ID   A0A2H5F2V7_9RHOB        Unreviewed;       562 AA.
AC   A0A2H5F2V7;
DT   28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT   28-FEB-2018, sequence version 1.
DT   24-JAN-2024, entry version 23.
DE   RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU003887};
DE            EC=5.4.99.- {ECO:0000256|RuleBase:RU003887};
GN   ORFNames=CX676_18235 {ECO:0000313|EMBL:AUH65857.1};
OS   Paracoccus zhejiangensis.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC   Paracoccaceae; Paracoccus.
OX   NCBI_TaxID=1077935 {ECO:0000313|EMBL:AUH65857.1, ECO:0000313|Proteomes:UP000234530};
RN   [1] {ECO:0000313|EMBL:AUH65857.1, ECO:0000313|Proteomes:UP000234530}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J6 {ECO:0000313|EMBL:AUH65857.1,
RC   ECO:0000313|Proteomes:UP000234530};
RX   PubMed=23653121; DOI=10.1007/s10482-013-9932-2;
RA   Wu Z.G., Zhang D.F., Liu Y.L., Wang F., Jiang X., Li C., Li S.P., Hong Q.,
RA   Li W.J.;
RT   "Paracoccus zhejiangensis sp. nov., isolated from activated sludge in
RT   wastewater-treatment system.";
RL   Antonie Van Leeuwenhoek 104:123-128(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000256|ARBA:ARBA00000073};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000256|ARBA:ARBA00008348, ECO:0000256|RuleBase:RU003887}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP025430; AUH65857.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2H5F2V7; -.
DR   KEGG; pzh:CX676_18235; -.
DR   OrthoDB; 9807213at2; -.
DR   Proteomes; UP000234530; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.30.70.1560; Alpha-L RNA-binding motif; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR   PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR   PANTHER; PTHR47683:SF3; RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE B; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003887};
KW   Reference proteome {ECO:0000313|Proteomes:UP000234530};
KW   RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT   DOMAIN          18..76
FT                   /note="RNA-binding S4"
FT                   /evidence="ECO:0000259|SMART:SM00363"
FT   REGION          251..562
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        253..506
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   562 AA;  63222 MW;  4FBF66E34A373EA6 CRC64;
     MTDDQTPDSN TDKPADVDRI AKVIARAGVA SRRDAERIIL EGRVTVNGEK ITSPALDVRP
     TDRITVDGKP LDAPQETRLW MYYKPVGLVT SEADEKGRQT IFDALPRDLP RVMTVGRLDL
     NSEGLLLLTN DGELKRRLEL PATGWLRRYR VRVNGTPTDL TFDPLRRGVT IDGEDFQPME
     VRLDSQQGAN AWLTVGIREG RNREIRRAMT HIGLQVNRLI RIGYGPFKLT GMEENEVREV
     KRKVLRDQLG GLLTGETEDE KPRDFRARGA ESGPRRSAAD GERKPRFGKP DGERKPFGRN
     DDRGERRDGG KPFARREDGD RKPWAKREDG DRKPFARRED GDRKPWAKRE DGDRKPFARR
     EDGDRKPFAR REDGDRKPWT KREEGDRKPF ARREDGDRKP FARREDGDRK PWAKREDGDR
     KPYAKREDGD RKPWAKREDG DRKPFARRED GDKKPWVKRD RSDEGFKPRG EGGFKPRGEG
     GFKPRGEGGF KPRGEGDFKP RGEGGFKPRG EGFNSGGPRK GGFSGGPAGG KGRPAAGKGP
     RPGGGKPGGG RPGGGKPRAP RG
//