ID A0A2G1X1V4_9EURY Unreviewed; 227 AA.
AC A0A2G1X1V4;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Adenine DNA glycosylase {ECO:0000256|ARBA:ARBA00022023};
DE EC=3.2.2.31 {ECO:0000256|ARBA:ARBA00012045};
DE Flags: Fragment;
GN ORFNames=DJ68_14195 {ECO:0000313|EMBL:PHQ45206.1};
OS Halorubrum sp. C3.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Halorubraceae; Halorubrum.
OX NCBI_TaxID=1483399 {ECO:0000313|EMBL:PHQ45206.1, ECO:0000313|Proteomes:UP000225238};
RN [1] {ECO:0000313|EMBL:PHQ45206.1, ECO:0000313|Proteomes:UP000225238}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C3 {ECO:0000313|EMBL:PHQ45206.1,
RC ECO:0000313|Proteomes:UP000225238};
RX PubMed=24782836; DOI=10.3389/fmicb.2014.00140;
RA Fullmer M.S., Soucy S.M., Swithers K.S., Makkay A.M., Wheeler R.,
RA Ventosa A., Gogarten J.P., Papke R.T.;
RT "Population and genomic analysis of the genus Halorubrum.";
RL Front. Microbiol. 5:140-140(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolyzes free adenine bases from 7,8-dihydro-8-
CC oxoguanine:adenine mismatched double-stranded DNA, leaving an
CC apurinic site.; EC=3.2.2.31;
CC Evidence={ECO:0000256|ARBA:ARBA00000843};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the Nth/MutY family.
CC {ECO:0000256|ARBA:ARBA00008343}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PHQ45206.1}.
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DR EMBL; NHOB01000862; PHQ45206.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G1X1V4; -.
DR Proteomes; UP000225238; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0000701; F:purine-specific mismatch base pair DNA N-glycosylase activity; IEA:UniProtKB-EC.
DR GO; GO:0006284; P:base-excision repair; IEA:InterPro.
DR CDD; cd00056; ENDO3c; 1.
DR Gene3D; 1.10.1670.10; Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal); 1.
DR InterPro; IPR011257; DNA_glycosylase.
DR InterPro; IPR003265; HhH-GPD_domain.
DR InterPro; IPR023170; HhH_base_excis_C.
DR InterPro; IPR000445; HhH_motif.
DR InterPro; IPR044298; MIG/MutY.
DR PANTHER; PTHR42944; ADENINE DNA GLYCOSYLASE; 1.
DR PANTHER; PTHR42944:SF1; ADENINE DNA GLYCOSYLASE; 1.
DR Pfam; PF00633; HHH; 1.
DR Pfam; PF00730; HhH-GPD; 1.
DR SMART; SM00478; ENDO3c; 1.
DR SUPFAM; SSF48150; DNA-glycosylase; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 33..180
FT /note="HhH-GPD"
FT /evidence="ECO:0000259|SMART:SM00478"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:PHQ45206.1"
FT NON_TER 227
FT /evidence="ECO:0000313|EMBL:PHQ45206.1"
SQ SEQUENCE 227 AA; 25510 MW; 6C19584BEB1BCB55 CRC64;
VRDALVDWYE ADHRDFPWRR TEDPYEILVS EVMSQQTQLD RVVPAWEDFL DEWPTTAALA
AADRADVVAF WSDHSLGYNN RAKYLHEAAG QVEDDHDGAF PEDPDGLSEL MGVGPYTANA
VASFAFDNGD AVVDTNVKRV LYRAFDVPDD DDAFERVASQ LMPAGESRVW NNVIMELGGV
ACGKKPRCDE AGCPWREWCH AYQTGDFTAP DVPEQPSFEG SRRQFRG
//
