ID A0A2G5KZ30_9BACT Unreviewed; 534 AA.
AC A0A2G5KZ30;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=Ricin B lectin domain-containing protein {ECO:0000259|SMART:SM00458};
GN ORFNames=BFP72_04375 {ECO:0000313|EMBL:PIB34697.1};
OS Reichenbachiella sp. 5M10.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Reichenbachiellaceae;
OC Reichenbachiella.
OX NCBI_TaxID=1889772 {ECO:0000313|EMBL:PIB34697.1, ECO:0000313|Proteomes:UP000229466};
RN [1] {ECO:0000313|EMBL:PIB34697.1, ECO:0000313|Proteomes:UP000229466}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=5M10 {ECO:0000313|EMBL:PIB34697.1,
RC ECO:0000313|Proteomes:UP000229466};
RA Wong S.-K., Hamasaki K., Yoshizawa S.;
RT "Draft genome of Reichenbachiella sp. strain 5M10.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PIB34697.1}.
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DR EMBL; MDGR01000007; PIB34697.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G5KZ30; -.
DR Proteomes; UP000229466; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd08998; GH43_Arb43a-like; 1.
DR CDD; cd00161; RICIN; 1.
DR Gene3D; 2.80.10.50; -; 2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR InterPro; IPR026444; Secre_tail.
DR NCBIfam; TIGR04183; Por_Secre_tail; 1.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR Pfam; PF18962; Por_Secre_tail; 1.
DR Pfam; PF14200; RicinB_lectin_2; 2.
DR SMART; SM00458; RICIN; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000229466};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..534
FT /note="Ricin B lectin domain-containing protein"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5013808039"
FT DOMAIN 296..435
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
FT ACT_SITE 27
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 187
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 141
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 534 AA; 58309 MW; C542F3F71152A673 CRC64;
MYFGGMLLFS LLWITDVTQA QYAPSHDPST MIRNSDGRYW IFTTGDGVWA MSSSNADFTD
WRPEPTPFAI GTWPGWISNY VSGFNGFFWA PDVIKVGNTY FLYYSCAGNG APAAIGVATA
TNLAGPWTDQ GMVVAGNNAI DPALLLDNGR LWMSWGNWQT GIDIIELSTA TGKPISSSTH
LVSGQVEGPG LMKNGGYYYL YYQRGLCCGG VNSSYYMVVA RATSITGPYT NERVFLPNQN
GNIIGPGHFG YGEGRLTYHF YDGNDNGNAK LMTRSDFGYS NGWPYVGAPP SPVVSGGTYR
IAPRHSGKTV DVVNCGNTDG TNVNQWSWLN NNCQKWIFTD AGNGTWRISP ASASGLALDV
ANCSADNLAN VQLWSWLNND CQKWHLVDRG GGYYSLASAA SGKCLDVNGV SSSDGANMIQ
YTCYSNSYNQ QFSLTPVNIG ARTADDIENK SLADASLVQT YPNPAQDFIE IKMPGNKELT
TSLMLYNTAG ALVKHEDFVD HSYSLDVSDL ENGLYVIVLQ NDEQKLNRKI QILR
//
