ID A0A2G5E4B5_AQUCA Unreviewed; 495 AA.
AC A0A2G5E4B5;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=AQUCO_01200041v1 {ECO:0000313|EMBL:PIA50576.1};
OS Aquilegia coerulea (Rocky mountain columbine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Thalictroideae;
OC Aquilegia.
OX NCBI_TaxID=218851 {ECO:0000313|EMBL:PIA50576.1, ECO:0000313|Proteomes:UP000230069};
RN [1] {ECO:0000313|EMBL:PIA50576.1, ECO:0000313|Proteomes:UP000230069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Goldsmith {ECO:0000313|Proteomes:UP000230069};
RA Hodges S., Kramer E., Nordborg M., Tomkins J., Borevitz J., Derieg N.,
RA Yan J., Mihaltcheva S., Hayes R.D., Rokhsar D.;
RT "WGS assembly of Aquilegia coerulea Goldsmith.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
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DR EMBL; KZ305029; PIA50576.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G5E4B5; -.
DR InParanoid; A0A2G5E4B5; -.
DR OrthoDB; 692557at2759; -.
DR Proteomes; UP000230069; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR10492:SF57; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR10492; UNCHARACTERIZED; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000230069}.
FT DOMAIN 338..382
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
SQ SEQUENCE 495 AA; 55347 MW; 4870F06C8D143527 CRC64;
MPMPVKDWNF ETSNRLLWEH QKLMHDGLQA EAERDFSKLN SDQKHAYTVI IDSVLNDTGK
VFFLNGPAGT GKTFVYNTIA AKIRSEGQIV VMVASSGIAS LLLTGGRTTH STFKIPFDVM
DDTVCGFKKN SNHAELFRKT KLIVWDEVPM QHRFCIEAVD RSLQDIMENN KPFGGITVVL
GGDFKQTLPV VPKGTREQIV GASLRNSNLW SQVEVLSLVK NMRLDSSDPE NIKFAEYLIK
VGTNPEEKVQ VPENFNRCKD TMELISKVYP DLNIKEKLTQ QYLEERSILS ARNDDVSAIN
GSAINLFPGE LYEFLAAGKV IEEDIEVENR GNQIASENLN SLDPPSLPPF NLQLKIGCPI
MLLRNLQPRD GLCNGTRLMV VNCGTRVIEA KILNGSHAGD LVFIPRISLI PTVTETPFPM
ARRQFPVRLA FAMTINKSQG QSVRYVGIDL RNPVFSHGQL YVAFSRCTSS DRISVLLPND
DDNTTTNVVY PEVLL
//
