ID A0A2H5NNY2_CITUN Unreviewed; 900 AA.
AC A0A2H5NNY2;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=E3 ubiquitin protein ligase {ECO:0000256|RuleBase:RU365038};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU365038};
GN ORFNames=CUMW_062690 {ECO:0000313|EMBL:GAY41870.1};
OS Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=55188 {ECO:0000313|EMBL:GAY41870.1};
RN [1] {ECO:0000313|EMBL:GAY41870.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=29259619; DOI=10.3389/fgene.2017.00180;
RA Shimizu T., Tanizawa Y., Mochizuki T., Nagasaki H., Yoshioka T., Toyoda A.,
RA Fujiyama A., Kaminuma E., Nakamura Y.;
RT "Draft sequencing of the heterozygous diploid genome of Satsuma (Citrus
RT unshiu Marc.) using a hybrid assembly approach.";
RL Front. Genet. 8:180-180(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|RuleBase:RU365038};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU365038}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU365038}.
CC -!- SIMILARITY: Belongs to the BRE1 family. {ECO:0000256|ARBA:ARBA00005555,
CC ECO:0000256|RuleBase:RU365038}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAY41870.1}.
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DR EMBL; BDQV01000015; GAY41870.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2H5NNY2; -.
DR UniPathway; UPA00143; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR CDD; cd16499; RING-HC_Bre1-like; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR013956; E3_ubiquit_lig_Bre1.
DR InterPro; IPR018957; Znf_C3HC4_RING-type.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR23163:SF0; E3 UBIQUITIN-PROTEIN LIGASE BRE1; 1.
DR PANTHER; PTHR23163; RING FINGER PROTEIN-RELATED; 1.
DR Pfam; PF00097; zf-C3HC4; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Chromatin regulator {ECO:0000256|ARBA:ARBA00022853,
KW ECO:0000256|RuleBase:RU365038};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|RuleBase:RU365038};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365038}; Nucleus {ECO:0000256|RuleBase:RU365038};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|RuleBase:RU365038};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU365038};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU365038};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..900
FT /note="E3 ubiquitin protein ligase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5014160713"
FT DOMAIN 848..886
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 161..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 70..97
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 264..294
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 609..657
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 728..762
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 791..852
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 900 AA; 103089 MW; E722E8C94DB4F3F0 CRC64;
MFLLGFGLGF RVWCVFLCEW VVSMGSTGEP DRKRRHFSSI SPTAATAKKN PFFPSSEEKK
IDTAVLQFQN QKLVQKLETQ KVEYSALENK FAQLKERQQP YDSTLKVVNK SWEELITDLE
SCSMRARESS NGQESRCLSI IEDVTPHPSH DAFLSRLMET GATESSSADN CPNQMEEDRE
TGIPRTKNIV SNILAAVDNL WHLKDGLYAA VLKDLQDGGS KQKASSNLQS EVKNLRLALM
DLHLKHKSLT RELQSRQDID AKEKAKLNRL KGELESAVKE LEECNCKLAA LRAERDVTKG
AFFPVLNLGN KHVAGDRVRD EQRDLRDMES VHKELMDQAS HQLLELKGLH DGRIKVLQQL
YNLQNTLKSV KCLSSSKAFL SVKNQLEKSK SEVFKYQALF EKLQVEKDNL AWRETELNMK
IDLVDVFRRS SAVTDSKIAD LGIEIQKQID EKNRIEMRLE EASREPGRKE IIAEFRALVS
SFPEDMSAMQ RQLSKYKEAA LDIHILRADV LSLTNVLERK VKECETLLAS SADQVAEIHK
LQAMVQDLTD SNLELKLILD MYRRESTDSR DVLAARDLEY KAWAHVHSLK SSLDEQSLEL
RVKTAIEAEA ISQQRLAAAE AEIADMRQKL EAFKRDMVSL SDALKSKNEE IEAYLSEIET
IGQSYDDMQT QNQQLLQQIT ERDDYNIKLV LEGVRARQLQ DALLMDKHMM ESEIQQANAS
LNFFDMKAAR IENQLRFCLD QAQRLAEDRS QNSANLENTQ KRLSDVRKSS VQVRGSLEES
QSKVYKSRLT LMELQIELVK ERFAKKRLEE DLEMGRRKVL RLQAQTEGSS IIEELQQELR
EYREILKCSI CLERPKEVVI TKCYHLFCNP CVQKVTESRH RKCPGCAASF SPNDVKPVYI
//
