ID A0A2I0HHV8_PUNGR Unreviewed; 760 AA.
AC A0A2I0HHV8;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00012265};
DE EC=2.5.1.114 {ECO:0000256|ARBA:ARBA00012265};
DE AltName: Full=tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00031315};
GN ORFNames=CRG98_048405 {ECO:0000313|EMBL:PKI31203.1};
OS Punica granatum (Pomegranate).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Lythraceae; Punica.
OX NCBI_TaxID=22663 {ECO:0000313|EMBL:PKI31203.1, ECO:0000313|Proteomes:UP000233551};
RN [1] {ECO:0000313|EMBL:PKI31203.1, ECO:0000313|Proteomes:UP000233551}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. AG2017 {ECO:0000313|Proteomes:UP000233551};
RC TISSUE=Leaf {ECO:0000313|EMBL:PKI31203.1};
RA Akparov Z., Amiraslanov A., Hajiyeva S., Abbasov M., Kaur K., Hamwieh A.,
RA Solovyev V., Salamov A., Braich B., Kosarev P., Mahmoud A., Hajiyev E.,
RA Babayeva S., Izzatullayeva V., Mammadov A., Mammadov A., Sharifova S.,
RA Ojaghi J., Eynullazada K., Bayramov B., Abdulazimova A., Shahmuradov I.;
RT "De-novo sequencing of pomegranate (Punica granatum L.) genome.";
RL Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent transferase that acts as a
CC component of the wybutosine biosynthesis pathway. Wybutosine is a hyper
CC modified guanosine with a tricyclic base found at the 3'-position
CC adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes
CC the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-
CC adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14)
CC to produce wybutosine-86. {ECO:0000256|ARBA:ARBA00037786}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine =
CC 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) +
CC H(+) + S-methyl-5'-thioadenosine; Xref=Rhea:RHEA:36355, Rhea:RHEA-
CC COMP:10164, Rhea:RHEA-COMP:10378, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:64315,
CC ChEBI:CHEBI:73550; EC=2.5.1.114;
CC Evidence={ECO:0000256|ARBA:ARBA00036405};
CC -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004797}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PKI31203.1}.
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DR EMBL; PGOL01009137; PKI31203.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2I0HHV8; -.
DR STRING; 22663.A0A2I0HHV8; -.
DR UniPathway; UPA00375; -.
DR Proteomes; UP000233551; Unassembled WGS sequence.
DR GO; GO:0102522; F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.120.10.80; Kelch-type beta propeller; 2.
DR Gene3D; 3.30.300.110; Met-10+ protein-like domains; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR011498; Kelch_2.
DR InterPro; IPR030382; MeTrfase_TRM5/TYW2.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR23245; TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR23245:SF25; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 2 HOMOLOG; 1.
DR Pfam; PF07646; Kelch_2; 1.
DR Pfam; PF13418; Kelch_4; 1.
DR Pfam; PF13964; Kelch_6; 1.
DR Pfam; PF02475; Met_10; 1.
DR SMART; SM00612; Kelch; 3.
DR SUPFAM; SSF117281; Kelch motif; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51684; SAM_MT_TRM5_TYW2; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000233551};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 498..757
FT /note="SAM-dependent methyltransferase TRM5/TYW2-type"
FT /evidence="ECO:0000259|PROSITE:PS51684"
SQ SEQUENCE 760 AA; 83855 MW; B31317B12DFBD7D4 CRC64;
MPIEKLFLWG HSACVIDKGS TKKVLVHGGF GGMGRHARRN DSLLLDPLDG TVEAIDTAGA
PSPRMGHTAS LIGEQMFVIG GRADPTSILS EIWALNTATN QWTLLNCNGD SFPPRHRHAA
AVLGSRIYVF GGLNNDTIFS SIFVLDEKDL RWEELKVGGE QPCARHSHSM VAFGSQIFMF
GGYNGEKALG DLYSFNVKTR VWRKEKALGR SPYARFSHSM FVYKHCLGII GGCPVKQHFQ
ELSLLDMHSQ SWKHFSLNSV GKELFVRSTV SVVGDDLVIV GGGASCYAFG TKFSEPMNIA
LHTLAASNQF PFNCQQVQQL ENPQNLGEAL GSSSEDGKGL SETWKSASNW YLQFERKHAK
PGKDLLKKFG WLDHGRKVCS SSNGTRICFP VTEKLYILFH EKGRVDPSID TSHGSESNIV
ELPPLKESVG QGISLNEISS SKALNLLKQC GGTILAYETA EFRKSVRSPS IVIKQAAASL
IKQKGLPEKL LEQLPSRWER LGDIVVLPGT SFKDPLWDTI GGELWPIIAK ALNTNRLARQ
GCVAPTGTRD STLKMLVGDN GWVDHRENGI LYSFDATKCM FSWGNLSEKL RMARLDCGDE
IIVDLFAGIG YFTLSFLVRA KAKLVYACEW NPHAIEALHH NLKANSVADR CIVLEGDNRI
TAPKGVADRV CLGLLPSSEG SWVTAVRVLR KEGGMLHVHG NVKDSDENTW VEHVANSIHS
IATSEGRCWE VTIGHVERVK WYAPHIRHLV ADVRCKGNQR
//
