ID A0A2H9ZXK8_9ASPA Unreviewed; 554 AA.
AC A0A2H9ZXK8;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=Signal peptide peptidase-like 5 {ECO:0000313|EMBL:PKA48045.1};
DE EC=3.4.23.- {ECO:0000313|EMBL:PKA48045.1};
GN Name=SPPL5 {ECO:0000313|EMBL:PKA48045.1};
GN ORFNames=AXF42_Ash015808 {ECO:0000313|EMBL:PKA48045.1};
OS Apostasia shenzhenica.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC Apostasioideae; Apostasia.
OX NCBI_TaxID=1088818 {ECO:0000313|EMBL:PKA48045.1, ECO:0000313|Proteomes:UP000236161};
RN [1] {ECO:0000313|EMBL:PKA48045.1, ECO:0000313|Proteomes:UP000236161}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Shenzhen {ECO:0000313|Proteomes:UP000236161};
RC TISSUE=Stem {ECO:0000313|EMBL:PKA48045.1};
RX PubMed=28902843; DOI=10.1038/nature23897;
RA Zhang G.Q., Liu K.W., Li Z., Lohaus R., Hsiao Y.Y., Niu S.C., Wang J.Y.,
RA Lin Y.C., Xu Q., Chen L.J., Yoshida K., Fujiwara S., Wang Z.W., Zhang Y.Q.,
RA Mitsuda N., Wang M., Liu G.H., Pecoraro L., Huang H.X., Xiao X.J., Lin M.,
RA Wu X.Y., Wu W.L., Chen Y.Y., Chang S.B., Sakamoto S., Ohme-Takagi M.,
RA Yagi M., Zeng S.J., Shen C.Y., Yeh C.M., Luo Y.B., Tsai W.C.,
RA Van de Peer Y., Liu Z.J.;
RT "The Apostasia genome and the evolution of orchids.";
RL Nature 549:379-383(2017).
CC -!- FUNCTION: Intramembrane-cleaving aspartic protease (I-CLiP) that
CC cleaves type II membrane signal peptides in the hydrophobic plane of
CC the membrane. {ECO:0000256|ARBA:ARBA00003012}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane
CC {ECO:0000256|ARBA:ARBA00004337}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004337}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the peptidase A22B family.
CC {ECO:0000256|ARBA:ARBA00006859}.
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DR EMBL; KZ452995; PKA48045.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2H9ZXK8; -.
DR STRING; 1088818.A0A2H9ZXK8; -.
DR OrthoDB; 51438at2759; -.
DR Proteomes; UP000236161; Unassembled WGS sequence.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042500; F:aspartic endopeptidase activity, intramembrane cleaving; IEA:InterPro.
DR Gene3D; 3.50.30.30; -; 1.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR007369; Peptidase_A22B_SPP.
DR InterPro; IPR006639; Preselin/SPP.
DR PANTHER; PTHR12174:SF75; INTRAMEMBRANE PROTEASE (IMPAS) FAMILY; 1.
DR PANTHER; PTHR12174; SIGNAL PEPTIDE PEPTIDASE; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF04258; Peptidase_A22B; 1.
DR SMART; SM00730; PSN; 1.
DR SUPFAM; SSF52025; PA domain; 1.
PE 3: Inferred from homology;
KW Endosome {ECO:0000256|ARBA:ARBA00022753};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:PKA48045.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000236161};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..554
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5014169224"
FT TRANSMEM 192..211
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 242..265
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 271..300
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 312..335
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 341..362
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 369..392
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 424..446
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 458..479
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 491..516
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 82..165
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
SQ SEQUENCE 554 AA; 60829 MW; 7FF64C8C52E66661 CRC64;
MRTSGVFGLI TMAVVLPALV SGGDIVHEDD RFPKIPGCSN HFVLVKVQTW INGVEDSEFV
GVGARFGPTI VSKEKHANRT GLVVSDPHDC CSPPRNEISG DVLLVHRGSC KFTTKAKIAE
AAGASAILIV NNRRELYKMV CDGNETDLHI KIPAVMLPHD AGASLESVIS SKHSVAVQLY
SPDRPLVDTA EIFLWLMAVG TILCASIWSA WSSREASIEY EKLLKDAPEE LLNMESTGSS
GVVDINTLSA ILFVLIASGF LVMLYKFMST WFVEILVVLF CIGGIEGLQT CLVALLSRWF
KQAGDSFIKV PFFGAVSYLT LAITPFCVAF AVLWAVYRQK AFAWIGQDIL GIALILTVIQ
IVRVPNLKVG TVLLSCAFLY DIFWVFASHW FFHESVMIVV ARGDRSGEDG VPMLLKIPRM
FDPWGGYSII GFGDILLPGL LVAFSLRYDW ATKKNVNAGY FLWSMVAYGS GLLITYVALN
LMDGHGQPAL LYIVPFTLDI IVNGISGQII FLFFIFKTSA GTLLSLGWKR GELKNLWTTG
QPDRICPHIK ASLQ
//
