ID A0A2I0A2B6_9ASPA Unreviewed; 552 AA.
AC A0A2I0A2B6;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE SubName: Full=Signal peptide peptidase-like 2 {ECO:0000313|EMBL:PKA49663.1};
DE EC=3.4.23.- {ECO:0000313|EMBL:PKA49663.1};
GN Name=SPPL2 {ECO:0000313|EMBL:PKA49663.1};
GN ORFNames=AXF42_Ash004204 {ECO:0000313|EMBL:PKA49663.1};
OS Apostasia shenzhenica.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC Apostasioideae; Apostasia.
OX NCBI_TaxID=1088818 {ECO:0000313|EMBL:PKA49663.1, ECO:0000313|Proteomes:UP000236161};
RN [1] {ECO:0000313|EMBL:PKA49663.1, ECO:0000313|Proteomes:UP000236161}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Shenzhen {ECO:0000313|Proteomes:UP000236161};
RC TISSUE=Stem {ECO:0000313|EMBL:PKA49663.1};
RX PubMed=28902843; DOI=10.1038/nature23897;
RA Zhang G.Q., Liu K.W., Li Z., Lohaus R., Hsiao Y.Y., Niu S.C., Wang J.Y.,
RA Lin Y.C., Xu Q., Chen L.J., Yoshida K., Fujiwara S., Wang Z.W., Zhang Y.Q.,
RA Mitsuda N., Wang M., Liu G.H., Pecoraro L., Huang H.X., Xiao X.J., Lin M.,
RA Wu X.Y., Wu W.L., Chen Y.Y., Chang S.B., Sakamoto S., Ohme-Takagi M.,
RA Yagi M., Zeng S.J., Shen C.Y., Yeh C.M., Luo Y.B., Tsai W.C.,
RA Van de Peer Y., Liu Z.J.;
RT "The Apostasia genome and the evolution of orchids.";
RL Nature 549:379-383(2017).
CC -!- FUNCTION: Intramembrane-cleaving aspartic protease (I-CLiP) that
CC cleaves type II membrane signal peptides in the hydrophobic plane of
CC the membrane. {ECO:0000256|ARBA:ARBA00003012}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane
CC {ECO:0000256|ARBA:ARBA00004337}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004337}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the peptidase A22B family.
CC {ECO:0000256|ARBA:ARBA00006859}.
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DR EMBL; KZ452037; PKA49663.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2I0A2B6; -.
DR OrthoDB; 51438at2759; -.
DR Proteomes; UP000236161; Unassembled WGS sequence.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042500; F:aspartic endopeptidase activity, intramembrane cleaving; IEA:InterPro.
DR Gene3D; 3.50.30.30; -; 1.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR007369; Peptidase_A22B_SPP.
DR InterPro; IPR006639; Preselin/SPP.
DR PANTHER; PTHR12174; SIGNAL PEPTIDE PEPTIDASE; 1.
DR PANTHER; PTHR12174:SF90; SIGNAL PEPTIDE PEPTIDASE-LIKE 3; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF04258; Peptidase_A22B; 1.
DR SMART; SM00730; PSN; 1.
DR SUPFAM; SSF52025; PA domain; 1.
PE 3: Inferred from homology;
KW Endosome {ECO:0000256|ARBA:ARBA00022753};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:PKA49663.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000236161};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..38
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 39..552
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5014116561"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 269..287
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 293..319
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 331..355
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 388..411
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 447..466
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 478..500
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 506..525
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 114..182
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
SQ SEQUENCE 552 AA; 60924 MW; 907854D1F5D3E79D CRC64;
MTSPGGPVHP LRPSLLFRLV IFLVTCFVGL RNAPFAAASD DVSHDDEFAH KSEACNKEFQ
LVKVRNWVDG DESMDIVGLS ARFGAFLPRH ASEAHQWPAT AANPLNCCAN LTSKLGNSIA
IAKRGDCTFT TKAVVAQTGG ATGLLVINDS EDLYKMVCSE NDTSLNLTIP VVMIPKSAGD
KLTKLLNDGR KVVVLLYSPR RPVVDFSATF LWLMAVGTIV CASLWAEFIA CDQVDERYNQ
LTLKQDQPGT EMSSREHEKE VLEINSRGAI IFVIAASAFL MLLFYFMSSW FVLLLVVLFC
IGGVEGMHIC LVSLILRVFK YCGQKTVRLP FFGEVLVLSV VMLPLCAAFA IFWAANRHAS
YAWIGQDILG ICLMVTVLQM ARLPNIKVAS ALLCCAFVYD IFWVFVSPLI FHESVMIAVA
RGDNSGEAIP MLLRTPRFFD PWGGYDMIGF GDILFPGLLI AFSYRYDRAN KKSIVSGYFV
WLTIGYAFGL FLTYLALFLM DGHGQPALLY LVPCTLGLII ILGWVRGELG DLWNYGKIRS
SDNAPITETG EA
//
