ID A0A2G2Y2J3_CAPAN Unreviewed; 311 AA.
AC A0A2G2Y2J3;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=16S rRNA (uracil(1498)-N(3))-methyltransferase {ECO:0000256|ARBA:ARBA00012328};
DE EC=2.1.1.193 {ECO:0000256|ARBA:ARBA00012328};
GN ORFNames=T459_32252 {ECO:0000313|EMBL:PHT63954.1};
OS Capsicum annuum (Capsicum pepper).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum.
OX NCBI_TaxID=4072 {ECO:0000313|EMBL:PHT63954.1, ECO:0000313|Proteomes:UP000222542};
RN [1] {ECO:0000313|EMBL:PHT63954.1, ECO:0000313|Proteomes:UP000222542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. CM334 {ECO:0000313|Proteomes:UP000222542};
RX PubMed=29089032; DOI=10.1186/s13059-017-1341-9;
RA Kim S., Park J., Yeom S.I., Kim Y.M., Seo E., Kim K.T., Kim M.S., Lee J.M.,
RA Cheong K., Shin H.S., Kim S.B., Han K., Lee J., Park M., Lee H.A.,
RA Lee H.Y., Lee Y., Oh S., Lee J.H., Choi E., Choi E., Lee S.E., Jeon J.,
RA Kim H., Choi G., Song H., Lee J., Lee S.C., Kwon J.K., Lee H.Y., Koo N.,
RA Hong Y., Kim R.W., Kang W.H., Huh J.H., Kang B.C., Yang T.J., Lee Y.H.,
RA Bennetzen J.L., Choi D.;
RT "New reference genome sequences of hot pepper reveal the massive evolution
RT of plant disease-resistance genes by retroduplication.";
RL Genome Biol. 18:R210.1-R210.11(2017).
CC -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC ribosomal subunit. {ECO:0000256|ARBA:ARBA00025699}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC Evidence={ECO:0000256|ARBA:ARBA00000792};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC {ECO:0000256|ARBA:ARBA00005528}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PHT63954.1}.
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DR EMBL; AYRZ02000021; PHT63954.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G2Y2J3; -.
DR STRING; 4072.A0A2G2Y2J3; -.
DR EnsemblPlants; PHT63954; PHT63954; T459_32252.
DR Gramene; PHT63954; PHT63954; T459_32252.
DR OMA; RCITQWK; -.
DR Proteomes; UP000222542; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070042; F:rRNA (uridine-N3-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR CDD; cd18084; RsmE-like; 1.
DR Gene3D; 3.40.1280.10; -; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR006700; RsmE.
DR InterPro; IPR046886; RsmE_MTase_dom.
DR InterPro; IPR046887; RsmE_PUA-like.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR NCBIfam; TIGR00046; RsmE family RNA methyltransferase; 1.
DR PANTHER; PTHR30027:SF3; 16S RRNA (URACIL(1498)-N(3))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR30027; RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE E; 1.
DR Pfam; PF04452; Methyltrans_RNA; 1.
DR Pfam; PF20260; PUA_4; 1.
DR SUPFAM; SSF75217; alpha/beta knot; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000222542};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 85..128
FT /note="Ribosomal RNA small subunit methyltransferase E PUA-
FT like"
FT /evidence="ECO:0000259|Pfam:PF20260"
FT DOMAIN 144..300
FT /note="Ribosomal RNA small subunit methyltransferase E
FT methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04452"
SQ SEQUENCE 311 AA; 34296 MW; FE1F65CEBE0EF00F CRC64;
MQASVWSPRL VNLFTRSCKT TNSLRAFSSS AISNQSRGGL PRFYSDELPP SKASPLLPTF
RKEQCWGLLY YQFESARNDG VVRVKGDEFW HMTRVLRLTV HDRVELFDGK GGLVEGCIQI
IDQTGVDIVA LENPKSVSPH NTQWHVYAAF GTLKGGRADW LVEKCTELGA CSVTPLLTER
SPSISENRVD RLQRVSFAAA KQCQRLHEMV LNPPIKVGGL LTLVKNSKLS FIAIAEAKPV
FSALSSTKRE SAGLMIIGPE GDFTERELNV ILEAGATAVG LGPHRLRVET ATVALLSALM
LWCDDQEILK V
//
