ID A0A2G4EVS9_9CYAN Unreviewed; 315 AA.
AC A0A2G4EVS9;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Probable aspartoacylase {ECO:0000256|HAMAP-Rule:MF_00704};
DE EC=3.5.1.15 {ECO:0000256|HAMAP-Rule:MF_00704};
GN ORFNames=CP500_020435 {ECO:0000313|EMBL:PHX53634.1};
OS Tychonema bourrellyi FEM_GT703.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Oscillatoriales; Microcoleaceae; Tychonema.
OX NCBI_TaxID=2040638 {ECO:0000313|EMBL:PHX53634.1, ECO:0000313|Proteomes:UP000226442};
RN [1] {ECO:0000313|EMBL:PHX53634.1, ECO:0000313|Proteomes:UP000226442}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FEM_GT703 {ECO:0000313|EMBL:PHX53634.1,
RC ECO:0000313|Proteomes:UP000226442};
RA Banno H., Chua N.-H.;
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:PHX53634.1, ECO:0000313|Proteomes:UP000226442}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FEM_GT703 {ECO:0000313|EMBL:PHX53634.1,
RC ECO:0000313|Proteomes:UP000226442};
RA Tett A., Armanini F., Asnicar F., Boscaini A., Pasolli E., Zolfo M.,
RA Donati C., Salmaso N., Segata N.;
RT "Draft genome sequence of the planktic cyanobacteria Tychonema bourrellyi
RT isolated from alpine lentic freshwater.";
RL Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N-acyl-L-aspartate = a carboxylate + L-aspartate;
CC Xref=Rhea:RHEA:10872, ChEBI:CHEBI:15377, ChEBI:CHEBI:29067,
CC ChEBI:CHEBI:29991, ChEBI:CHEBI:58497; EC=3.5.1.15;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00704};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00704,
CC ECO:0000256|PIRSR:PIRSR018001-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00704,
CC ECO:0000256|PIRSR:PIRSR018001-3};
CC -!- SIMILARITY: Belongs to the AspA/AstE family. Aspartoacylase subfamily.
CC {ECO:0000256|ARBA:ARBA00006173, ECO:0000256|HAMAP-Rule:MF_00704}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PHX53634.1}.
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DR EMBL; NXIB02000169; PHX53634.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G4EVS9; -.
DR OrthoDB; 531770at2; -.
DR Proteomes; UP000226442; Unassembled WGS sequence.
DR GO; GO:0019807; F:aspartoacylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR CDD; cd06909; M14_ASPA; 1.
DR Gene3D; 2.20.25.160; -; 1.
DR Gene3D; 3.40.630.10; Zn peptidases; 1.
DR HAMAP; MF_00704; Aspartoacylase; 1.
DR InterPro; IPR016708; Aspartoacylase.
DR InterPro; IPR007036; Aste_AspA.
DR PANTHER; PTHR15162; ASPARTOACYLASE; 1.
DR PANTHER; PTHR15162:SF7; SUCCINYLGLUTAMATE DESUCCINYLASE; 1.
DR Pfam; PF04952; AstE_AspA; 1.
DR PIRSF; PIRSF018001; Aspartoacylase; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00704};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_00704}; Reference proteome {ECO:0000313|Proteomes:UP000226442};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|HAMAP-Rule:MF_00704}.
FT ACT_SITE 165
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR018001-1"
FT BINDING 14
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00704,
FT ECO:0000256|PIRSR:PIRSR018001-3"
FT BINDING 17
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00704,
FT ECO:0000256|PIRSR:PIRSR018001-3"
FT BINDING 56
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00704"
FT BINDING 63..64
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00704"
FT BINDING 106
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00704,
FT ECO:0000256|PIRSR:PIRSR018001-3"
FT BINDING 165
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00704"
FT BINDING 276
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00704"
SQ SEQUENCE 315 AA; 35340 MW; 6B0677F9C43C8965 CRC64;
MNKIKRVAII GGTHGNELIG IYLVNKFDRQ PDLIARSTFQ TMALLANPKA CAIGKRYIDI
DLNRCFLQQD LENPNLSSYE SERAKEIYHI FSSKNRDDAD LIIDLHTTTS NMGLTFILDS
QHPFDLQLAT HLTSVYPNFK VLASGTQNQD TSVLRSISKL GFTLEVGPVA QGVLDASLFQ
QTEALVGTIL DWVEAYNQET IAPVENPLTI YQKVQTIDYP RNESGEIQAM IHPQLQFRDY
EPLNPGDPMF LTFDGESLFY EGKSTVYPVF INEAAYYEKG ISMYLTQKQI VIGNGEWGIG
NGELGISNWE LVIGN
//
