ID   A0A2G5CTQ1_AQUCA        Unreviewed;       810 AA.
AC   A0A2G5CTQ1;
DT   31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT   31-JAN-2018, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   RecName: Full=pyruvate kinase {ECO:0000256|ARBA:ARBA00012142};
DE            EC=2.7.1.40 {ECO:0000256|ARBA:ARBA00012142};
GN   ORFNames=AQUCO_03700139v1 {ECO:0000313|EMBL:PIA34648.1};
OS   Aquilegia coerulea (Rocky mountain columbine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Thalictroideae;
OC   Aquilegia.
OX   NCBI_TaxID=218851 {ECO:0000313|EMBL:PIA34648.1, ECO:0000313|Proteomes:UP000230069};
RN   [1] {ECO:0000313|EMBL:PIA34648.1, ECO:0000313|Proteomes:UP000230069}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Goldsmith {ECO:0000313|Proteomes:UP000230069};
RA   Hodges S., Kramer E., Nordborg M., Tomkins J., Borevitz J., Derieg N.,
RA   Yan J., Mihaltcheva S., Hayes R.D., Rokhsar D.;
RT   "WGS assembly of Aquilegia coerulea Goldsmith.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=K(+); Xref=ChEBI:CHEBI:29103;
CC         Evidence={ECO:0000256|ARBA:ARBA00001958};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 5/5. {ECO:0000256|ARBA:ARBA00004997}.
CC   -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC       {ECO:0000256|ARBA:ARBA00008663}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KZ305054; PIA34648.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2G5CTQ1; -.
DR   STRING; 218851.A0A2G5CTQ1; -.
DR   InParanoid; A0A2G5CTQ1; -.
DR   OrthoDB; 454163at2759; -.
DR   UniPathway; UPA00109; UER00188.
DR   Proteomes; UP000230069; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR   GO; GO:0004743; F:pyruvate kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 2.
DR   Gene3D; 2.40.33.10; PK beta-barrel domain-like; 1.
DR   InterPro; IPR001697; Pyr_Knase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR   InterPro; IPR011037; Pyrv_Knase-like_insert_dom_sf.
DR   InterPro; IPR015793; Pyrv_Knase_brl.
DR   InterPro; IPR015806; Pyrv_Knase_insert_dom_sf.
DR   PANTHER; PTHR11817:SF76; PLASTIDIAL PYRUVATE KINASE 4, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR11817; PYRUVATE KINASE; 1.
DR   Pfam; PF00224; PK; 2.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   SUPFAM; SSF50800; PK beta-barrel domain-like; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Pyruvate {ECO:0000256|ARBA:ARBA00023317};
KW   Reference proteome {ECO:0000313|Proteomes:UP000230069};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          324..410
FT                   /note="Pyruvate kinase barrel"
FT                   /evidence="ECO:0000259|Pfam:PF00224"
FT   DOMAIN          527..773
FT                   /note="Pyruvate kinase barrel"
FT                   /evidence="ECO:0000259|Pfam:PF00224"
SQ   SEQUENCE   810 AA;  89579 MW;  9187E17A27A9B680 CRC64;
     MQFRFFLSIS KVESITTKSV HNFLQFFAIF YVFFRQIAKQ MTLHAPSAHF VAEQCSIYNH
     VSQIANPSVL NNFKSGKISR PTCRFYIRSS VFGYGLIQLQ TKPNANLRKK CFIFASLKEN
     DEERGSSGPY IEEEMPQSIF KVSETNQNSN DSDVETAASV PISEDNLIKD ATDNMLSQGN
     LLDKLKAVHL HVLALEQWNA SRLKLCHRGY YASATNLIHY LALKCLDVGQ LKEELSSNGL
     LNLDSVNNYV LASITAAVNM LENQSYSINS KANGTSTIFN FDVLNKSTWD HSNQRDNVDF
     SINTLKKRVF SHSEALLGPL RDERTTHVMV TVGREATEND TLLSDLLRAG ATLFRINCAY
     GDPSVWSEII RRAKKSSQML EKPCRILMDL AGPKLRTGSM EVGPSVLKIS PKKNASGYVI
     FPAQVWLSQP GSGPPPAHLS MDANLVIDNE ELLHRLEVGN VLSFYDTRGK RRLLKITKKV
     PVFGGIGCVA ECSRTAYVES GTTMCMKGKK RRFATGNVVE VPCTEQFVRL KTGDLLTISR
     DPCLTPEHRS GESATNAPRI TCSSSHLFDS VKPGEPIAFD DGRIWGVIQG TSISEIIVSI
     TCASPKGSKL GSGKSINIPE SEIKFEGLTS KDLMDLEFVA SHADMVGISF VRDVRDIVVL
     QNELEKRKLQ NLGVVLKIET RSGFEKLPLL LLQAMRSRNP LGIMIARGDL AVECGWERLA
     DIQEEIMSIC GAAHVPVIWA TQVLESLVKS GLPTRAEISD VASGKRASCI MLNKGKFITE
     AVSTLDSMLR VNTEKVKAEL KPLVLSSHTS
//