ID   A0A2H9VTA2_9SPHI        Unreviewed;       516 AA.
AC   A0A2H9VTA2;
DT   28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT   28-FEB-2018, sequence version 1.
DT   24-JAN-2024, entry version 10.
DE   SubName: Full=4-hydroxy 2-oxovalerate aldolase {ECO:0000313|EMBL:PJJ84055.1};
GN   ORFNames=CLV57_1059 {ECO:0000313|EMBL:PJJ84055.1};
OS   Mucilaginibacter auburnensis.
OC   Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC   Sphingobacteriaceae; Mucilaginibacter.
OX   NCBI_TaxID=1457233 {ECO:0000313|EMBL:PJJ84055.1, ECO:0000313|Proteomes:UP000242687};
RN   [1] {ECO:0000313|EMBL:PJJ84055.1, ECO:0000313|Proteomes:UP000242687}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 28175 {ECO:0000313|EMBL:PJJ84055.1,
RC   ECO:0000313|Proteomes:UP000242687};
RA   Goeker M.;
RT   "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT   (KMG-II): From Individual Species to Whole Genera.";
RL   Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+);
CC         Xref=Rhea:RHEA:19045, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30934, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.21;
CC         Evidence={ECO:0000256|ARBA:ARBA00034270};
CC   -!- PATHWAY: Amino-acid biosynthesis. {ECO:0000256|ARBA:ARBA00029440}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PJJ84055.1}.
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DR   EMBL; PGFJ01000001; PJJ84055.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2H9VTA2; -.
DR   OrthoDB; 9804858at2; -.
DR   Proteomes; UP000242687; Unassembled WGS sequence.
DR   GO; GO:0046912; F:acyltransferase activity, acyl groups converted into alkyl on transfer; IEA:InterPro.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR   CDD; cd07944; DRE_TIM_HOA_like; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR005675; Citramal_synthase.
DR   InterPro; IPR000891; PYR_CT.
DR   PANTHER; PTHR43538:SF1; (R)-CITRAMALATE SYNTHASE; 1.
DR   PANTHER; PTHR43538; ALPHA-IPM SYNTHASE/HOMOCITRATE SYNTHASE; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000242687}.
FT   DOMAIN          2..254
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000259|PROSITE:PS50991"
SQ   SEQUENCE   516 AA;  57417 MW;  CDE3F77BB99BE6E8 CRC64;
     MYKILDCTLR DGGYYNGWDF DDKLVETYLK AMEALPVSYI EVGYRNNGRY GYEGKYFYCP
     KFILEKIRSL VPSKKIAVMF NEKDVKPEEV ARLLEPCKGL VDIVRMAVAP TRIPNAIELS
     KAIKAEGFEA AVNMMYMSSL LDDKADAIFE QLPAMEGIVD YFSLVDSYGG VYPADVSRLV
     KKCKALLSMP IGFHGHNNIE LVFANSLAAI EAGCDMVDAT VTGMGRGAGN LKTELILTHQ
     ATKDKVDVDF NSLANVVTDF EELQAQYGWG TNLPYMVSGA NSLPQKDVMD WVSKRSYSIN
     SIIRGLSNQS KGQKDNLELP KFDGSKFKYK NAVIIGGGAG AIEHSEGLIR FIRQQEDICV
     IHASSKNSWA YADITHPQFF CLVGNEGHRL EKTFKDLDKV SAVCVLPPFP RKMGTYIPDA
     VIEHSFELPT VSFTNRNQDS HTALALQAVI DMGVENIYIA GYDGYAKVSI TSNEQALFNE
     NEYSFAKFAE SGKKVYSLTP TLYSLDQVSI YSLIEE
//