ID   A0A2G8JN16_STIJA        Unreviewed;       534 AA.
AC   A0A2G8JN16;
DT   31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT   31-JAN-2018, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE            EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN   ORFNames=BSL78_26041 {ECO:0000313|EMBL:PIK37127.1};
OS   Stichopus japonicus (Sea cucumber).
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Holothuroidea;
OC   Aspidochirotacea; Aspidochirotida; Stichopodidae; Apostichopus.
OX   NCBI_TaxID=307972 {ECO:0000313|EMBL:PIK37127.1, ECO:0000313|Proteomes:UP000230750};
RN   [1] {ECO:0000313|EMBL:PIK37127.1, ECO:0000313|Proteomes:UP000230750}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Shaxun {ECO:0000313|EMBL:PIK37127.1};
RC   TISSUE=Muscle {ECO:0000313|EMBL:PIK37127.1};
RX   PubMed=29023486;
RA   Zhang X., Sun L., Yuan J., Sun Y., Gao Y., Zhang L., Li S., Dai H.,
RA   Hamel J.F., Liu C., Yu Y., Liu S., Lin W., Guo K., Jin S., Xu P.,
RA   Storey K.B., Huan P., Zhang T., Zhou Y., Zhang J., Lin C., Li X., Xing L.,
RA   Huo D., Sun M., Wang L., Mercier A., Li F., Yang H., Xiang J.;
RT   "The sea cucumber genome provides insights into morphological evolution and
RT   visceral regeneration.";
RL   PLoS Biol. 15:E2003790-E2003790(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PIK37127.1}.
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DR   EMBL; MRZV01001554; PIK37127.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2G8JN16; -.
DR   STRING; 307972.A0A2G8JN16; -.
DR   Proteomes; UP000230750; Unassembled WGS sequence.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR   PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR   Pfam; PF00632; HECT; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR   PROSITE; PS50237; HECT; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000230750};
KW   Signal {ECO:0000256|SAM:SignalP};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|PROSITE-ProRule:PRU00104}.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           19..534
FT                   /note="HECT-type E3 ubiquitin transferase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5013573864"
FT   DOMAIN          201..534
FT                   /note="HECT"
FT                   /evidence="ECO:0000259|PROSITE:PS50237"
FT   REGION          76..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        501
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   534 AA;  60514 MW;  9D5501F054624030 CRC64;
     MVQLCGFQGL SLWFFASASF GLFRSSVYTG PKVRNRVPES RYPSGIRTED VSVVDDTLQE
     CFICHVEIPE TKLKQHRESC NGKRDDSALP GPSTPVISAP QRLPTSGPVD RYVADYIDLL
     EPDDSSDDEL QHVIEDSLKD IDNNRDDTIL EARMQEKLGQ YHLLREEEKF HVVVRRRKIL
     KCAADAINSQ AGFSFFKDPV ICFSGEEAVD LGGPRREFFT LATQQLSHLG IFEGKTGKLY
     FTHDIVVLEK GLYRLAGQII AWSILHGGPG FPTLHPWLYS MMCDNKPSEE FSTEDVTDSD
     VMGHLQKIEN CSSEALLEDT ITMVGDWAAN NGCPAIYSMS MANKEDILQT LFKQHIYYRC
     KSEIDDFKRG LDSVGKLWSM IHEDPAPFRC LLVMCDHPLT FSSVKKLFHI RWSENCAHRD
     KEEETIYCWE EFLRKAWTGD CSQDIELDDG TTDNIKIELH HILRFCTGAD GIPPTGFHKQ
     IDMEFFSVRD GTKPLPIAST CGLELHLPRG VEDTKFFADM MMEAILCSPG FGKM
//