ID A0A2D0S920_ICTPU Unreviewed; 2222 AA.
AC A0A2D0S920;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Phosphoinositide phospholipase C {ECO:0000256|RuleBase:RU361133};
DE EC=3.1.4.11 {ECO:0000256|RuleBase:RU361133};
GN Name=plce1 {ECO:0000313|RefSeq:XP_047015538.1,
GN ECO:0000313|RefSeq:XP_047015539.1, ECO:0000313|RefSeq:XP_047015540.1};
OS Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC Ictaluridae; Ictalurus.
OX NCBI_TaxID=7998 {ECO:0000313|RefSeq:XP_047015539.1};
RN [1] {ECO:0000313|RefSeq:XP_047015538.1, ECO:0000313|RefSeq:XP_047015539.1}
RP IDENTIFICATION.
RC TISSUE=Blood {ECO:0000313|RefSeq:XP_047015538.1,
RC ECO:0000313|RefSeq:XP_047015539.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-
CC bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-
CC diacyl-sn-glycerol + H(+); Xref=Rhea:RHEA:33179, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:58456,
CC ChEBI:CHEBI:203600; EC=3.1.4.11;
CC Evidence={ECO:0000256|RuleBase:RU361133};
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DR RefSeq; XP_017338936.1; XM_017483447.1.
DR RefSeq; XP_047015538.1; XM_047159582.2.
DR RefSeq; XP_047015539.1; XM_047159583.2.
DR RefSeq; XP_047015540.1; XM_047159584.2.
DR STRING; 7998.ENSIPUP00000016635; -.
DR KEGG; ipu:108273837; -.
DR Proteomes; UP000221080; Chromosome 13.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0004435; F:phosphatidylinositol phospholipase C activity; IEA:UniProtKB-EC.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00275; C2_PLC_like; 1.
DR CDD; cd16203; EFh_PI-PLCepsilon; 1.
DR CDD; cd08596; PI-PLCc_epsilon; 1.
DR CDD; cd01780; RA2_PLC-epsilon; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 3.20.20.190; Phosphatidylinositol (PI) phosphodiesterase; 1.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR001192; PI-PLC_fam.
DR InterPro; IPR046973; PLC-epsilon1_cat.
DR InterPro; IPR028398; PLC-epsilon1_RA2.
DR InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR InterPro; IPR015359; PLC_EF-hand-like.
DR InterPro; IPR046974; PLC_epsilon1_EF.
DR InterPro; IPR000909; PLipase_C_PInositol-sp_X_dom.
DR InterPro; IPR001711; PLipase_C_Pinositol-sp_Y.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10336:SF6; 1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE EPSILON-1; 1.
DR PANTHER; PTHR10336; PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF09279; EF-hand_like; 1.
DR Pfam; PF00388; PI-PLC-X; 1.
DR Pfam; PF00387; PI-PLC-Y; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR PRINTS; PR00390; PHPHLIPASEC.
DR SMART; SM00239; C2; 1.
DR SMART; SM00148; PLCXc; 1.
DR SMART; SM00149; PLCYc; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF51695; PLC-like phosphodiesterases; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 2.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50007; PIPLC_X_DOMAIN; 1.
DR PROSITE; PS50008; PIPLC_Y_DOMAIN; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
PE 4: Predicted;
KW Guanine-nucleotide releasing factor {ECO:0000256|PROSITE-ProRule:PRU00168};
KW Hydrolase {ECO:0000256|RuleBase:RU361133};
KW Lipid degradation {ECO:0000256|RuleBase:RU361133};
KW Lipid metabolism {ECO:0000256|RuleBase:RU361133};
KW Transducer {ECO:0000256|ARBA:ARBA00023224}.
FT DOMAIN 467..720
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT DOMAIN 1691..1781
FT /note="PI-PLC Y-box"
FT /evidence="ECO:0000259|PROSITE:PS50008"
FT DOMAIN 1787..1912
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 2064..2169
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT REGION 248..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 750..775
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 979..1077
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1089..1117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1533..1563
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1618..1679
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2192..2222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 248..265
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 753..767
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 979..1001
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1007..1045
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1052..1077
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1089..1112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1539..1563
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1664..1679
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2222 AA; 247205 MW; 9F31E665CFAF61D7 CRC64;
MRVCVEEQVG IGLPSISVSS EKAAVILGVG MEARAASSLT KFRNQMLSFG YEAHERRNSD
PLMFCSCSHS LHPAEDCCDS KSSEQIRMDC GTRSPLDSRS KSLDSLDYLH KDCCCSTVSC
RRVEGLCCSQ PCSTCGPVET DGCCQEAAVH SGGWESGFPR RTEGFSSAAT RLAGESPSLD
EERLGTRRIS LATLFLSRFH CTCAYGARLE AGTQGQVNFN SAHCHNGVFS SLERLNKRSK
VRCSRMPCQA STTSKPSLAH QHSDGEATAS DSEFTHKKER STVLVRRYLK NNQKVLKKVC
TGTRAIVRTG HISDRAWQAA YGRRCRLSKA YTFVDTEYSR VLHAIQLQVS RALLSYSGVR
LEQLQYAFPA LNAEVHLLHP SRKLLARAVA WLPTCVQHTE HRVCSCRTRL AAHLSGALME
ATAAITTSTP MFKERQLCGS TASSGSLSSG VMGISKELAD LRQLIQFPEE IASILTEQEQ
QLYRRVLPLD YLCFLTRDLA SPDCHKSHPH LKASLSAPVM PTQSSQHNVV EDLVARFNEV
SSWVTWLILT AGSMEEKREV FSYLVHIAKC CWNMGNYNAV MEFLAGLRSR KVLKMWQFMD
QADIETMRGL KDAMAQHESS SEYKKVVNRA LNIPGCKVVP FCGVFLKELS EALDGAASLI
GLRPAFDSQE DSIEFVSDYN GQQHFLQRAG ADGLHNSEKE ATVSNILQTI RSCNRSLEAD
EPEDAFRDRS KNQRASDSGR VLFLVGDLSD SEGDLTEQPR ESDLQSPEEP EGAFSHGTEV
VPWYILSLSA DMHRFLLQGA TVIHYEQESH LTARCLLRLQ PDNCTLTWIK PPSSRSRGVV
PDSLLQGQVA VCGLAEGILD LSAVKAVFMG HPGVDINVVR LQHKLCGLSA EENGVTLLYG
LHTTDNRLLH FVAPWHTASV LYEGLRELIS GIRKMKRFPD QRLQWLRRQY VSLYQEDGRF
EGPTLAQAIE LFGGRRWNMG SGSRSTEKTT TQKNSPLGIS SNVKKKKKAL VRGDSGDGTD
DEMVSRKTKS CKEGLSRRSS DPVDSVEQEE NDDSSSFGTP GSSFSSSTKT QSPGSSSFSS
PFCSSSINSS TPIRPHSSPV LPCTSKGQPG AWSSRSWHGR GKGCFRGFQN LMISDSIMSF
VEFVELFKSF SIRSRKDIKE LFDTYAVPCT RSGPDSVPLY TTLRIDDKIT GLQPDLDLLT
RNGSDLGLFI RTRQQMSENQ KQISDAIAAA SIVSNGTGVE HSSLGVLGLA IPQLNDFLVN
CQREHLTYDE ILTIIQKFEP SSSMRQMGWM SFEGFARFLM DKDNFASKNE ESQVNSDELQ
YPLSYYYIES SHNTYLTGHQ LKGESSVELY SQVLLQGCRS VELDCWDGDD GMPVIYHGHT
LTTKIPFKDV VEAINRSAFV NSEMPVILSI ENHCSLPQQR KMADIFKVVF GDKLVTKFLF
ESDFSDDPLL PSPWQLRGKI LLKNKKLKAH QAPVDILKQK ALQLAHMQAQ ANNCTVAAGS
LGNNEEEEEE EDEYDYDYES LSDADVLNAS TASYSQEDNI LDDKPEGKSS TEKLQYESND
EMPKRIKKAG SKGKMFDMEL GEEFYLPQNE KESRQIAQEL SDLVIYCQAI KFPGLSTHSP
SCSNRGKERK SRKSIFGNAP VRGSPGEPVT LVRGSGKASM DGARSSWEEQ TMPTTGTTPS
LSAIIRTPKC YHISSVNENA AKRLCRRYSA KLIGHTERQL LRTYPAATRI DSANPNPLCF
WLHGIQLVAL NYQTDDLPMQ LNTALFEVNG HCGFVLKPPV LWDRTCYLYH QFSPLERDLE
HMSPTVYSLT VVSGQNVCPG NSNGSPCVEV EVLSLPADAC HFRTKPIHRN TLNPMWSESF
TFQVHFEDLT FLRLAVVENN SSQVTAQRIL PLKALKPGYR HLQLRNQHNE ALEVSTLFLF
SRKTEESPDG GARPAVVLFS SEERRVAQQH KVTVHGAPGP EPFTVLSMKE HTTAKQLLNV
LVPDASADYF LMEEKIPLSK EKCDAKKVPQ QRPLASDERV LKVLHSWQPE EGYVGRLCLK
AKEENLNEKN DKMERAEEAS SGGDEDVFFV QVHDVSPEQP RTVIKAPRYS TAQDIIQQAL
SKAKYSVSIL SNPNPSDYVL MEEVGREAPG KKVSSAKPAQ RVLPDHECVF QAQSKWKGPG
KFILKLKEQI GREDKRKCVS FASELWKLTG RSRSVTMNGP GSSEAAHSKE ERGAPPPPDT
LD
//
