ID A0A2G8KLL2_STIJA Unreviewed; 1420 AA.
AC A0A2G8KLL2;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=BSL78_14324 {ECO:0000313|EMBL:PIK48828.1};
OS Stichopus japonicus (Sea cucumber).
OC Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Holothuroidea;
OC Aspidochirotacea; Aspidochirotida; Stichopodidae; Apostichopus.
OX NCBI_TaxID=307972 {ECO:0000313|EMBL:PIK48828.1, ECO:0000313|Proteomes:UP000230750};
RN [1] {ECO:0000313|EMBL:PIK48828.1, ECO:0000313|Proteomes:UP000230750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Shaxun {ECO:0000313|EMBL:PIK48828.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:PIK48828.1};
RX PubMed=29023486;
RA Zhang X., Sun L., Yuan J., Sun Y., Gao Y., Zhang L., Li S., Dai H.,
RA Hamel J.F., Liu C., Yu Y., Liu S., Lin W., Guo K., Jin S., Xu P.,
RA Storey K.B., Huan P., Zhang T., Zhou Y., Zhang J., Lin C., Li X., Xing L.,
RA Huo D., Sun M., Wang L., Mercier A., Li F., Yang H., Xiang J.;
RT "The sea cucumber genome provides insights into morphological evolution and
RT visceral regeneration.";
RL PLoS Biol. 15:E2003790-E2003790(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PIK48828.1}.
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DR EMBL; MRZV01000500; PIK48828.1; -; Genomic_DNA.
DR STRING; 307972.A0A2G8KLL2; -.
DR Proteomes; UP000230750; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd11709; SPRY; 1.
DR Gene3D; 2.60.120.920; -; 2.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR045129; RNF123/RSPRY1-like.
DR InterPro; IPR003877; SPRY_dom.
DR PANTHER; PTHR13363:SF5; E3 UBIQUITIN-PROTEIN LIGASE RNF123; 1.
DR PANTHER; PTHR13363; RING FINGER AND SRY DOMAIN-CONTAINING; 1.
DR Pfam; PF00622; SPRY; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000230750};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..30
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1040..1063
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1002..1168
FT /note="SPRY"
FT /evidence="ECO:0000259|Pfam:PF00622"
FT REGION 768..813
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1195..1239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1400..1420
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 790..810
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1209..1236
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1406..1420
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1420 AA; 157281 MW; CF1D76CEC4E262B8 CRC64;
MAPSPGRNNA AIAFAALSAS GGALFAFYIY KRVRELRSQQ FPNRIATAIS NEQLAVLAKS
SNVSLRQSAE QMLLDRILES EAFAEVVQGC YSKDENKLYQ ACSVFCVVLK SVNFSVPLSL
SQIVKLLTSC LQRSLDRESD SEAKSRDSIF SVKIQRMTLA TICDFLSDSI NRKDVLVSSC
PDIIPVCIRL VKEGKNKEVL RYSVLLLFQI AGLGTTQEVR EAGAIPVLAS RIITFHGDQI
MQRITLQTIV VLIGSVDDSK SLCAEIAKPM VLMPVVSSCI SDDVELSFWS LALIQDFVNN
ASFLKDLTEN TALLQTVSES LKNRNDLVVN YCIRFFKILM QGGHSVIEKL LNISDYSILK
GLLHVAKEGQ IENKVLMTEL LTEFVMSEKF VSRIVQNGGL DIVKVLAKPN NEDNLSFFSS
AMLIDMAMIS DEVKGQLAKM YVLPTIYDWI KNAKNEKVAV FATKTLVMLH LIEDLVKISI
NADGKTTQID VGDVHFTDTQ HSSLRIFHWD SLFVNEITIV PIKKKALAST KDQISIQVSR
FDSLSAKNSG EVVFVTLCGT EGSKYVQGLL KYMQMKKDDQ DRCLNLAEDD IAVLIAEVDN
DGCLNLKKMS IASSETEASH SIRYKELFQR RTFSQILPLI MDPLNQKYSQ DGCPLKKKAL
EVLNVLLCLE DYLNMFISSP TAVSNLLMAL QDSSSNGLTV LQSIIGVLVT LATFESGRSH
LISLGAPLVL VNFINHQRDR LVTDLSGSPK GDVFDIDVAP VPPSVAAIIN SLPPRPQSRA
EPRPLRTDDG SVNATDNRSQ ASSSSTNGPL PVIPEDGYIA PEAGETAEAV RLNSLSGLIE
EAVSSAVEVL KNENRKGDNS NTTGPTTSVI IQNAVSCLAK ILSGKMNHSE NDGKYKEFAT
SLRTSGTVYT LWSYLLTSKD DLKALQVPVT NSITILSETI ATDDTAFDAK VRFDRKDVTR
TMFMSTDDLE VLNYNWTFES VRANVGVSQP SNPSPSDHPK GWYYQMELFS DGLIQVGWCS
EQCQYSPQKG NFDENNFLKI SFSVLTIIYL FVYICYTWVH LVFQIQIYRK TVICLWHRHR
DDYHSTPLMA SGKKWHGPVA EQTEENAYGE LWKSGDIVTC LLDCYGNISY HHNGKNLGVA
FAAINMQVCW YPACSVCTDQ WARFNFGDDP VRFPESIPEG FVPISYYKHP PQDAVSNTNQ
PLIDSGDEPA TSNEKEETAE SFDEENTKEV KEGAVKGQPE AGDSAANIVL PLEGARSISA
DYLPSLYFEI KLDSNDARPV SLGFNTLNFT DQVTAVIKDS EIVLPDLSIK QVSVERSVVI
GCGYVASTNH VFFTIDGQAI EVFFEFAGGH VTIPVMPCLS SSRYYVNFGQ RDFQFKIPNT
EEYFKGAIST CVKFLSSCLS PDSEESEPNG NKKELNPLEY
//
