ID A0A2H5NWK6_CITUN Unreviewed; 1206 AA.
AC A0A2H5NWK6;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Purple acid phosphatase {ECO:0000256|RuleBase:RU361203};
DE EC=3.1.3.2 {ECO:0000256|RuleBase:RU361203};
GN ORFNames=CUMW_080420 {ECO:0000313|EMBL:GAY44205.1};
OS Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=55188 {ECO:0000313|EMBL:GAY44205.1, ECO:0000313|Proteomes:UP000236630};
RN [1] {ECO:0000313|EMBL:GAY44205.1, ECO:0000313|Proteomes:UP000236630}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Miyagawa wase {ECO:0000313|Proteomes:UP000236630};
RX PubMed=29259619; DOI=10.3389/fgene.2017.00180;
RA Shimizu T., Tanizawa Y., Mochizuki T., Nagasaki H., Yoshioka T., Toyoda A.,
RA Fujiyama A., Kaminuma E., Nakamura Y.;
RT "Draft sequencing of the heterozygous diploid genome of Satsuma (Citrus
RT unshiu Marc.) using a hybrid assembly approach.";
RL Front. Genet. 8:180-180(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2;
CC Evidence={ECO:0000256|RuleBase:RU361203};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|ARBA:ARBA00001962};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the metallophosphoesterase superfamily. Purple
CC acid phosphatase family. {ECO:0000256|ARBA:ARBA00008723,
CC ECO:0000256|RuleBase:RU361203}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAY44205.1}.
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DR EMBL; BDQV01000022; GAY44205.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2H5NWK6; -.
DR STRING; 55188.A0A2H5NWK6; -.
DR Proteomes; UP000236630; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0003993; F:acid phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd00839; MPP_PAPs; 2.
DR Gene3D; 3.60.21.10; -; 2.
DR Gene3D; 2.60.40.380; Purple acid phosphatase-like, N-terminal; 2.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR040974; Fn3_PAP.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR041792; MPP_PAP.
DR InterPro; IPR008963; Purple_acid_Pase-like_N.
DR InterPro; IPR015914; Purple_acid_Pase_N.
DR InterPro; IPR025733; Purple_acid_PPase_C_dom.
DR PANTHER; PTHR45778:SF16; INACTIVE PURPLE ACID PHOSPHATASE 1-RELATED; 1.
DR PANTHER; PTHR45778; PURPLE ACID PHOSPHATASE-RELATED; 1.
DR Pfam; PF17808; fn3_PAP; 2.
DR Pfam; PF00149; Metallophos; 2.
DR Pfam; PF14008; Metallophos_C; 2.
DR Pfam; PF16656; Pur_ac_phosph_N; 2.
DR SUPFAM; SSF56300; Metallo-dependent phosphatases; 2.
DR SUPFAM; SSF49363; Purple acid phosphatase, N-terminal domain; 2.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|RuleBase:RU361203};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Reference proteome {ECO:0000313|Proteomes:UP000236630};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU361203};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|RuleBase:RU361203"
FT CHAIN 24..1206
FT /note="Purple acid phosphatase"
FT /evidence="ECO:0000256|RuleBase:RU361203"
FT /id="PRO_5013987917"
FT DOMAIN 47..165
FT /note="Purple acid phosphatase Fn3-like"
FT /evidence="ECO:0000259|Pfam:PF17808"
FT DOMAIN 197..291
FT /note="Purple acid phosphatase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16656"
FT DOMAIN 304..516
FT /note="Calcineurin-like phosphoesterase"
FT /evidence="ECO:0000259|Pfam:PF00149"
FT DOMAIN 541..600
FT /note="Iron/zinc purple acid phosphatase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14008"
FT DOMAIN 699..774
FT /note="Purple acid phosphatase Fn3-like"
FT /evidence="ECO:0000259|Pfam:PF17808"
FT DOMAIN 780..882
FT /note="Purple acid phosphatase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16656"
FT DOMAIN 894..1097
FT /note="Calcineurin-like phosphoesterase"
FT /evidence="ECO:0000259|Pfam:PF00149"
FT DOMAIN 1122..1181
FT /note="Iron/zinc purple acid phosphatase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14008"
SQ SEQUENCE 1206 AA; 135562 MW; 8EC1CCECD744B6C8 CRC64;
MRELRSIFLG ILLVLGAFRS TISHEDQPLS KIAIHKAVFA LDDNAYIKAS PSILGMKGQN
SDWLTVEYNS PNPSVDDWIG VFSPSNFSSS TCPAENPRVY PPLLCSAPIK FQYANYSSPQ
YKSTGKGSLK LQLINQRSDF SFVLFTNGLL NPKVVAVSNK VTFTNPNAPV YPRLAQGKVW
NEVKTFVADN TFQLHIQMTV TWTSGYGINE AEPFVEWGPK GGDRTYSPAG TLTFGRGSMC
GAPARTVGWR DPGYIHTGFL RELWPNAMYT YKLGHRLFNG TYIWSSEYQF KASPYPGQDS
LQQVIIFGDM GKDEADGSNE YNNFQRGSLN TTRQLIQDLK KIDIVFHIGD ICYANGYISQ
WDQFTAQIEP IASTVPYMIA SGNHERDWPG TGSFYGNMDS GGECGVLVEN MFYVPTENRA
KYSTDYGMFR FCIADTEQDW REGTEQYRFI EHCLASVDRQ KQPWLIFLAH RVLGYSSDLS
YAVEGSFAEP MGRESLQKLW QKYKVDIAVF GHVHNYERIC PIYQNICTNK EKHYYKGSLN
GTIHIAAGGA GASLSPFTTL QTTWSLYRDY DHGFVKLTAL DHSNLLFEYK KSRDGKVYDS
FRISRDYRDI LACSVDSCPS MTLASYQLIL VRMRELRLIF FGILLVLGTF QVASSHGGHP
LSRIAIRKAT YALNDNAYVK ASPAVVGLKG QNSEWVTVDA STCSAENPSV NPPLLCSAPI
KYQYANYSSP QYKGTGKGSL KLMLINQRSD FSVALFSGGL PKLVAVSNKI AFTNPNAPVY
PRLAQGKTWN EMTVTWTSGY GINEAEAFVQ WGRKGGDRTH SPAGTLTFDR GSAPARTVGW
RDPGYIHTSF LKELWPNAMY TYKVGHRLFN STYIWSSEYQ FKASPYPGQN SLQRVVIFGD
MGKDEADGSN EYNDFQYASL NTTRQLIQDL KNIDIVFHIG DICYANGYIS QWDQFTAQIE
PIASTVPYMI ASGNHERDWP GTGSFYGNKD SGGECGVLAE TMYSTDYGMF RFCVADTEHD
WREGTEQYKF IEHCLASVDR QKQPWLIFLA HRVLGYSSGI FYAVDGSFAE PMGRESLQKL
WQKYKVDIAI YGHVHNYERT CPIYQNICTN KEKNYYKGTL NGTIHVVAGG GGAGLAEFTP
LQTTWSLYRD YDYGFVKLTA FDHSNLLFEY KKSSDGKVYD SFRISRDYRD ILACTVGSCP
STTLAS
//
