ID A0A2H5B288_9ACTN Unreviewed; 473 AA.
AC A0A2H5B288;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex {ECO:0000256|RuleBase:RU003423};
DE EC=2.3.1.- {ECO:0000256|RuleBase:RU003423};
GN ORFNames=CFP65_3707 {ECO:0000313|EMBL:AUG78493.1};
OS Kitasatospora sp. MMS16-BH015.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Kitasatospora.
OX NCBI_TaxID=2018025 {ECO:0000313|EMBL:AUG78493.1, ECO:0000313|Proteomes:UP000239175};
RN [1] {ECO:0000313|EMBL:AUG78493.1, ECO:0000313|Proteomes:UP000239175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MMS16-BH015 {ECO:0000313|EMBL:AUG78493.1,
RC ECO:0000313|Proteomes:UP000239175};
RA Kim S.B., Yun B.-R.;
RT "Genome sequence of Kitasatospora sp. MMS16-BH015.";
RL Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088;
CC Evidence={ECO:0000256|ARBA:ARBA00001938,
CC ECO:0000256|RuleBase:RU003423};
CC -!- SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00007317, ECO:0000256|RuleBase:RU003423}.
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DR EMBL; CP025394; AUG78493.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2H5B288; -.
DR KEGG; kit:CFP65_3707; -.
DR OrthoDB; 9805770at2; -.
DR Proteomes; UP000239175; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd06849; lipoyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 4.10.320.10; E3-binding domain; 1.
DR InterPro; IPR003016; 2-oxoA_DH_lipoyl-BS.
DR InterPro; IPR001078; 2-oxoacid_DH_actylTfrase.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR036625; E3-bd_dom_sf.
DR InterPro; IPR004167; PSBD.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR43178; DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX; 1.
DR PANTHER; PTHR43178:SF5; LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL; 1.
DR Pfam; PF00198; 2-oxoacid_dh; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF02817; E3_binding; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
DR SUPFAM; SSF47005; Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR PROSITE; PS00189; LIPOYL; 1.
DR PROSITE; PS51826; PSBD; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003423};
KW Lipoyl {ECO:0000256|ARBA:ARBA00022823, ECO:0000256|RuleBase:RU003423};
KW Reference proteome {ECO:0000313|Proteomes:UP000239175};
KW Transferase {ECO:0000256|RuleBase:RU003423}.
FT DOMAIN 8..83
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 176..213
FT /note="Peripheral subunit-binding (PSBD)"
FT /evidence="ECO:0000259|PROSITE:PS51826"
SQ SEQUENCE 473 AA; 48494 MW; 50A43E65E0A6C772 CRC64;
MTTEVRSLRE FKMPDVGEGL TEAEILTWFV KVGDTVTDGQ IVCEVETAKA AVELPIPFNG
TVEQLFFPAG TTVDVGTSII SVAVEGAAPA AAPAPAPVAA PAAAPAAAPA AEAEPEPERR
AVLVGYGPRT GGVQRRARKT TVVATSAPAA AAPVAAPAVP APAPVAEPVQ TGERPLAKPP
VRKLAKDLGI DLRTVTPTGP DGVITREDVH AAAAPAPAAV AEAVAPVVEA APVLPVAVGA
GDVRVPVKGV RKATAQAMVA SAFTAPHVTE FVQVDVTRTM KLVRELKESG ELGAGVRVSP
LLIVAKALLT AVRRYPEINA SWDEAAQEIV IKGQVNLGFA AATPRGLIVP NIKDAGSKTL
PQLAASLGDL IDTARQGKTP PADMSGGSIT ITNVGVFGVD TGTPILNPGE AAILAFGAVR
DLPWVHKGKV VPRQVTTLAL SFDHRLVDGE LGSKVLADTA AILERPKRLI TWG
//
