ID A0A2G1XB21_STRCJ Unreviewed; 1159 AA.
AC A0A2G1XB21;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
DE AltName: Full=Error-prone DNA polymerase {ECO:0000256|ARBA:ARBA00017273};
GN ORFNames=BLA24_29170 {ECO:0000313|EMBL:PHQ48447.1}, CYQ11_06305
GN {ECO:0000313|EMBL:PPT12557.1};
OS Streptomyces cinnamoneus (Streptoverticillium cinnamoneum).
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces; Streptomyces cinnamoneus group.
OX NCBI_TaxID=53446 {ECO:0000313|EMBL:PHQ48447.1, ECO:0000313|Proteomes:UP000222531};
RN [1] {ECO:0000313|EMBL:PHQ48447.1, ECO:0000313|Proteomes:UP000222531}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 21532 {ECO:0000313|EMBL:PHQ48447.1,
RC ECO:0000313|Proteomes:UP000222531};
RX PubMed=29053243;
RA Patteson J., Cai W., Johnson R.A., Santa Maria K., Li B.;
RT "Identification of the Biosynthetic Pathway for the Antibiotic
RT Bicyclomycin.";
RL Biochemistry 0:0-0(2017).
RN [2] {ECO:0000313|EMBL:PPT12557.1, ECO:0000313|Proteomes:UP000239771}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 41675 {ECO:0000313|EMBL:PPT12557.1,
RC ECO:0000313|Proteomes:UP000239771};
RA Vior N.M., Lacret R., Chandra G., Dorai-Raj S., Trick M., Truman A.W.;
RT "Discovery and biosynthesis of the antibiotic bicyclomycin in distant
RT bacterial classes.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|ARBA:ARBA00007391}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PHQ48447.1}.
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DR EMBL; NHZO01000162; PHQ48447.1; -; Genomic_DNA.
DR EMBL; PKFQ01000001; PPT12557.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G1XB21; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000222531; Unassembled WGS sequence.
DR Proteomes; UP000239771; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000222531};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 5..72
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1159 AA; 124620 MW; C5009BF3D35964A2 CRC64;
MAGFTHLHTA SGFSMRYGAA HPELLAERAA ERGMDAVALT DRDSLAGAVR FARASARAGV
RPLFGVDLAV REPLPAPGPT ARHRAPVRGG AFVDESAPRA VFLARDGAAG WAALCGLVSA
AHAGDASRAG GSGAGAARPE LDRTDLAEAA AGLRAPLTVL LGPDSEVGRA LAAGRPDRAA
RLLAPWREMF GDGLRLEVVW HGRTGPGTGP GSLRLAARTA GFAADQGVRA VLTNATRYAD
PGQGPVADVL DSARRLVPID TRRPEALDGG ERWLKDPAAM AAVADRVVEA AGFRRAAAHR
LLAMTQEAAA ECLVDPEDDL GLGSVHFPEP HLVGAGRRSA ARVLRSRCAG AMVLRGHDRD
RRYWDRLEEE LRVIEARGFP SYFLTVAQVV DDTRALGIRV TARGSGAGSL VNHLLGIAHA
DPVAHGLLME RFLSVRRAAL PDIDIDVESA RRLDVYRAVF DRFGAERVAT VSMPETYRVR
HAVRDVGAAL GMDPAEVDRL AKAFPHIRAR DARAALAELP ELRGVAAEEH GRLWELVEAL
DALPRGIAMH PCGVLLSDAS LRARTPVVPT SGEGFAMSQF DKDDVEELGL LKLDVLGVRM
QSAMAHAVAE IERATGRRID LDDPAQVPPG DRRTYELIRS TETLGCFQIE SPGQRDLVGR
LQPADFHDLV VDISLFRPGP VAADMVRPFV EARHGRAPVR LPHPDLAETL GETYGVVVFH
EQIIEILRIM TGCDRGFADE TRRALSDPEL LGRVRTWFAG RARERGYAPD VVRRTWEIVE
AFGSYGFCKA HAVAFAVPTY QSAWLKAHHP AAFYAGLLTH DPGMYPKRLL LADARRRGVP
ILPLDVNRSG ATHTIELVSE KEGFGIRLAL SEVQGISEAE TRRIVAGRPY TSLQDLWRRA
RPARPVAERL ARVGALDAFG TNRRDLLLHI AELHRHGRAG SADQPTLIDS ARAEPVGLPD
LDAAERLGAE LNVLGMDTSR HLMSDHRAFL AELGAIPARR LRDARHGETV LVAGAKAATQ
TPPVRSGRRV IFTTLDDGTG LVDCAFFDDS HEACAHTVFH SWLLLVRGVV QRRGPRSLSV
VGAAAWNLAE LTALRAEGGL GAVAAVLADG TAPAAPAPGR RIRMSTGYTM HPWADLRPAG
EGAATGRKLW HASQGSAGR
//
