ID A0A2H4ZU23_9VIRU Unreviewed; 1539 AA.
AC A0A2H4ZU23;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=RNA replication protein {ECO:0000256|ARBA:ARBA00018318};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
OS Babaco mosaic virus.
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Alphaflexiviridae; Potexvirus.
OX NCBI_TaxID=2060511 {ECO:0000313|EMBL:AUG45968.1};
RN [1] {ECO:0000313|EMBL:AUG45968.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tandapi {ECO:0000313|EMBL:AUG45968.1};
RA Alvarez-Quinto R.A., Cornejo J.F., Quito-Avila D.F.;
RT "Characterization of a not so new potexvirus isolated from babaco
RT (Vasconcellea x heilbornii) in Ecuador.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA replication. The central part of this protein possibly
CC functions as an ATP-binding helicase. {ECO:0000256|ARBA:ARBA00025585}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MF978248; AUG45968.1; -; Genomic_RNA.
DR Proteomes; UP000235595; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23246; Alphaflexiviridae_RdRp; 1.
DR Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001788; RNA-dep_RNA_pol_alsuvir.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022806};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000235595};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}.
FT DOMAIN 59..227
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000259|PROSITE:PS51743"
FT DOMAIN 781..1074
FT /note="(+)RNA virus helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51657"
FT DOMAIN 1316..1423
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
FT REGION 434..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 469..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1069..1088
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..510
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1539 AA; 173466 MW; F21FB6B4DEB6448D CRC64;
MANFRAVMDQ LNDPSLRAVV QEEAYREIRK TIEVTKTYNP YAQSSLAADS LEKLGIETNP
LAVRAHTHAA AKSIELDMYK IASHYLPKEN PVTFLFMKRA KLQYFRRGPQ QNDVFLNAHV
EPKDVVRYDP DDLFDADTTP KIQTSVAFMG DALHYFPLSV INKIFTASPK LKTLYATLVL
PAEAAHRMHS LHPSIYELQF HEENFLYKPG GHAGAAYCHT YKQLEWLQVG RFRWETASGE
ERNVTSQILE TKGANHLMVF QRGLYNTPTL RCFGVETKYV TMPPIFLPAK FNARLPIRKT
VAQQLFLYVK SVKSVTERDI WAKIRQLIKT ADLQEYSSKE LTLLVNYFFL ISKLKSETCF
DSILSGGMIK RVFKPLISWI NEKKGFIFGK DQFIQLMEAL EWVEIDLTYK TKTFDEPNDK
SSFNPMGYLP LKDEEGLPGL QESSSDQPQA TEEDQVAYDK YLKALSRLQA SDESEERESG
PPPSNSESEA SSSGGNQAEE TNSCPPNSTE KSRGLDAILC PCGLSIPLGK AKFPEIPVLE
HPERLKGRDA FFFSKDNKPY SYTGGSHHSR GWPSWLDSIL AAVEVQETMP NFNQCLVQKF
SARSSIPFHR DDEKCYPKGH QVLTVNLKGT CTFKICCDRG VGTANLEEGT YHLSPPGFQE
NHRHAVEGCS AGRVSLTFRS TAIQEQHSEG LVAAPLDSLP WKAWIPKLQE LGFQGRQLQY
DCNGALISPI EQIQSMEKTR PKGVPASLLT CLEKIARAPT HFSPSPIRAK AYSSDVKNAR
IGAMLKQQGK DWGHRFDALV EAGKRSLHIS VIHGAGGSGK SRAIQNYIKE HTKEPVQVIL
PTNELRIDWM RKVPTLKETM CKTFEKALLA PPTPVVIFDD YGKLPAGYIE AFCFFFSTVE
LVILTGDSKQ STHHESNENA MTNLIEPFTS EASKLCRYYV NATHRNKKDL ANKLGVYSEV
SGFTEITHSS SPIPGLHILV PSLYKKQAFS EMGHKASTYA GCQGITAPKV QILLSEETTL
CSKEVLYTAL SRAVHSIHFI NTSPNNQSFW KKLESTPYLK AFLSTLREET AQESKPAQGD
PTAVDPPKTH IPVDSALPIY ENILDSMPEK HEREIFSKSH GHSNCVQTED SFVQMFSHRQ
AKDETLLWAT IEARLVVSNP KSNWQEYLEK KPIGDVLFES YKKAMKLPAE RIAFEPQLWE
SCIHEVQGTY LKKSEDMIKN GQARQSPDYD PNLISLFLKS QWVKKMEKLG ALKIKPGQTI
ASFQQATVML FGTMARYMRR MREVFMPKNI LINCETTPES MSSWAAGEGG CWSFKGPSLA
NDFTAFDQSQ DGAMLQFEIL KAKHHSIPED ILDAYISIKT NSKIFLGTLA IMRLTGEGPT
FDANTECNIA FTHTKFQIPD GTAQIYAGDD SAIDALPVVR KSFKQIENKL TLKSKPLVAL
QQKGDWAEFC GYRITPAGFI KDPKKLHASL MLEVKKKNIK NVRRSYELDL ALAYQHRDRL
HELLSEEELR FHYETVRTLV KSGGGEVLKT FLPKDESLY
//
