ID A0A2G5C191_AQUCA Unreviewed; 339 AA.
AC A0A2G5C191;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein {ECO:0000259|Pfam:PF01073};
GN ORFNames=AQUCO_13300009v1 {ECO:0000313|EMBL:PIA25015.1};
OS Aquilegia coerulea (Rocky mountain columbine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Thalictroideae;
OC Aquilegia.
OX NCBI_TaxID=218851 {ECO:0000313|EMBL:PIA25015.1, ECO:0000313|Proteomes:UP000230069};
RN [1] {ECO:0000313|EMBL:PIA25015.1, ECO:0000313|Proteomes:UP000230069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Goldsmith {ECO:0000313|Proteomes:UP000230069};
RA Hodges S., Kramer E., Nordborg M., Tomkins J., Borevitz J., Derieg N.,
RA Yan J., Mihaltcheva S., Hayes R.D., Rokhsar D.;
RT "WGS assembly of Aquilegia coerulea Goldsmith.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the 3-beta-HSD family.
CC {ECO:0000256|RuleBase:RU004475}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU004475}.
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DR EMBL; KZ305147; PIA25015.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G5C191; -.
DR Proteomes; UP000230069; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:InterPro.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:InterPro.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR028110; TMEM254.
DR Pfam; PF01073; 3Beta_HSD; 1.
DR Pfam; PF14934; TMEM254; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|RuleBase:RU004475};
KW Oxidoreductase {ECO:0000256|RuleBase:RU004475};
KW Reference proteome {ECO:0000313|Proteomes:UP000230069};
KW Transmembrane {ECO:0000256|RuleBase:RU004475};
KW Transmembrane helix {ECO:0000256|RuleBase:RU004475}.
FT TRANSMEM 6..27
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU004475"
FT TRANSMEM 158..176
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU004475"
FT TRANSMEM 241..263
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU004475"
FT TRANSMEM 275..296
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU004475"
FT TRANSMEM 316..332
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU004475"
FT DOMAIN 31..151
FT /note="3-beta hydroxysteroid dehydrogenase/isomerase"
FT /evidence="ECO:0000259|Pfam:PF01073"
SQ SEQUENCE 339 AA; 38480 MW; 471D8F2E2DA753D9 CRC64;
MKPCLIFLLM TTLIHMVGVN LLLNSWFSRA MVANPSRKKN GTCLYTCVIR PAAIYGPGEE
RHLPRIVSLA KLGLFTFRIG DAGVKTDWVY LDNLVLALIL ASMGLLDDIP GREGRPVAAG
QPYFICDGSP INTFEFLRPL LKSLEYDLPN STLDVSHALF LGQMFWALYT FFYPWLNRSW
LPQPFILPAE VYKVGVTHYF SYLKAKEELG YVPVVSPQEG MSTTISYWQD RNRKSLDGPT
LYAWLFCIIG MTALFCAAFL PAYGPNKFNR IVTVFFLRSI TTAQIVFVLA AMAHVIEGLY
AWKLAKRVDP LNAKGWFWQT FALGFFSLRL LLKRAKKQD
//
