ID A0A2H5NDF5_CITUN Unreviewed; 1182 AA.
AC A0A2H5NDF5;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=CUMW_035030 {ECO:0000313|EMBL:GAY38218.1};
OS Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=55188 {ECO:0000313|EMBL:GAY38218.1};
RN [1] {ECO:0000313|EMBL:GAY38218.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=29259619; DOI=10.3389/fgene.2017.00180;
RA Shimizu T., Tanizawa Y., Mochizuki T., Nagasaki H., Yoshioka T., Toyoda A.,
RA Fujiyama A., Kaminuma E., Nakamura Y.;
RT "Draft sequencing of the heterozygous diploid genome of Satsuma (Citrus
RT unshiu Marc.) using a hybrid assembly approach.";
RL Front. Genet. 8:180-180(2017).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAY38218.1}.
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DR EMBL; BDQV01000006; GAY38218.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2H5NDF5; -.
DR STRING; 55188.A0A2H5NDF5; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02736; RNAP_III_Rpc1_C; 1.
DR CDD; cd02583; RNAP_III_RPC1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 2.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR035698; RNAP_III_Rpc1_C.
DR InterPro; IPR035697; RNAP_III_RPC1_N.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF32; DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 2.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279}; Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 239..516
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT COILED 266..293
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1182 AA; 131753 MW; 4D584D7411341578 CRC64;
MTQQLQQKSQ SIAFTKQPYI EDVGPRKIES IQFSMMSDSE IMKAAEVQVY KGQYYSSNRV
PIEGGLLDPR MGPANKSCTC ATCHGGFSNC PGHYGYLTLA LPVYNVGYLS TILDILKCIC
KYCSRLLLEE KLCKDYLKKM RNPKMEALRK TDLMKSIVKK CSTMASMVKK AVAVLGIIHD
RSKVTESLQE FASAITHTKE SKAAVNVATY MLNPVNVLFL FKRMTDADCE VLYLSERAEK
LIITNIAVPP IAIRPSVIMD GSQSNENDIT ERLKRIIQTN ASLQQELVEA NSAFKSLAGW
ETLQVEVAQY INSDVRGVPF SMQVARPLSG FVQRLKGKQG RFRGNLSGKR VEYTGRTVIS
PDPNLKITEV AIPIRMARIL TYPERVSDHN IEKVLYGKFR NQLAVELKSG CIVDRHLEDG
DVVLFNRQPS LHRMSIMCHR ARIMPWRTLR FNESVCNPYN ADFDGDEMNM HVPQTEEART
EALLLMGVQN NLCTPKNGEI LVASTQDFLT SSFLITRKDT FYDRAAFSLM CCYMGDGMDR
VDLPTPAILK LISGQLGKAT LGNGNKDGLY SVLLRDYGAH ATSGCMNRLA KLSARWIGNH
GFSIGIDDVQ PKKELSDKKG KLISENYEVC NVKIKEYNEG KLQLKPGCDA AQTLEAVITD
ILNRIREDAG KACMGSLPWR NSPLIMSQCG SKGSPINISQ MVACVGQQSV GGRRAPNGFI
DRSLPHFPRK AKEPAAKGQQ DKLDKKIKFV EDIGWDDKSQ ILEEVTHKTS GITEKQLEVF
IQTCFSRYRV KRVEAGTAIG AIGAQSIGEP GTQMTLKTFH FAGVASMNIT QGVPRIKEII
NGAKRISTPI ITAELECNDN ENAARVVKGR IEKTLLGQVA KSIKIVMTSR LASIVIALDM
ETIQDAHLCI NADIVKESIV QTPKIKLKQQ HIKVLDFRKL EIFPPVDKSK IHFELYSLKN
VLPMVIVKGI KTVERVVIAE KEKEKKKVKE NGEKKKYRLL VEGTGLQAVM GAEGIDGCKT
KSNHIFEVQQ TLGIEAARIC IIDEINETMK AHGMSIDKRH MMLLADLMTF RGEVLGITRF
GIQKMDKSVL MLASFEKTAD HLFNASVNGR DDRIEGVSEC IIMGIPMPLG TGILKIRQRC
CASNAEIWGP RSNYVMTSSN FHNYEAEVAE DQSHQTALRL LC
//
