ID   A0A2G5CST4_AQUCA        Unreviewed;       724 AA.
AC   A0A2G5CST4;
DT   31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT   31-JAN-2018, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE            EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN   ORFNames=AQUCO_03900042v1 {ECO:0000313|EMBL:PIA33917.1};
OS   Aquilegia coerulea (Rocky mountain columbine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Thalictroideae;
OC   Aquilegia.
OX   NCBI_TaxID=218851 {ECO:0000313|EMBL:PIA33917.1, ECO:0000313|Proteomes:UP000230069};
RN   [1] {ECO:0000313|EMBL:PIA33917.1, ECO:0000313|Proteomes:UP000230069}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Goldsmith {ECO:0000313|Proteomes:UP000230069};
RA   Hodges S., Kramer E., Nordborg M., Tomkins J., Borevitz J., Derieg N.,
RA   Yan J., Mihaltcheva S., Hayes R.D., Rokhsar D.;
RT   "WGS assembly of Aquilegia coerulea Goldsmith.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|ARBA:ARBA00004906}.
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DR   EMBL; KZ305056; PIA33917.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2G5CST4; -.
DR   STRING; 218851.A0A2G5CST4; -.
DR   InParanoid; A0A2G5CST4; -.
DR   OrthoDB; 5489991at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000230069; Unassembled WGS sequence.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   CDD; cd16664; RING-Ubox_PUB; 1.
DR   Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR045210; RING-Ubox_PUB.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR23315; U BOX DOMAIN-CONTAINING; 1.
DR   PANTHER; PTHR23315:SF266; U-BOX DOMAIN-CONTAINING PROTEIN 17; 1.
DR   Pfam; PF00514; Arm; 1.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00185; ARM; 4.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   SUPFAM; SSF57850; RING/U-box; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 2.
DR   PROSITE; PS51698; U_BOX; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000230069}.
FT   DOMAIN          292..366
FT                   /note="U-box"
FT                   /evidence="ECO:0000259|PROSITE:PS51698"
FT   REPEAT          437..479
FT                   /note="ARM"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00259"
FT   REPEAT          522..564
FT                   /note="ARM"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00259"
SQ   SEQUENCE   724 AA;  79537 MW;  2F57611EAB8AF1FF CRC64;
     MASAAIFSSL RRRRSPSLEA FLAPVYLNEI TLVQTLASIS GELVSTFSNH SFSLQRKNSR
     SLIRKIEIFV VLFEYLRDSS IKVPSSAMLC FKEFYLLVYR SKILLDYCLQ SSRLWLLLQN
     SSISGHFHDL NQEISTLLDV FPLKEIDVSQ DVYEQIELLK KQSRRAKLFI DQRDEALRIQ
     LFSFLDEFEK GRIPNRLELR VFLIEKLGIR DVKICRAEIE FLEEQIYNHE GDVEPTVSVL
     NGFVALTRYC RFLLFGFKEE EEEAKGGETS FGNRKKPRKG LISQEIGDTF LTVPKDFCCP
     ISLDLMQDPV IISTGQTYDR YSIARWMEEG HSSCPKTGQM LAHTRLVPNR ALRNLISQWC
     TAHGIPFDAL ENTDMSMEVV ALAASTKAAI EANKATAELL IQQLSNGSQG AKTVAARELR
     LLAKTGKENR ACIAEAGAIP LLHKLLPSPN PIAQENSVTA ILNLSIHDKN KSRIMEEAGC
     LGAIVEVLRF GLTTEARENA AATLFSLSAV HDYKKRISEE TGAVEALVGL LREGSSRGKK
     DAVTALFNLS THVDNCTRMI ESGAVAALVG ELGTEGVAEE AAGALALLVR QPIGAEAVGK
     EEMAVTGLLG MMRRGTPRGK ENAVAALLEL CRSGGTATTQ RVVRAPALAS LLQTLLFTGT
     KRARRKAASL ARVCQRCQAA TLPMGSWGVG YTLGYTLTGN STTNHSSSFA GDVTVPVSIS
     VPVL
//