ID   A0A2I0AGP6_9ASPA        Unreviewed;       164 AA.
AC   A0A2I0AGP6;
DT   28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT   28-FEB-2018, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   RecName: Full=Nicotianamine synthase {ECO:0000256|ARBA:ARBA00012675, ECO:0000256|RuleBase:RU368095};
DE            EC=2.5.1.43 {ECO:0000256|ARBA:ARBA00012675, ECO:0000256|RuleBase:RU368095};
GN   Name=CHLN {ECO:0000313|EMBL:PKA54666.1};
GN   ORFNames=AXF42_Ash000501 {ECO:0000313|EMBL:PKA54666.1};
OS   Apostasia shenzhenica.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Apostasioideae; Apostasia.
OX   NCBI_TaxID=1088818 {ECO:0000313|EMBL:PKA54666.1, ECO:0000313|Proteomes:UP000236161};
RN   [1] {ECO:0000313|EMBL:PKA54666.1, ECO:0000313|Proteomes:UP000236161}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Shenzhen {ECO:0000313|Proteomes:UP000236161};
RC   TISSUE=Stem {ECO:0000313|EMBL:PKA54666.1};
RX   PubMed=28902843; DOI=10.1038/nature23897;
RA   Zhang G.Q., Liu K.W., Li Z., Lohaus R., Hsiao Y.Y., Niu S.C., Wang J.Y.,
RA   Lin Y.C., Xu Q., Chen L.J., Yoshida K., Fujiwara S., Wang Z.W., Zhang Y.Q.,
RA   Mitsuda N., Wang M., Liu G.H., Pecoraro L., Huang H.X., Xiao X.J., Lin M.,
RA   Wu X.Y., Wu W.L., Chen Y.Y., Chang S.B., Sakamoto S., Ohme-Takagi M.,
RA   Yagi M., Zeng S.J., Shen C.Y., Yeh C.M., Luo Y.B., Tsai W.C.,
RA   Van de Peer Y., Liu Z.J.;
RT   "The Apostasia genome and the evolution of orchids.";
RL   Nature 549:379-383(2017).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine which serves as a
CC       sensor for the physiological iron status within the plant, and/or might
CC       be involved in the transport of iron. {ECO:0000256|RuleBase:RU368095}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC         Evidence={ECO:0000256|ARBA:ARBA00000566,
CC         ECO:0000256|RuleBase:RU368095};
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000256|ARBA:ARBA00007009, ECO:0000256|RuleBase:RU368095}.
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DR   EMBL; KZ451982; PKA54666.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2I0AGP6; -.
DR   OrthoDB; 2718460at2759; -.
DR   Proteomes; UP000236161; Unassembled WGS sequence.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; NICOTIANAMINE SYNTHASE 3; 1.
DR   PANTHER; PTHR32266:SF12; NICOTIANAMINE SYNTHASE 3; 1.
DR   Pfam; PF03059; NAS; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000236161};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|RuleBase:RU368095};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368095}.
SQ   SEQUENCE   164 AA;  17049 MW;  FF6FF5D896114E8B CRC64;
     MAAALVAPDK DLSSRMHFIT DDAGAIAAEK LSRYGVIFLA ALVGVDREQK AAVVEHLARS
     MAPGAMLVVR SAHGARMFLY PVVEPADLKG FEVLSVYHPN DEVINSVVIA RKPAPQHAGH
     GGSGGGGCGA VGAVIANCKC CEWTAMKNGH GGVMVEEMEL PSGI
//