ID   A0A2H3DHY8_ARMGA        Unreviewed;       568 AA.
AC   A0A2H3DHY8;
DT   31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT   31-JAN-2018, sequence version 1.
DT   24-JAN-2024, entry version 20.
DE   RecName: Full=amidase {ECO:0000256|ARBA:ARBA00012922};
DE            EC=3.5.1.4 {ECO:0000256|ARBA:ARBA00012922};
GN   ORFNames=ARMGADRAFT_68817 {ECO:0000313|EMBL:PBK94831.1};
OS   Armillaria gallica (Bulbous honey fungus) (Armillaria bulbosa).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Marasmiineae; Physalacriaceae; Armillaria.
OX   NCBI_TaxID=47427 {ECO:0000313|EMBL:PBK94831.1, ECO:0000313|Proteomes:UP000217790};
RN   [1] {ECO:0000313|Proteomes:UP000217790}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ar21-2 {ECO:0000313|Proteomes:UP000217790};
RX   PubMed=29085064; DOI=10.1038/s41559-017-0347-8;
RA   Sipos G., Prasanna A.N., Walter M.C., O'Connor E., Balint B., Krizsan K.,
RA   Kiss B., Hess J., Varga T., Slot J., Riley R., Boka B., Rigling D.,
RA   Barry K., Lee J., Mihaltcheva S., LaButti K., Lipzen A., Waldron R.,
RA   Moloney N.M., Sperisen C., Kredics L., Vagvoelgyi C., Patrignani A.,
RA   Fitzpatrick D., Nagy I., Doyle S., Anderson J.B., Grigoriev I.V.,
RA   Gueldener U., Muensterkoetter M., Nagy L.G.;
RT   "Genome expansion and lineage-specific genetic innovations in the forest
RT   pathogenic fungi Armillaria.";
RL   Nat. Ecol. Evol. 1:1931-1941(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00001311};
CC   -!- SIMILARITY: Belongs to the amidase family.
CC       {ECO:0000256|ARBA:ARBA00009199}.
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DR   EMBL; KZ293653; PBK94831.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2H3DHY8; -.
DR   STRING; 47427.A0A2H3DHY8; -.
DR   InParanoid; A0A2H3DHY8; -.
DR   OMA; EYHAPIS; -.
DR   OrthoDB; 731186at2759; -.
DR   Proteomes; UP000217790; Unassembled WGS sequence.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; Amidase signature (AS) domain; 1.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR46072:SF10; ACETAMIDASE; 1.
DR   PANTHER; PTHR46072; AMIDASE-RELATED-RELATED; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   PIRSF; PIRSF001221; Amidase_fungi; 2.
DR   SUPFAM; SSF75304; Amidase signature (AS) enzymes; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000217790}.
FT   DOMAIN          61..545
FT                   /note="Amidase"
FT                   /evidence="ECO:0000259|Pfam:PF01425"
FT   ACT_SITE        108
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   ACT_SITE        183
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   ACT_SITE        207
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   BINDING         157
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
FT   BINDING         183
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
FT   BINDING         204..207
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
SQ   SEQUENCE   568 AA;  62828 MW;  EE9B7B56D30768FB CRC64;
     MASFLSHWKA CHVKQEERKK RIEDLDPAYH EPLSNKDAEI LGRPIADLVA SVQSGACDSD
     ILVAYSKQAL KAHAKTNCLS EVMIASAETW AKECNKKGPL AGVPVSLKDS VGVKGWDSTI
     GYSAWVGQPI QEDGPLVRLL RDAGAVPFVK TTIPITLLSF ESSSDLFGRT TNPHNPAYSP
     GGSSGGEAAL LAFGGSRIGL GTDVAGSVRT PSHYSGVYTI RSSTTRFPKL GGVTSIPGQE
     GVAAVCSPMA RTLADLETFW KAVVSQKPWD YDHTCLNLPW RDMDVSKKSL KWGVMWDDGV
     VQPSPACKRA LYSVVETLQN HGHEVFTMWV WPPFLPSGAY TMASRPAPSP YEGLQIAFQL
     LLADGGKTVM KPFQMGETND PGVSQARIMF SLPRWARRLY VWYLRYLRGD EIYAGLVEGC
     YEKSITEYWP LVAQREAYRR RWFDMWNEQN LDFLLTVPNA LPAVPHGGMR EGFKSCGYTF
     LFNLLDYTAG VLPVTHVNEK LDVLKGFKPR NAVEAGAYKD YDSVKMDGLP VGVQIVGKRL
     EEEKVMEGMK LIERLLKEDG KAYELLNV
//