ID A0A2G2XG60_CAPBA Unreviewed; 1816 AA.
AC A0A2G2XG60;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=CQW23_04935 {ECO:0000313|EMBL:PHT56449.1};
OS Capsicum baccatum (Peruvian pepper).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum.
OX NCBI_TaxID=33114 {ECO:0000313|EMBL:PHT56449.1, ECO:0000313|Proteomes:UP000224567};
RN [1] {ECO:0000313|EMBL:PHT56449.1, ECO:0000313|Proteomes:UP000224567}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. PBC81 {ECO:0000313|Proteomes:UP000224567};
RC TISSUE=Leaf {ECO:0000313|EMBL:PHT56449.1};
RX PubMed=29089032; DOI=10.1186/s13059-017-1341-9;
RA Kim S., Park J., Yeom S.I., Kim Y.M., Seo E., Kim K.T., Kim M.S., Lee J.M.,
RA Cheong K., Shin H.S., Kim S.B., Han K., Lee J., Park M., Lee H.A.,
RA Lee H.Y., Lee Y., Oh S., Lee J.H., Choi E., Choi E., Lee S.E., Jeon J.,
RA Kim H., Choi G., Song H., Lee J., Lee S.C., Kwon J.K., Lee H.Y., Koo N.,
RA Hong Y., Kim R.W., Kang W.H., Huh J.H., Kang B.C., Yang T.J., Lee Y.H.,
RA Bennetzen J.L., Choi D.;
RT "New reference genome sequences of hot pepper reveal the massive evolution
RT of plant disease-resistance genes by retroduplication.";
RL Genome Biol. 18:R210.1-R210.11(2017).
RN [2] {ECO:0000313|Proteomes:UP000224567}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. PBC81 {ECO:0000313|Proteomes:UP000224567};
RA Kim S., Park J., Yeom S.-I., Kim Y.-M., Seo E., Kim K.-T., Kim M.-S.,
RA Lee J.M., Cheong K., Shin H.-S., Kim S.-B., Han K., Lee J., Park M.,
RA Lee H.-A., Lee H.-Y., Lee Y., Oh S., Lee J.H., Choi E., Choi E., Lee S.E.,
RA Jeon J., Kim H., Choi G., Song H., Lee J., Lee S.-C., Kwon J.-K.,
RA Lee H.-Y., Koo N., Hong Y., Kim R.W., Kang W.-H., Huh J.H., Kang B.-C.,
RA Yang T.-J., Lee Y.-H., Bennetzen J.L., Choi D.;
RT "Multiple reference genome sequences of hot pepper reveal the massive
RT evolution of plant disease resistance genes by retroduplication.";
RL J. Anim. Genet. bioRxivorg:115410-115410(2017).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PHT56449.1}.
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DR EMBL; MLFT02000002; PHT56449.1; -; Genomic_DNA.
DR STRING; 33114.A0A2G2XG60; -.
DR Proteomes; UP000224567; Chromosome 2.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:InterPro.
DR CDD; cd02584; RNAP_II_Rpb1_C; 1.
DR CDD; cd02733; RNAP_II_RPB1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.1360.140; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 2.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR000684; RNA_pol_II_repeat_euk.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR007073; RNA_pol_Rpb1_7.
DR InterPro; IPR038593; RNA_pol_Rpb1_7_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR Pfam; PF04990; RNA_pol_Rpb1_7; 1.
DR Pfam; PF05001; RNA_pol_Rpb1_R; 17.
DR PRINTS; PR01217; PRICHEXTENSN.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
DR PROSITE; PS00115; RNA_POL_II_REPEAT; 15.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000224567};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 212..518
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1508..1816
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 664..691
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1..20
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..136
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1508..1721
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1734..1803
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1816 AA; 201860 MW; 3094C9FA32CF2D45 CRC64;
MSVVHIEHGE TTERGKPKPG GLSDPRLGTI DRKMKCETCM ANMAECPGHF GHLELAKPMF
HIGFMKPVLS ILRCVCFNCS KILADEEDPK FKQAMRIRNP KNRLRKMLDA CKNKTKCEGG
DEIDVQSQDT EEPVRKSRGG CGAQQPKISI DGMKMVAEYK MQKKKSDDPE QMPEPVERKQ
QLSAERVLSI LKRVSDEDCV LLGLNPEYAR PDWMILQALP IPPPPVRPSV MMDTSSRSED
DLTHQLAMII RHNENLKRQE RNGAPAHIIS EFAQLLQFHI ATYFDNDLPG QPRATQRSGR
PIKSICSRLK SKEGRIRGNL MGKRVDFSAR TVITPDPTIN IDQLGVPWSI ALNLTYPETV
TPYNIERLKE LVEYGPHPPP GKTGAKYIIR DDGQRLDLRY LKKSSDQHLE LGYKVERHLN
DGDFVLFNRQ PSLHKMSIMG HRIKIMPYST FRLNLSVTSP YNADFDGDEM NMHVPQSFET
RAEVLELMMV PKCIVSPQAN RPVMGIVQDT LLGCRKVTKR DTFIEKDVFM NILMWWEDFD
GKVPAPAILK PRPLWTGKQV FNLIIPKQIN LLRYSAWHND SEKGYITPGD TQVRIEKGEL
LSGTLCKKTL GTSTGSLIHV IWEEVGPDAA RKFLGHTQWL VNYWLLQQAF SIGIGDTIAD
AATMEKINET ISNAKSKVKE LIKAAQEKQL EAEPGRTMME SFENRVNQVL NKARDDAGSS
AEKSLSESNN LKAMVTAGSK GSFINISQMT ACVGQQNVEG KRIPFGFIDR TLPHFTKDDY
GPESRGFVEN SYLRGLTPQE FFFHAMGGRE GLIDTAVKTS ETGYIQRRLV KAMEDIMVKY
DGTVRNSLGD VIQFLYGEDG MDSVWIETQK LDSLKAKKST FEDMYAYEID DPNWNPSYML
LEAVEDLKGI REIRSVFDAE VQKLEADRHQ LGTEIAVTGD NSWPLPVNIQ RLVLNAQKTF
KIDFRRPSDM HPMEIVEAVD KLQERLKVVP GDDYLSMEAQ KNATLFFNIL LRSALASKRV
LKEYRLSREA FEWVVGEIES RFLQSLVAPG EMIGCVAAQS IGEPATQMTL NTFHYAGVSA
KNVTLGVPRL REIINVAKKI KTPSLSVYLK PEVGKTKERA KTVQCALEYT TLRSVTQATE
VWYDPDPMST LIEEDVEFVK SYYEMPDEEI DPDKISPWLL RIELNREMMV DKKLSMADIA
EKINLEFDDD LTCIFNDDNA EKLILRIRIM NDEAPKGELD ESAEDDVFLK KIESNMLTEM
ALRGIPDINK VFIKNSKVQK FDDNEGFKAE NEWMLDTEGV NLLAVMTHED VDASRTTSNH
LIEVIEVLGI EAVRRALLDE LRVVISFDGS YVNYRHLAIL CDTMTYRGHL MAITRHGINR
NDTGPMMRCS FEETVDILLD AAVFAESDYL KGVTENIMLG QLAPIGTGGC ALYLNEEMLK
QAIEIPLPSY MEGGLEFGMT PGRSPISGTP YHDGMMSPNY LLSPNMRMSP MTDAQFSPYV
GGMAFSPTSS PGYSPSSPGY SPSSPGYSPT SPGYSPTSPG YSPTSPGYSP TSPTYSPSSP
GYSPSSPAYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPA YSPTSPAYSP
TSPAYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPTY SPTSPSYSPT SPAYSPTSPG
YSPTSPSYSP TSPSYSPTSP SYNPSARYSP SLAYSPTSPK LSPSSPYSPS SPSYSPTSPS
YSPTSPSYSP SSPTYSPSSP FNNSGTSPDY SPSSPQYSPS AGYSPSAPGY SPSSTSQYTP
RISDRDNKSV KDDKTG
//
