ID   A0A2G5DC36_AQUCA        Unreviewed;       404 AA.
AC   A0A2G5DC36;
DT   31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT   31-JAN-2018, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE            EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN   ORFNames=AQUCO_02300122v1 {ECO:0000313|EMBL:PIA41098.1};
OS   Aquilegia coerulea (Rocky mountain columbine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Thalictroideae;
OC   Aquilegia.
OX   NCBI_TaxID=218851 {ECO:0000313|EMBL:PIA41098.1, ECO:0000313|Proteomes:UP000230069};
RN   [1] {ECO:0000313|EMBL:PIA41098.1, ECO:0000313|Proteomes:UP000230069}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Goldsmith {ECO:0000313|Proteomes:UP000230069};
RA   Hodges S., Kramer E., Nordborg M., Tomkins J., Borevitz J., Derieg N.,
RA   Yan J., Mihaltcheva S., Hayes R.D., Rokhsar D.;
RT   "WGS assembly of Aquilegia coerulea Goldsmith.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC         mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC         Evidence={ECO:0000256|ARBA:ARBA00001678};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
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DR   EMBL; KZ305040; PIA41098.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2G5DC36; -.
DR   STRING; 218851.A0A2G5DC36; -.
DR   InParanoid; A0A2G5DC36; -.
DR   OrthoDB; 2717493at2759; -.
DR   Proteomes; UP000230069; Unassembled WGS sequence.
DR   GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR045053; MAN-like.
DR   PANTHER; PTHR31451:SF53; MANNAN ENDO-1,4-BETA-MANNOSIDASE; 1.
DR   PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW   Hydrolase {ECO:0000256|RuleBase:RU361153};
KW   Reference proteome {ECO:0000313|Proteomes:UP000230069};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           24..404
FT                   /note="mannan endo-1,4-beta-mannosidase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5013928980"
FT   DOMAIN          42..338
FT                   /note="Glycoside hydrolase family 5"
FT                   /evidence="ECO:0000259|Pfam:PF00150"
SQ   SEQUENCE   404 AA;  45611 MW;  B4E3B2F12854FDCC CRC64;
     MSCLCRLSWI LGIFILLQVV CEARVPRAMS FVATSNNQFV LNGAPFLFNG FNSYWMMNVA
     VDPSERHKVS EVFRDASAIG LTVCRTWAFS DGGDKALQIS PGVYDERVFQ ALDFVVSEAR
     QYGIRLVLSF VNNYNDFGGK AQYVQWAKNA GVPINSDDDF YTNDVLKKYY KDHVQKVVTR
     LNTITGMAYK DDSTIMAWEL MNEPRCQEDY SGKTVNDWAQ EMATFVKSID SQHLLEIGME
     GFYGDSMPDK KQFNPGYQVG TDFISSNQIK EIDFATIHAY PDLWLPGQGE DSQLAFVQRW
     ISSHWTDAQT TIRKPFVFAE FGKSKKDPGY SLSARDSYIN NIYSNIYNDA RNGETFAGGM
     VWQIMAQGME SYYDGYEIVL SQDPSTAGLI GAQSQKMTAL ARSL
//