ID A0A2I0A5M2_9ASPA Unreviewed; 578 AA.
AC A0A2I0A5M2;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Leucine aminopeptidase 2, chloroplastic {ECO:0000313|EMBL:PKA50841.1};
DE EC=3.4.11.1 {ECO:0000313|EMBL:PKA50841.1};
GN ORFNames=AXF42_Ash007496 {ECO:0000313|EMBL:PKA50841.1};
OS Apostasia shenzhenica.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC Apostasioideae; Apostasia.
OX NCBI_TaxID=1088818 {ECO:0000313|EMBL:PKA50841.1, ECO:0000313|Proteomes:UP000236161};
RN [1] {ECO:0000313|EMBL:PKA50841.1, ECO:0000313|Proteomes:UP000236161}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Shenzhen {ECO:0000313|Proteomes:UP000236161};
RC TISSUE=Stem {ECO:0000313|EMBL:PKA50841.1};
RX PubMed=28902843; DOI=10.1038/nature23897;
RA Zhang G.Q., Liu K.W., Li Z., Lohaus R., Hsiao Y.Y., Niu S.C., Wang J.Y.,
RA Lin Y.C., Xu Q., Chen L.J., Yoshida K., Fujiwara S., Wang Z.W., Zhang Y.Q.,
RA Mitsuda N., Wang M., Liu G.H., Pecoraro L., Huang H.X., Xiao X.J., Lin M.,
RA Wu X.Y., Wu W.L., Chen Y.Y., Chang S.B., Sakamoto S., Ohme-Takagi M.,
RA Yagi M., Zeng S.J., Shen C.Y., Yeh C.M., Luo Y.B., Tsai W.C.,
RA Van de Peer Y., Liu Z.J.;
RT "The Apostasia genome and the evolution of orchids.";
RL Nature 549:379-383(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
CC Evidence={ECO:0000256|ARBA:ARBA00001585};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa
CC is preferably Leu, but may be other amino acids including Pro
CC although not Arg or Lys, and Yaa may be Pro. Amino acid amides and
CC methyl esters are also readily hydrolyzed, but rates on arylamides
CC are exceedingly low.; EC=3.4.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000135};
CC -!- SUBUNIT: Homohexamer (dimer of homotrimers).
CC {ECO:0000256|ARBA:ARBA00011867}.
CC -!- SIMILARITY: Belongs to the peptidase M17 family.
CC {ECO:0000256|ARBA:ARBA00009528}.
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DR EMBL; KZ452015; PKA50841.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2I0A5M2; -.
DR STRING; 1088818.A0A2I0A5M2; -.
DR OrthoDB; 2899215at2759; -.
DR Proteomes; UP000236161; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0070006; F:metalloaminopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00433; Peptidase_M17; 1.
DR Gene3D; 3.40.220.10; Leucine Aminopeptidase, subunit E, domain 1; 1.
DR Gene3D; 3.40.630.10; Zn peptidases; 1.
DR HAMAP; MF_00181; Cytosol_peptidase_M17; 1.
DR InterPro; IPR011356; Leucine_aapep/pepB.
DR InterPro; IPR043472; Macro_dom-like.
DR InterPro; IPR000819; Peptidase_M17_C.
DR InterPro; IPR023042; Peptidase_M17_leu_NH2_pept.
DR InterPro; IPR008283; Peptidase_M17_N.
DR PANTHER; PTHR11963; LEUCINE AMINOPEPTIDASE-RELATED; 1.
DR PANTHER; PTHR11963:SF23; ZGC:152830; 1.
DR Pfam; PF00883; Peptidase_M17; 1.
DR Pfam; PF02789; Peptidase_M17_N; 1.
DR PRINTS; PR00481; LAMNOPPTDASE.
DR SUPFAM; SSF52949; Macro domain-like; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
DR PROSITE; PS00631; CYTOSOL_AP; 1.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000256|ARBA:ARBA00022438,
KW ECO:0000313|EMBL:PKA50841.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:PKA50841.1};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000236161}.
FT DOMAIN 430..437
FT /note="Cytosol aminopeptidase"
FT /evidence="ECO:0000259|PROSITE:PS00631"
SQ SEQUENCE 578 AA; 60898 MW; E53EFD8171C3B8E5 CRC64;
MTAAAAVAAA ASFASCVCWS SRFDSALFFT WQLRRPSLSF PCRLRLVLRQ SMASSLTTKA
TLGLTKPAAV EFPQISFSAK EIDISEWNGD VLAVAISEKD MSKGSDSKFE NPILKKLDEQ
LGGLLADVSE EEDFKGKAGQ AAVIRLPGLG FKRLSLVGLG PIAPSSMTTS YRGIGEAVAS
TARAAQASNA AIVVASLISK DARVLAASAI ASGTILGIYE DNRFKSETKK ALLKSVDIIG
LGAGPELEKK LRYASDISSG VIFGKELVNA PANVLTPGAL AEEVSKIASS YSDVFTAKIF
DAEQCKEMNM GSYLAVAAAS SNPPHFIHLC YRPIDGNIKT KLAIVGKGLT FDSGGYNIKT
GPGSSIELMK FDMGGSAAAF GAAKAIGQIK PPGVEVHFIV AACENMISGT GMRPGDIITA
SNGKTIEVNN TDAEGRLTLA DALVYACNQG IEKVVDLATL TGACIVALGP SIAGFFTPSD
ELAKEIIAAS EVAGEKLWRM PLEDSYWESM KSSVADMLNT GGRPGGAITA ALFLKQFVDE
KVQWMHIDMA GPVWNEKKRT STGFGVSTLV EWVLLNSS
//
