID A0A2D0RHS5_ICTPU Unreviewed; 719 AA.
AC A0A2D0RHS5;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN Name=mid2 {ECO:0000313|RefSeq:XP_017330084.1,
GN ECO:0000313|RefSeq:XP_017330085.1, ECO:0000313|RefSeq:XP_017330087.1,
GN ECO:0000313|RefSeq:XP_017330088.1, ECO:0000313|RefSeq:XP_053538000.1};
OS Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC Ictaluridae; Ictalurus.
OX NCBI_TaxID=7998 {ECO:0000313|RefSeq:XP_017330087.1};
RN [1] {ECO:0000313|RefSeq:XP_017330084.1, ECO:0000313|RefSeq:XP_017330085.1}
RP IDENTIFICATION.
RC TISSUE=Blood {ECO:0000313|RefSeq:XP_017330084.1,
RC ECO:0000313|RefSeq:XP_017330085.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000256|ARBA:ARBA00004245}.
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DR RefSeq; XP_017330084.1; XM_017474595.3.
DR RefSeq; XP_017330085.1; XM_017474596.3.
DR RefSeq; XP_017330086.1; XM_017474597.1.
DR RefSeq; XP_017330087.1; XM_017474598.3.
DR RefSeq; XP_017330088.1; XM_017474599.3.
DR RefSeq; XP_053538000.1; XM_053682025.1.
DR Ensembl; ENSIPUT00000000824; ENSIPUP00000000769; ENSIPUG00000000550.
DR GeneID; 108269068; -.
DR KEGG; ipu:108269068; -.
DR CTD; 11043; -.
DR OrthoDB; 5383069at2759; -.
DR Proteomes; UP000221080; Chromosome 8.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR CDD; cd00063; FN3; 1.
DR CDD; cd13739; SPRY_PRY_TRIM1; 1.
DR Gene3D; 1.20.5.170; -; 1.
DR Gene3D; 2.60.120.920; -; 1.
DR Gene3D; 4.10.830.40; -; 1.
DR Gene3D; 3.30.160.60; Classic Zinc Finger; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR003649; Bbox_C.
DR InterPro; IPR003879; Butyrophylin_SPRY.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR017903; COS_domain.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR040859; Midline-1_COS.
DR InterPro; IPR035752; SPRY/PRY_TRIM1.
DR InterPro; IPR003877; SPRY_dom.
DR InterPro; IPR027370; Znf-RING_euk.
DR InterPro; IPR000315; Znf_B-box.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR24099:SF12; E3 UBIQUITIN-PROTEIN LIGASE MID2-RELATED; 1.
DR PANTHER; PTHR24099; E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED; 1.
DR Pfam; PF18568; COS; 1.
DR Pfam; PF00041; fn3; 1.
DR Pfam; PF00622; SPRY; 1.
DR Pfam; PF00643; zf-B_box; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR PRINTS; PR01407; BUTYPHLNCDUF.
DR SMART; SM00502; BBC; 1.
DR SMART; SM00336; BBOX; 2.
DR SMART; SM00060; FN3; 1.
DR SMART; SM00184; RING; 1.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF57845; B-box zinc-binding domain; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR SUPFAM; SSF49265; Fibronectin type III; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS50188; B302_SPRY; 1.
DR PROSITE; PS51262; COS; 1.
DR PROSITE; PS50853; FN3; 1.
DR PROSITE; PS50119; ZF_BBOX; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Cytoskeleton {ECO:0000256|ARBA:ARBA00023212};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Microtubule {ECO:0000256|ARBA:ARBA00022701};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00024}.
FT DOMAIN 10..60
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT DOMAIN 182..224
FT /note="B box-type"
FT /evidence="ECO:0000259|PROSITE:PS50119"
FT DOMAIN 354..413
FT /note="COS"
FT /evidence="ECO:0000259|PROSITE:PS51262"
FT DOMAIN 415..518
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000259|PROSITE:PS50853"
FT DOMAIN 500..693
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000259|PROSITE:PS50188"
FT REGION 88..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 698..719
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 254..288
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 699..719
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 719 AA; 80387 MW; 10C2976BA238F9E5 CRC64;
METLESELTC PICLELFEDP LLLPCAHSLC FSCAHRILVS HCSGAKPLES VSAFQCPTCR
HVITLSPRGL DGLRRNVTLQ NIIDRFQKAS FSGPGSPNES RLRPPPRPRS ATPTGGSANG
SAMSAELRVP CQFCEQEPPR DAVKTCATCQ ASYCERCLRA THPNKKPFTS HRLLEPAGDG
HLRGLACLEH DSETVSLYCA TDEQLICTLC KLVGRHREHH VASLSERFDK LKGFHPSPGR
VSEAVMEASK TSAAQTLENN LTNLVKRNSE LENQMAKLIQ ICQQVEVNTA MHEAKLVEEC
DELVEIIRQR KQVIAVKIKE SKVMKLRKLA QQIANCRQCL ERSSALITQA EQSLKENDHA
RFLQTARNVA ERVAMATASS QVLIPDVNLN EAFDNFALDF SREKKILEGL DYLTAPNPPS
IRDELCTASH DTITVHWTSE DEFSVTSYEL QYTIYTGQTN FISLYNSVDS WMIVPNIKQN
HYTVHGLQSG TRYIFFVKAI NQAGSRNSEP ARLKTNSQPF KLDPKTAHKK LRLSNDCLTM
EKDESSLKKS HTPERFSGTG SYGAAGNVFI DSGCHYWEVL LGASTWYTVG VAYKSAPKNE
WNGKNSSSWV FSRCNNNFLV RHNSKEMLVE ASLQLRRLGV LLDYDNNTLS FYDAMNSQHL
HTFDISFLLP VAPTFMISNK SLMILSGLPV PDFVDCPEQQ DGSSRQHESP YGLTMKSCN
//
