ID A0A2G8L728_STIJA Unreviewed; 2446 AA.
AC A0A2G8L728;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=ubiquitinyl hydrolase 1 {ECO:0000256|ARBA:ARBA00012759};
DE EC=3.4.19.12 {ECO:0000256|ARBA:ARBA00012759};
GN ORFNames=BSL78_06973 {ECO:0000313|EMBL:PIK56077.1};
OS Stichopus japonicus (Sea cucumber).
OC Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Holothuroidea;
OC Aspidochirotacea; Aspidochirotida; Stichopodidae; Apostichopus.
OX NCBI_TaxID=307972 {ECO:0000313|EMBL:PIK56077.1, ECO:0000313|Proteomes:UP000230750};
RN [1] {ECO:0000313|EMBL:PIK56077.1, ECO:0000313|Proteomes:UP000230750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Shaxun {ECO:0000313|EMBL:PIK56077.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:PIK56077.1};
RX PubMed=29023486;
RA Zhang X., Sun L., Yuan J., Sun Y., Gao Y., Zhang L., Li S., Dai H.,
RA Hamel J.F., Liu C., Yu Y., Liu S., Lin W., Guo K., Jin S., Xu P.,
RA Storey K.B., Huan P., Zhang T., Zhou Y., Zhang J., Lin C., Li X., Xing L.,
RA Huo D., Sun M., Wang L., Mercier A., Li F., Yang H., Xiang J.;
RT "The sea cucumber genome provides insights into morphological evolution and
RT visceral regeneration.";
RL PLoS Biol. 15:E2003790-E2003790(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|ARBA:ARBA00000707};
CC -!- SIMILARITY: Belongs to the peptidase C19 family.
CC {ECO:0000256|ARBA:ARBA00009085}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PIK56077.1}.
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DR EMBL; MRZV01000188; PIK56077.1; -; Genomic_DNA.
DR STRING; 307972.A0A2G8L728; -.
DR Proteomes; UP000230750; Unassembled WGS sequence.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR021905; DUF3517.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR PANTHER; PTHR24006; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR PANTHER; PTHR24006:SF827; UBIQUITINYL HYDROLASE 1; 1.
DR Pfam; PF12030; DUF3517; 2.
DR Pfam; PF00443; UCH; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS00972; USP_1; 1.
DR PROSITE; PS00973; USP_2; 1.
DR PROSITE; PS50235; USP_3; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000230750}.
FT DOMAIN 1524..1913
FT /note="USP"
FT /evidence="ECO:0000259|PROSITE:PS50235"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 923..951
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1562..1601
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2366..2446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 925..946
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1579..1601
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2379..2408
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2418..2446
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2446 AA; 279310 MW; 1AE5A8526E12B1E2 CRC64;
MTVETKTGPG IQDGEAGEEN DSNEVEFPLD ALSRLDTMIN KPRWIIPVLP KGELEVLLEA
SIELCKKGLD IHCEPCQRFF QDGLMTSFTK ILTDEAVSGW KFEIQRCILK NSEKLIELAV
IKLSQDWFPL LDLLAMALNP HCKFHVFNAT RQSETAPEEI GTERFAKPPD ARTPKGWLVD
LINKFGSLEG FEILQQRFLS KDQPLNVSVI HALIRPWGMC YEMLTQNTVQ TFFMPIVDRI
VEYLDSLSDE ELKKESKNES RNDALSLIIK SLKNLVSRVP KQEEKIKSLE MFRLKMILRL
LKISSFNGKM NALNEVNKVI SNVSYYTHKH SHVEDEEWLT AERMAEWIQE NKVLSIVLRD
NLHQPQYVEK LEKILRFVIK EKALTKEDLD NIWAAQVGKH EAIVKNIHDL LAKLAWDFSP
EQLDHLFDCF QESWTSASTK QREKLLELIR RLAEDDKDGL MAHKVLSLLW TLAHQDDVPT
DIMDQALHAH IKILDYSCSQ DRDAQKIQWI DKFVDELKND KWVLPALKQI KEICSLFGEA
PQNYTHAQHS PHLQYRHDVI NKLQGKHSLV TLVAGNLSNY MNKARKISKE NPSVDPQDLV
PDSRYTHAQQ VQERLNFLRF LLKDGQLWLC APQAKQIWTC LAENSVYVSD REACFKWFSK
LMSDEPDLDP EINKDFFELN ILLLDPSLLT ENGIKCFDRF FKAVNSKEGK LIPKRRAYLM
DDLELIGLDY LWRVVLCAPD EIAAKAIELL KETYTNLGPR LQANQVDIHI DFIQSCMDRL
KAMYDTLRSL EEGKDGPDKL QQEATRMTRV LSVLREYVHE YDLDFMEERT ILPMARAHRG
KHVTLQIRFS NQNRQGEDLD IWSHTNESLA SVRRQILLRV KASPATTRLD LYINGEFLDP
TDDRKLIGSM ALRDRTLITA KLCPIGSNMP SSPDSSSDSS GGSPQHPCDG PNIEAEKCLP
GVLMSTKLKY VEFLFNLADI GCVLGIPSLR GEARALLKII PGARTMSQGE KRPVTTAVFI
LVYGNDDNLV PSLNQSFLTI DVLVCSSYTV RGKEETKLIS LFPCLFDLTD ARIVEQLQEV
CASAAENNTQ SLESVFFGTS PSQVLYFLEV VYAMLMPAKA PTGPEAFKFQ FNFLKSGGID
CVINMLTKNN FLQNGDLDTR RWALHLVLKT GKLMFTTAGY AKLAAAQEAY QSDPNTGPSI
SQAAHTQAVI LQQALQHIPN PTTECMLRNV AIKLGRSVSE DTEGSEADLD DANLCREALE
VLSICLALCP TALDALNQEK HWKMCIVDLL LLAPMRHVRL AAMEQFLVMA TKCTTGTTAL
HVLITLSFSV LQNKVKENFE SCGEYFGLLC RLLNYAYQSN NPIPNAEILL NNEIDWLKKV
RDEVLRTGES FSQESLLDGH LGITKELLSF MSSEKKFQIG AKKDGCQLIK ELMEDFLFPA
SKVVVQTRKN KAETPLDPVV PVCASSASVN AAFELLVALC VNCVANMKLV VDTLREMYYS
GHEAPLVEWE YLPPIGPRPH KGFVGLKNAG ATCYMNSVLQ QLYMVPDIKK IVLAAESPAI
EHEDDFDGDE KQDNETNIDF GDSEVSGSGY NEEKPTTLSK QEQAKEYNLG VLKNIQAIFA
HLAESKLQYY VPRGFWKHFK LMGEPVNLRE QHDALEFFNS VVDSLDEALK AVGASAVFLK
CLGGSYADQK ICKGCPHRYS RETMFTSMNV DIRNNTNLFD SLEQFVKGDL LEGDNAYLCE
KCNKKVDTVK RMCVKKCPKI LAIQLKRFDY DWERECAMKF NDYFEFPREF DLEPYTVEGL
AKLEADHNTI PFPGEEIQAD ERETSDLEED LEDPNRCTKY RLIGVVVHSG QASGGHYYSY
ILQRQSVNDV EAKWYKFDDG EVMDCRMDDD EDVVQEAEEM VECLPVVLRE NGHRQEGDTQ
DMENLTITPK SSSLKMPPAI QRSVRKKNIQ FMHSRMQYSQ EYFLFMRKLV GCNRSLFNPP
SQQSADVQLS QDVEELAMQS IQLASWFLFS VGFHTKKTLR GPAAEWYDTL YGLLCSSKTV
RRWFSTNVLF AHRNRFSEYL LECPSSEVRN SFAKVIVILA HFSQNDGTFQ MNGHVASLSE
HLLMEVLHLL KKEVSEHGRH LTQYFQLFQM YANLGAAENA EPSPNPFRDS SCTQPLMAVQ
PRVEELLYSR GGYVKKLIED CNSSEETTKL LRFCCWEHPQ FSLLVLGELL WQIAFAYTYE
LRPYLDLLRQ ILMMEDSWQQ HRIINALRGI PEDRDGLFDT IQRSKNHHQK RAYQCIKCMV
VLFSNCQIAQ GLLNNHTDLK RKWHQAVEWL SDELERRSYS SNTQYTYNNW SPPAQSNETS
NGYFLERSNS ARMTLAKACE LYPDEEQEEV EVMDEQEGSD SAMTYNSQPE ITAQNSQQQP
QPLNNQQPEH RQHRNREPPN TINNTFTFPR SRQQNLQHRP PGTRGS
//
