ID A0A2G5CTD5_AQUCA Unreviewed; 619 AA.
AC A0A2G5CTD5;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Carotenoid cleavage dioxygenase 7 {ECO:0008006|Google:ProtNLM};
GN ORFNames=AQUCO_03700073v1 {ECO:0000313|EMBL:PIA34538.1};
OS Aquilegia coerulea (Rocky mountain columbine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Thalictroideae;
OC Aquilegia.
OX NCBI_TaxID=218851 {ECO:0000313|EMBL:PIA34538.1, ECO:0000313|Proteomes:UP000230069};
RN [1] {ECO:0000313|EMBL:PIA34538.1, ECO:0000313|Proteomes:UP000230069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Goldsmith {ECO:0000313|Proteomes:UP000230069};
RA Hodges S., Kramer E., Nordborg M., Tomkins J., Borevitz J., Derieg N.,
RA Yan J., Mihaltcheva S., Hayes R.D., Rokhsar D.;
RT "WGS assembly of Aquilegia coerulea Goldsmith.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787}.
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DR EMBL; KZ305054; PIA34538.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G5CTD5; -.
DR STRING; 218851.A0A2G5CTD5; -.
DR InParanoid; A0A2G5CTD5; -.
DR OrthoDB; 34984at2759; -.
DR Proteomes; UP000230069; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR PANTHER; PTHR10543:SF37; CAROTENOID CLEAVAGE DIOXYGENASE 7, CHLOROPLASTIC; 1.
DR Pfam; PF03055; RPE65; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00022964};
KW Reference proteome {ECO:0000313|Proteomes:UP000230069}.
FT BINDING 239
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 325
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 403
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 612
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 619 AA; 69417 MW; 7E63A531E56849D3 CRC64;
MEATTSSHFF PSKIPSLPRT LLSAQSLKPP TTIRCAVSIT TTPEPQLPLT TTTTADDSIT
AFWDYQFLFV SQRSETTKPL TLRVIEGSIP SDFPRGTYYL TGPGIFTDDH GSTVHPLDGH
GYLRAFEING SKGEVNFSAK YIKTEAQMEE CDSETGRWQF KYRGPFSVLR AGKKLGNVKV
MKNVANTSVV KWGGRLLCLW EGGVPYEIES KTLDTVGRFD VIDGEDCVLK SGINGGVSHS
LSQGGVWDVA ARLLKPILHG VYNMPAKRLL SHYKIDPHRE RLLLVSCNAE DMLLPQSTFT
FYEFDSNYKL MNKQTFNIPE HMMIHDWAFT DSHYILFGNR IKLDIPGSML ALSGISPMIS
ALSVDSSKPT SSIYLLPRFP DEVVDNRDWR IPIEAPKQMW LLHVGNAFEQ RDINGNMEIQ
IHAAACSYQW FNFCKMFGYN WKSGKLDPAF MNAREGEEDS LPHLVEVSMN FDREGKCQNC
SVEPLNFWTT PSDFPVINPV VSSRKNTYIY AATSSGSCQT LPHFPFDTVA KLNVQNKSVS
TWTVGSRRFI GEPIFIAKGD TEDDGYLLVV EYAVSIQRCY LVILDPKRIG EADAVIAKLE
VPKSFNFALG FHGFWAGED
//
