ID A0A2I0AEP7_9ASPA Unreviewed; 648 AA.
AC A0A2I0AEP7;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Protein HOTHEAD {ECO:0000313|EMBL:PKA53985.1};
DE EC=1.1.-.- {ECO:0000313|EMBL:PKA53985.1};
GN Name=HTH {ECO:0000313|EMBL:PKA53985.1};
GN ORFNames=AXF42_Ash016150 {ECO:0000313|EMBL:PKA53985.1};
OS Apostasia shenzhenica.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC Apostasioideae; Apostasia.
OX NCBI_TaxID=1088818 {ECO:0000313|EMBL:PKA53985.1, ECO:0000313|Proteomes:UP000236161};
RN [1] {ECO:0000313|EMBL:PKA53985.1, ECO:0000313|Proteomes:UP000236161}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Shenzhen {ECO:0000313|Proteomes:UP000236161};
RC TISSUE=Stem {ECO:0000313|EMBL:PKA53985.1};
RX PubMed=28902843; DOI=10.1038/nature23897;
RA Zhang G.Q., Liu K.W., Li Z., Lohaus R., Hsiao Y.Y., Niu S.C., Wang J.Y.,
RA Lin Y.C., Xu Q., Chen L.J., Yoshida K., Fujiwara S., Wang Z.W., Zhang Y.Q.,
RA Mitsuda N., Wang M., Liu G.H., Pecoraro L., Huang H.X., Xiao X.J., Lin M.,
RA Wu X.Y., Wu W.L., Chen Y.Y., Chang S.B., Sakamoto S., Ohme-Takagi M.,
RA Yagi M., Zeng S.J., Shen C.Y., Yeh C.M., Luo Y.B., Tsai W.C.,
RA Van de Peer Y., Liu Z.J.;
RT "The Apostasia genome and the evolution of orchids.";
RL Nature 549:379-383(2017).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|PIRSR:PIRSR000137-2};
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC {ECO:0000256|ARBA:ARBA00010790}.
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DR EMBL; KZ451987; PKA53985.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2I0AEP7; -.
DR STRING; 1088818.A0A2I0AEP7; -.
DR OrthoDB; 52047at2759; -.
DR Proteomes; UP000236161; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0016614; F:oxidoreductase activity, acting on CH-OH group of donors; IEA:InterPro.
DR Gene3D; 3.30.410.40; -; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR012132; GMC_OxRdtase.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR PANTHER; PTHR45968:SF3; OS04G0573100 PROTEIN; 1.
DR PANTHER; PTHR45968; OSJNBA0019K04.7 PROTEIN; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS00624; GMC_OXRED_2; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR000137-3};
KW FAD {ECO:0000256|PIRSR:PIRSR000137-2};
KW Flavoprotein {ECO:0000256|PIRSR:PIRSR000137-2};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Oxidoreductase {ECO:0000313|EMBL:PKA53985.1};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000236161};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 36..53
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 65..84
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 338..352
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS00624"
FT BINDING 177
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 582..583
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 611
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 622..623
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT DISULFID 519..574
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-3"
SQ SEQUENCE 648 AA; 71018 MW; 3DCFF6E87A0023D0 CRC64;
MAVGCRQPPR EFGPPSLGCH RSSSPIYFLS LSPPPSIESL YFFFFFFFFF KEIDQMGIRR
LQGEWFLLLL LLLLCFVGCC HSEQAPNYTF VKQATEAPAV SYYDYIIVGG GTAGCPLAAT
LSRTSDVLLL ERGGSPYGNK NISNLAFFAD TLADVSPTSP SQRFVSEDGV FNARARVLGG
GSCLNAGFYT RAGADYIRGA GWDATLVEES YRWVEKVVAF EPPVRQWQSA VRDGLLEVGI
TPYNGFTYDH LYGTKVGGTI FDRNGYRHTA ADLLAYADPA RLTVLLRATA ERIVWRGDGK
GRRGRPAAQG VVFVDSAGRR RQAYLRKGKG SEVIVAAGAM GSPQLLMLSG VGPREGLERL
GIGVVAERDM VGKGMADNPM NAIFVPSPAP VEVSLIQVVG ITRFGTYIEA ASGQYFGSPS
NSGGDGDAAR PPAKNFGIFS PQTGQLSTVP PKQRTQEALD RAARAMARLD PAAFQGGFIL
EKIVGPVSRG HLELRTRDPH DNPSVTFNYF QDPIDLRRCV DGIKTIEKVI QSKAFDRFRY
PDISIQALAN MTARFPVNLL PRHDNDSRSP EQFCRDTVMT IWHYHGGCQV GRVVDGDYRV
LGVDALRVID GSTFNFSPGT NPQATVMMLG RYMGVKILEE RLRATEED
//
