ID   A0A2G5E5A0_AQUCA        Unreviewed;      1035 AA.
AC   A0A2G5E5A0;
DT   31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT   31-JAN-2018, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE            EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN   ORFNames=AQUCO_01100021v1 {ECO:0000313|EMBL:PIA50920.1};
OS   Aquilegia coerulea (Rocky mountain columbine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Thalictroideae;
OC   Aquilegia.
OX   NCBI_TaxID=218851 {ECO:0000313|EMBL:PIA50920.1, ECO:0000313|Proteomes:UP000230069};
RN   [1] {ECO:0000313|EMBL:PIA50920.1, ECO:0000313|Proteomes:UP000230069}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Goldsmith {ECO:0000313|Proteomes:UP000230069};
RA   Hodges S., Kramer E., Nordborg M., Tomkins J., Borevitz J., Derieg N.,
RA   Yan J., Mihaltcheva S., Hayes R.D., Rokhsar D.;
RT   "WGS assembly of Aquilegia coerulea Goldsmith.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000256|RuleBase:RU363044};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|RuleBase:RU363044};
CC   -!- SIMILARITY: Belongs to the helicase family.
CC       {ECO:0000256|RuleBase:RU363044}.
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DR   EMBL; KZ305028; PIA50920.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2G5E5A0; -.
DR   STRING; 218851.A0A2G5E5A0; -.
DR   InParanoid; A0A2G5E5A0; -.
DR   Proteomes; UP000230069; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR010285; DNA_helicase_pif1-like.
DR   InterPro; IPR025476; Helitron_helicase-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR049163; Pif1-like_2B_dom.
DR   PANTHER; PTHR45786:SF82; ATP-DEPENDENT DNA HELICASE; 1.
DR   PANTHER; PTHR45786; DNA BINDING PROTEIN-LIKE; 1.
DR   Pfam; PF14214; Helitron_like_N; 1.
DR   Pfam; PF05970; PIF1; 1.
DR   Pfam; PF21530; Pif1_2B_dom; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|RuleBase:RU363044};
KW   DNA damage {ECO:0000256|RuleBase:RU363044};
KW   DNA recombination {ECO:0000256|RuleBase:RU363044};
KW   DNA repair {ECO:0000256|RuleBase:RU363044};
KW   Helicase {ECO:0000256|RuleBase:RU363044};
KW   Hydrolase {ECO:0000256|RuleBase:RU363044};
KW   Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW   Reference proteome {ECO:0000313|Proteomes:UP000230069}.
FT   DOMAIN          317..419
FT                   /note="Helitron helicase-like"
FT                   /evidence="ECO:0000259|Pfam:PF14214"
FT   DOMAIN          903..945
FT                   /note="DNA helicase Pif1-like 2B"
FT                   /evidence="ECO:0000259|Pfam:PF21530"
SQ   SEQUENCE   1035 AA;  118174 MW;  E4170FA1A4B35271 CRC64;
     MEGNYCTTVV HPVSDSHYDT ILEDEIHIPI DPVENVPKER HYLGKMDVIC PSCFAYHWLD
     ERLTKSTKKK SFFGMCCLQG KIKLSLLTPL PSAIKLLYEG RNSLARSFRK DIRFYNAANA
     FSSLGVSMDK RILKGRGPTS FTIHGQLSHQ IGSLLPESNK SPSYSQLYIY DPHIALECRQ
     KRNLHLNPAI LKIIQEARLE SNELCKIYKC VYDVLKSANS NGGESIPIRL AYTPTTDPRR
     YNLPTSEDIA VIIPGDETEP TSKRDIILHL KNNNLERISE CHPLYLPSHY VLLFPTGDLG
     WSTDMKHWDV KNEQYFTVIG SPRHMYEIYQ DSMAITRYYK HPDIFMTVTS NPKWPEIQNE
     LKPGQAALDR PDLVARVFEL KRKAIMHEIE NKRLFGRVVA NVYTIEFQKR GLPHMHTLLF
     LHPEDKIRTT DQVDRIVSAE FPDPIKHPAL FETIKKCMVH GPCGTRNMES VCMENKVCKS
     RYPRENVNDT RIDVDGYPIY RRRKIGKSYH VRGHLVDNKD VVPYNPHLSE MLIATLMWKF
     VHHYKHCCIY SYSVASEHVF QVHRATLRSR YPDRQLNPIV VAVKVTHPDP SDSDVCDYGL
     FLMNDYLLES EITNSVRNDS RQVFFLSGPA GTGKTFVYNT VADTLRSEGH IVIMVASSGI
     ASLLLTGGRT AHSTFKIPFE VLDDSVCSVT KQTIHAELFK RAKLIIWDEV PMQHRFCVEA
     DDRTLRDIRE IKEPFGGVTV VLGDDFKQTL HVITKGTRQE IVRACLTKSH LWSGVRLLTL
     VKNMRLNNND TENVKFADYL IEIGTNPKED VELPSEIKMC KSTIDLLFKV YPNLNVEEVA
     TETYLQERSI LSARNDDVAT LNELAINHFP GELHEYLAAD KAIEDDQPIE NRGNSISNEN
     MQALDPPSLP PFKLQLKVVC PIMLLRNLQP RDGLCNGTRL MVVQFATRVI EAKILNGSHV
     GNYVFIPRIT LQPSVSETPF QMARRQFPVR LAFAMTINKS QGQSVKCTSS NRIFVLLGNE
     DDDKTTNVVY PEVLL
//