ID A0A2H5NE66_CITUN Unreviewed; 5498 AA.
AC A0A2H5NE66;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Midasin {ECO:0000256|PIRNR:PIRNR010340};
GN ORFNames=CUMW_037120 {ECO:0000313|EMBL:GAY38486.1};
OS Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=55188 {ECO:0000313|EMBL:GAY38486.1};
RN [1] {ECO:0000313|EMBL:GAY38486.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=29259619; DOI=10.3389/fgene.2017.00180;
RA Shimizu T., Tanizawa Y., Mochizuki T., Nagasaki H., Yoshioka T., Toyoda A.,
RA Fujiyama A., Kaminuma E., Nakamura Y.;
RT "Draft sequencing of the heterozygous diploid genome of Satsuma (Citrus
RT unshiu Marc.) using a hybrid assembly approach.";
RL Front. Genet. 8:180-180(2017).
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAY38486.1}.
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DR EMBL; BDQV01000007; GAY38486.1; -; Genomic_DNA.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 3.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 7.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 8.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR PIRSF; PIRSF010340; Midasin; 2.
DR SMART; SM00382; AAA; 5.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340}.
FT DOMAIN 329..475
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 688..930
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1078..1223
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1369..1609
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1792..1944
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 4625..4650
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4664..4945
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4963..5014
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5063..5087
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5123..5154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 3936..3985
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 4664..4691
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4701..4718
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4733..4778
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4808..4833
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4834..4863
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4864..4878
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4963..4991
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5000..5014
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5067..5081
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5135..5149
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5498 AA; 624695 MW; 2DD70AEB12476062 CRC64;
MAIDGSFSLE SALERFLARC PKVGGLRHFE SLVRKGNKVT EEEVVNSVAE LSLHPHYTIP
LLGCFRPIAQ KIVDRAVDLL HHVSDDLRSN SDEQGEDSGY LGDAEVVAVI QFHVNSRSGL
RLHELACLAF CRVVSHYLNA ARISYRLLII NTEILSKLWD WSCFLDFVKK FGSLNLYSDA
EFKNDIADIR WCGLQILSVI LRTSDRAIVD FGVQAEESIS CLLRWEEFCC DTSLEKAASY
IESSDKLGST IDDLSFNQEN CLRCFGLYSQ VYSQVNEIEP STRCRKLAIR DNKSRLISEF
RDDKSDSNPF VLTSTVKRSY EVVLLAVSQK WPVLLYGPTG SGKSALVNNL AKESGNQVLS
IHMDDQIDGK TLIGSYVCME QPGEFKWQPG SLTQAILNGY WVVFEDIDKA PSDMQSVLLP
LLEGARSFAT GYGEEIRIPE SFRLFSTIST SNIILQDKFI RHLVLSISPL SHQSLQSVSK
GLIMVSRDLE RATTNSNPLP FCQRSLAIVM FHIPFCLVCV GGNSLGNLWR RVMIMPPSND
DLQNIVKSWY PNLESLTERL IETFERIQSR NSVSYGSLSR YSLRDLLKWC KRIACFDFSS
RMNGLSADEC HDIYQEAVDI FASFSASAEN RLIMMKELAK MWAVPVSTIE SLYPSHEPVI
QLHEETRKFV SIRSSLHVLE KIACSVNFNE PVLLVGETGT GKTTLVQNLA GMLGQRLTVL
NLSQQSDVAD LLGGFKPMDA RFICIPLYKE FEYLFSKTFS MMENNKIFFC LQKLLSDRDW
KKLLKGLCKY VDEYKKGKKR KKYLDEEWEI FSLKLEAACR QIASPGMIFS FVEGAFVNAL
RKGEWILLDE INLAPPETLQ RIIGVLEGDN GSLCLAERGD VSHICRHPEF RIFACMNPAT
DAGKRDLPYT LRSRFTEFFV DDVLDDKDLE LFIERFLGES RSNRERVQKI RCFYKAAKKE
SEEKLQDGAN QKPQYSLRSL YRALEFTRKA EGKFGFERAL YGGFCMFFLT MLDGPSAKIM
KQMIGHWLVK SVPASVPFDN YLNIERGILM SDDFLKNYVL TKSVREHLSN LARAIFIKRY
PVLLQGPTSS GKTSLVKYLA ALTGREFVRI NNHEHTDIQE YLGSYITDAC GRLVFHEGVL
VKAVRNGHWI VLDELNLAPS DVLEALNRLL DDNRELFVPE LCETIKAHPD FMLFATQNPP
TSYGGRKMLS RAFRNRFVEI HVDEIPENEL STILEKRCEI PETYAKKMVE VLKELQLHRQ
SSKVFAGKHG FITPRDLFRW ANRFKAFGKS YEDLARDGYY LLAERLRDES EKCEVQEVLE
KALRVRLVKD DLYPQEQAGS DLVSERLGNV VWTKSMWRLY FLVKRCYELR EPVLLVGETG
GGKTSVCQML SLVLGSKLRI LNCHQYTETS DFIGGFYPVR DRSRLMSEFK HLIEQRLKSE
LKHLVEQWNP LNGDSEISSD IRQASSTLGK LAEIVKCCRD GQICGAAPQE LDSLEQLMLD
VIQLHQRWQT IFMWHDGPLV QAMKDGSLFL VDEISLADDS VLERLNSVLE AERKLSLAEK
GGLIMENVTA HEDFFVLATM NPGGDYGKKE LSPALRNRFT EIWVPSVSDL DELGNISLQR
IANPGLSALV GPMLNFWESF SHLQTGRTLT VRDLLSWVAF INVTERSLGP TYALLHGIFL
ILLDGLSLGT GISERDAGVL RKICLSFLLE QFKEEDNSVL YAKISSMENY GWGDIGTNAD
SPCSDAMQCD NIFGIDPFYI EKGDEKTEVG SFDFSAPTAR RNALRVLRAM QLSKPVLLEG
SPGVGKTSLI VALGKYSGHK VVRINLSEQT DIMDLLGSDL PAESDEGMKF AWSDGILLQA
IKEGCWVLLD EINLAPQSVL EGLNAILDHR GEVFIPELGL TFRCPSSFRV FACQNPSYQG
GGRKGLPRSF LNRFTKVYVD ELVEDDYLSI CSSRFQSIPR SLLLKLVLFN KRLHKEIVVY
RKFAQDGSPW EFNLRDVMRS CQILKGAPEK TKCDYFLNIL YLQRMRTADD RQEVIRIYEE
IFGTKPFINP YPRVQLNPQY LVVGNTAIKR NYTQCSKVLS SQLKILPGIR HSLEAAAHCI
KEQWLCILVG PQSSGKTSLI RLLAQLTGNI LNELNLSSAT DISELLGCFE QYNVFRSFRM
VVAQVESYIN KYSSLQLESS GEAIIVGKKE LISRWLSFSS SVDFTLLSSS HSAYKENWKR
ISNSLRLLVE IIEKLRLDLG NNRCDELNRM EKTILKLQDN LKLLQSAKFE WVTGSLIKAV
ENGEWIVLEN ANLCNPTVLD RINSLMEPSG TITINERGTV DGKPVVLGPH PNFRMFLTVN
PSYGEISRAM RNRGVEIYMM PPYWLFDKGS GFTFEDSELK DANRFLVLSG IPGGKLVESM
SKAHVYAKCE GLRFNKSITH LELARWAQLF QRLLINGNQP KWSLQISWEH TYLSSLGVGE
GENIINKASN TYLSMIESSE SASLFDSSLC MPGGWPMPLK LRDFIWYSKE TSVRQNCMYL
EFLGSQFASG ELQNCWNGSP VDHALTASNC SMTYLMNIEM LQSIMFPKIS NRVISSSSGK
REYNSNLTKK MLLFAANWTI EQATESDFQL HLLWLSWFSS QLQPYHQFFN SFLTSLKKEL
EDQIWKDLFR LHRKLTSLSS VNLDLHPIPV LSMELVDLTA SDDMSKVQLC NAINCVDLLR
LSYQQWNAEN EHDYSDESLY FQPFLDSVKN LEKKVLKMLV KSPSFDKLLQ LCTVLLEDHI
LFWNGITSSK FDFLLISWRS LMKDARKLHE FCPREVQNVL MEGESLAEVS SWHFQSERSL
LWVHGGHPFL PQSAKLYHQQ HQLLELCESL WQKQASDCLV DVVASSNPEF RYLALQGLCM
SSHIACKSNE DDLCIAQQLE DVYQMLVRRF EYEKRKLEAN LERDQLFDSD LASCCVFHSE
VLCKTPGYDS WFDILPINDS ASWFLDMELL QELSAISIVD HTELQLALSS VSHLLESALK
YSLTASRRPP QTFVPHQKLL WMLDAWMSVD AAHVKVASFV LEMWFHWHSF LWSYHPAPLM
SLSSKGNHDI PLPQLLIQPV KTAVVFQILQ SRDAIKDYSV YCLKLKVASR NFWESPAPKS
FPSSLLAVAR SIFNQIICAH KKSFDAGKFA EIKSMLCAFG KSVVTQDSIC HLNSLIASSS
HQRLKSLVHL FIEPLLRELY LHCSPIGLHA NLGFAWLFIG GLRFHLLLSC DDLDPAMKYS
WKCSQLEEKI SLLELEIKVR QECNYLCGWS PSREADKKRA AALQMLEVEQ KRLQRKIVFR
PDPLKFKALK KECDEFLELF NSSTNLVKNI AIMDLQNAIQ QMCNWQETAS SFICRLSEEY
PEFIDLAQPV QVAVYEMKLG LSIVLSSTLQ KVFQERIDDD MDQVMESLYL FMRFPRTYGF
GSDSVNFKSR LPECYFHGLD FSSNLCQMCI SFLEKMVTFQ SDINAAKQGS VLQLRASVYQ
NALIRVAHSV ANSRLMDKAS FMLLDKIFSE FASIWTNMKD EVKTKENHNA QQYKFRPRAF
KVDRVFEVEK SSLRKFFAND AFSEWQELLL EEEFAEKLEA GDEHESLEEE WNLMQESILD
NMVYIHNQLF GSTNLILSSG TFQISDAERL LSFSDSYTLG LEMIKGLECL FTSTLDAKLA
PEHLLRICLE HEKIVSSNHS ARKYNFYKDS NAHVMAKMVK LLTTLQQRVL MCLSEWEDHP
GLQKILNMIE MLLAIPLTTP LAKPLSGLQI LLRHVQMLQE NGMKFPLSDL LEPIIVLVSS
WQRMEFESWP VLLDEVQDQY EKNAGKLWFP LFSVLAHTHS DKVAGYDQST LHRQAIRNYL
VAEFKFEVIN RECCESIKFG IFLSNTNGYG FCSLEEFIQT SSIGEFRKRL LLIFAFLGQF
VIGRSLEAYS SLWQEENLKM LYNIFGFYVQ FLPLILEHIG NNRKNIEKEV KELLKLCRWE
HFMPIENLKR IRQKLRKLVQ KYTVSGILLL PSNGYDKELL QQPAMLILNQ ETAQKGLNVL
SIQGQKAPTE ISDMSEGLLS AVLDLPQFND EERCTWYGNW RIKISDTLRK LQLQIEPELC
FLHAKGIADN AAQWLESHSG NQLYAEQWKG LWKTLENICR SAMDSSYLWK DLNRTVGKKR
AFSELLKLLE SSGLHKHKYE IMKILGDSNW LFLQPSYDAQ HLLLAPNRLS AANVSAVSEI
QCLPDGTLDT EWKAVNEFYF KSLASVQLLQ QICLKHHHDF SSEQTTRSVS FLNHLLVIQQ
MQREAAYGFA KHLKQLHKCV STFGSLYPNS TNFEEKTDNE CSFAHNQHET IKCIWQQKEL
FDSLYVMLVE ESLLLRTVER THLSDCQSVR VGAHSVLSFS KKFIPVIQKS KESLDNYLLG
PGAAITVMMG SFHHVISGQI ESLVFQNFQV INEFGEHLSA LRKEDFGRSS VIETLLSLFD
DLLKKGKRMA EQFNSALETR SYSTYSCEEA KYCNGNSSDL EAQFGRAITR IYENIMDMLQ
KLGSLSSDHA LSEESLRRVT SWEYIYKSTI AILNFDHLNY QTLEAISCAE KLVNYHGQGT
PRLSSNIEAH LKHLCKLLDL VLNFSDGFLQ DFLAMHKTTS VMIHALASIL ASLFSKGFGI
SAKDQEDDAS HDLSQDGSGT GMGEGAGVKD VSDQIDDEDQ LLGTSEKQAG EEQDASDKVP
SKDDKGIEVE QDFAADTYSV GEDSDGEDND ENGEDEQLES AMGETGANSE VVNEKLWDKE
EEENHSSAKE KYESGPSVRD KDESSRELRA KEDFVSMADE QGELDSDVTD GQKDETGDLE
ELGDAENTED LSMDKEEAFT DPTGLKLDES NENLEEDTNM DEIDGTDTKE ELGPEEPDES
AENGNHEEMD KNSADEIMEE ADGEQAGGTS EKDDANGDAE ENTEMNLTTP RKDVFKAGMS
ESTDGHVPNA ESAPQPNVGC GASKSVAPEA DWFDGNDIHN EITPLMSLPS NNTSQMDIRV
SGSSASGKPT DDIPKSQVPH QKASPVQKTN ANPYRNIGDA LEEWKERVNV SVDLRADNTE
MQGEVEDENA DEYGYVSEFD KGTAQALGPA TSEQIDKGGD TSKPNADNLA EHKNDVTEME
IEKQNSEAQP IEHRAAIIKN KMEQTPISDL EELPVQESPE THRDNDGDPG SLSESLVSIK
KSYLSEELNQ LSKLSVSENE PGKALELAEV SDDLKNNANA LWRRYEFQTA RLSQELAEQL
RLVMEPTLAS KLQGDYKTGK RINMKKVIPY IASHYRKDKI WLRRTRPNKR DYQVVIAVDD
SRSMSESGCG DVAIEALVTV CGAMSQLEMG NLSVVSFGKK GNIRSLHDFD EPFTGVAGIK
MMSGLTFEQE NTIADEPVLD LLMFLNNMLD TAVAKARLPS GQNPLQQLVL NNRRWPIPRE
VNMGGWMKFH SQAENLKRWV RDLLSKKRMV AFLLVDSPEE SIVDLKELSF EGKEIKVSKY
LDSFPFPYYI VLRNIEALPR TLADLLRQWF ELMQYTRE
//
