ID A0A2I0BBU3_9ASPA Unreviewed; 460 AA.
AC A0A2I0BBU3;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE SubName: Full=Delta(8)-fatty-acid desaturase {ECO:0000313|EMBL:PKA65265.1};
DE EC=1.14.19.4 {ECO:0000313|EMBL:PKA65265.1};
GN Name=sld1 {ECO:0000313|EMBL:PKA65265.1};
GN ORFNames=AXF42_Ash005597 {ECO:0000313|EMBL:PKA65265.1};
OS Apostasia shenzhenica.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC Apostasioideae; Apostasia.
OX NCBI_TaxID=1088818 {ECO:0000313|EMBL:PKA65265.1, ECO:0000313|Proteomes:UP000236161};
RN [1] {ECO:0000313|EMBL:PKA65265.1, ECO:0000313|Proteomes:UP000236161}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Shenzhen {ECO:0000313|Proteomes:UP000236161};
RC TISSUE=Stem {ECO:0000313|EMBL:PKA65265.1};
RX PubMed=28902843; DOI=10.1038/nature23897;
RA Zhang G.Q., Liu K.W., Li Z., Lohaus R., Hsiao Y.Y., Niu S.C., Wang J.Y.,
RA Lin Y.C., Xu Q., Chen L.J., Yoshida K., Fujiwara S., Wang Z.W., Zhang Y.Q.,
RA Mitsuda N., Wang M., Liu G.H., Pecoraro L., Huang H.X., Xiao X.J., Lin M.,
RA Wu X.Y., Wu W.L., Chen Y.Y., Chang S.B., Sakamoto S., Ohme-Takagi M.,
RA Yagi M., Zeng S.J., Shen C.Y., Yeh C.M., Luo Y.B., Tsai W.C.,
RA Van de Peer Y., Liu Z.J.;
RT "The Apostasia genome and the evolution of orchids.";
RL Nature 549:379-383(2017).
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC {ECO:0000256|ARBA:ARBA00009295}.
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DR EMBL; KZ451895; PKA65265.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2I0BBU3; -.
DR STRING; 1088818.A0A2I0BBU3; -.
DR OrthoDB; 294339at2759; -.
DR Proteomes; UP000236161; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0102003; F:Delta8-sphingolipid desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR CDD; cd03506; Delta6-FADS-like; 1.
DR Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR005804; FA_desaturase_dom.
DR InterPro; IPR012171; Fatty_acid_desaturase.
DR PANTHER; PTHR19353:SF30; DELTA 8-(E)-SPHINGOLIPID DESATURASE; 1.
DR PANTHER; PTHR19353; FATTY ACID DESATURASE 2; 1.
DR Pfam; PF00173; Cyt-b5; 1.
DR Pfam; PF00487; FA_desaturase; 1.
DR PIRSF; PIRSF015921; FA_sphinglp_des; 1.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Oxidoreductase {ECO:0000313|EMBL:PKA65265.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000236161};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 124..143
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 149..171
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 183..204
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 18..93
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000259|PROSITE:PS50255"
SQ SEQUENCE 460 AA; 52515 MW; 21309E6157D23CFD CRC64;
MEEVGAISSG VALEPEKRKF ISSEELRKHN SPSDLWLSIQ GKVYDVTDWV KHHPGGEIPL
LDLAGQDATD AFVAYHPASA WKTLDRFFVG YHLSDYSVSE VSKDYRRLVA EFSKIGFFER
KGHGVCLSFC CMTALFSLAV YFIAGTASFW AHLAAGGMMG ILWNQSGFLG HDSGHYNVMI
SPWFNRLAQI VSGNCLTGIS IGWWKRNHNA HHIACNSLDY DPDLQHIPLF AVSSKFFSSL
QSYFYERKMT FDSAARFLIS YQHFTFYPVL CFARLNLFAQ SVILLLSKKK VPGRWQEIVG
LIVFWIWYPY LISFLPNWGE RLMFIIASFT VTGIQHVQFC LNHFSANVYV GKPEGNDWFE
KQTKGTIDIA CSPWMDWFHG GLQFQVEHHL FPRLPRCHLR RIAPLVKDLC KKHKLQYTSV
GFFEANKRTL ATLRAAAMQA RSLENSAPKN LVWEALNTHG
//
