ID   A0A2D5Z6V4_9BACT        Unreviewed;       527 AA.
AC   A0A2D5Z6V4;
DT   31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT   31-JAN-2018, sequence version 1.
DT   24-JAN-2024, entry version 20.
DE   RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
GN   ORFNames=CMJ18_11315 {ECO:0000313|EMBL:MAE64847.1};
OS   Phycisphaeraceae bacterium.
OC   Bacteria; Planctomycetota; Phycisphaerae; Phycisphaerales;
OC   Phycisphaeraceae.
OX   NCBI_TaxID=2026777 {ECO:0000313|EMBL:MAE64847.1, ECO:0000313|Proteomes:UP000222953};
RN   [1] {ECO:0000313|Proteomes:UP000222953}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Tully B.J., Graham E.D., Heidelberg J.F.;
RT   "The Reconstruction of 2,631 Draft Metagenome-Assembled Genomes from the
RT   Global Oceans.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC         methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC         Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC         ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC         Evidence={ECO:0000256|ARBA:ARBA00034420};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC         Evidence={ECO:0000256|ARBA:ARBA00034420};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974,
CC         ECO:0000256|RuleBase:RU003862};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC       {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:MAE64847.1}.
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DR   EMBL; NYZC01000178; MAE64847.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2D5Z6V4; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000222953; Unassembled WGS sequence.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.20.220; -; 1.
DR   InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR   InterPro; IPR003171; Mehydrof_redctse-like.
DR   InterPro; IPR022026; MTHFR_C.
DR   PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR   PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR   Pfam; PF12225; DUF5981; 1.
DR   Pfam; PF02219; MTHFR; 1.
DR   SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW   ECO:0000256|RuleBase:RU003862};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000256|RuleBase:RU003862}.
FT   DOMAIN          410..487
FT                   /note="Methylene-tetrahydrofolate reductase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12225"
FT   REGION          506..527
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        507..527
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   527 AA;  58519 MW;  DB28BB2A24926D01 CRC64;
     MLDASGFLIG VELVTTRGTM GQVTATRTRR LASDLVGCDR VDWVSITDNA GGNPMLSPEA
     LGRPVLYGGK EVMIHLSCKD MNRHGLESQA WHLASEGFSN ILALSGDYPA EGYHGRAKPV
     FDIDSVGLLS LLDEMNRGLG KRPAVDANGE QIKGHRGGTD FFCGAVTTNF KLRENEVVPQ
     LLKLEKKIAC GARFVIAQIG YDSRKSHELL CYMQRRGHGD VPIFGNVFVL SRPAVRFFRR
     GGIPGVVISD ELHTECAAQG AAPDRGRRFF LELAARQIAI FRGLGYRGAY IGGVHDLGAI
     EEILDIEASY SADDWKQFAR AVRYPRPNEF FLYREKPETG LADPDTRHPA YQDALDNAGA
     NHNVTLAYRL SRAMHSLVFT PGKPLYRLAR RIYERSSNER RGPVALRVLE HAGKSVMFGC
     KDCGDCSLPD IAYLCPESQC AKNQRNGPCG GTRDGRCEVH DYECIWARAY DRLKWEQRQD
     HLLDHAPVIQ DEALRGSSSW ASLYLGRDHH GRNRPSPDDE ATREDSS
//