ID A0A2G2WCN0_CAPBA Unreviewed; 766 AA.
AC A0A2G2WCN0;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Isoamylase 3, chloroplastic {ECO:0000313|EMBL:PHT42962.1};
GN ORFNames=CQW23_16987 {ECO:0000313|EMBL:PHT42962.1};
OS Capsicum baccatum (Peruvian pepper).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum.
OX NCBI_TaxID=33114 {ECO:0000313|EMBL:PHT42962.1, ECO:0000313|Proteomes:UP000224567};
RN [1] {ECO:0000313|EMBL:PHT42962.1, ECO:0000313|Proteomes:UP000224567}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. PBC81 {ECO:0000313|Proteomes:UP000224567};
RC TISSUE=Leaf {ECO:0000313|EMBL:PHT42962.1};
RX PubMed=29089032; DOI=10.1186/s13059-017-1341-9;
RA Kim S., Park J., Yeom S.I., Kim Y.M., Seo E., Kim K.T., Kim M.S., Lee J.M.,
RA Cheong K., Shin H.S., Kim S.B., Han K., Lee J., Park M., Lee H.A.,
RA Lee H.Y., Lee Y., Oh S., Lee J.H., Choi E., Choi E., Lee S.E., Jeon J.,
RA Kim H., Choi G., Song H., Lee J., Lee S.C., Kwon J.K., Lee H.Y., Koo N.,
RA Hong Y., Kim R.W., Kang W.H., Huh J.H., Kang B.C., Yang T.J., Lee Y.H.,
RA Bennetzen J.L., Choi D.;
RT "New reference genome sequences of hot pepper reveal the massive evolution
RT of plant disease-resistance genes by retroduplication.";
RL Genome Biol. 18:R210.1-R210.11(2017).
RN [2] {ECO:0000313|Proteomes:UP000224567}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. PBC81 {ECO:0000313|Proteomes:UP000224567};
RA Kim S., Park J., Yeom S.-I., Kim Y.-M., Seo E., Kim K.-T., Kim M.-S.,
RA Lee J.M., Cheong K., Shin H.-S., Kim S.-B., Han K., Lee J., Park M.,
RA Lee H.-A., Lee H.-Y., Lee Y., Oh S., Lee J.H., Choi E., Choi E., Lee S.E.,
RA Jeon J., Kim H., Choi G., Song H., Lee J., Lee S.-C., Kwon J.-K.,
RA Lee H.-Y., Koo N., Hong Y., Kim R.W., Kang W.-H., Huh J.H., Kang B.-C.,
RA Yang T.-J., Lee Y.-H., Bennetzen J.L., Choi D.;
RT "Multiple reference genome sequences of hot pepper reveal the massive
RT evolution of plant disease resistance genes by retroduplication.";
RL J. Anim. Genet. bioRxivorg:115410-115410(2017).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PHT42962.1}.
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DR EMBL; MLFT02000007; PHT42962.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G2WCN0; -.
DR STRING; 33114.A0A2G2WCN0; -.
DR Proteomes; UP000224567; Chromosome 7.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0043170; P:macromolecule metabolic process; IEA:UniProt.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR048650; ISOA1-3-like_C.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF3; ISOAMYLASE 3, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21156; ISOA1-3_C; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR PROSITE; PS50890; PUA; 1.
PE 4: Predicted;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000224567};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 240..658
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 766 AA; 86667 MW; C7CC1028B7ECEC0D CRC64;
MTASPAVAIL QSSSTNFNSS FYTSLYSSSL LRLNSSRRPL QEVFGQKSIS KAGERYNIRA
AKVYSLRAEE QRVIQEETPQ MLDTFPSFEV SPGLAHPLGV SEVESGINFA IFSQHASAVT
LCIILPKSIH DGVIELALDP QKNRTGNIWH ICIKELPQGD VLYGYRIDGP RNWHEGHRFD
NSIILIDPYA KLIEGRRAFG DNSSKMCRFL GTYDFNSVPF DWGENYKLPN ILENDLVIYE
INVRAFTADE TSGLDLNQRG SYLGLIEKIP HLLELGVNAV ELLPVFEFDE LEFQRRPNPR
DHMINTWGYS TMNFFAPMSR YASCGGGPVG ASREFKEMVK ALHGAGIEVI LDVVYNHTNE
ADDENPYTTS FRGIDNKVYY MMDLNNNAQL VNFSGCGNTF NCNHPVVMEL ILESLRHWVT
EYHVDGFRFD LASVLCRGAD GTPINAPPLV KAIAKDSVLS RCKIIAEPWD CGGLYLVGKF
PNWDRWAEWN GKYRDDIRRF IKGDAGMKGN FATRIAGSAD LYRVNKRKPY HSVNFVIAHD
GFTLYDLVSY NSKHNDANGE GGNDGCNDNF SWNCGIEGET SDANINALRS QQMKNFHLAL
MVSQGTPMML MGDEYGHTRY GNNNSYGHDT AINNFQWGQL EARKNDHFRF FSKMIKFRLS
HSVLRKENFI DKSDVTWLED NWFNEESRFL AFTLHDGNGG DIYLAFNAHH FFVKTAIPSP
PRNRSWYRVV DTNLKSPDDF ITEGVAGISE TYNVAPYSAI LLEAKQ
//
