ID   A0A2I0J542_PUNGR        Unreviewed;       307 AA.
AC   A0A2I0J542;
DT   28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT   28-FEB-2018, sequence version 1.
DT   27-MAR-2024, entry version 14.
DE   RecName: Full=Malic enzyme NAD-binding domain-containing protein {ECO:0000259|SMART:SM00919};
GN   ORFNames=CRG98_028218 {ECO:0000313|EMBL:PKI51357.1};
OS   Punica granatum (Pomegranate).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Myrtales; Lythraceae; Punica.
OX   NCBI_TaxID=22663 {ECO:0000313|EMBL:PKI51357.1, ECO:0000313|Proteomes:UP000233551};
RN   [1] {ECO:0000313|EMBL:PKI51357.1, ECO:0000313|Proteomes:UP000233551}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. AG2017 {ECO:0000313|Proteomes:UP000233551};
RC   TISSUE=Leaf {ECO:0000313|EMBL:PKI51357.1};
RA   Akparov Z., Amiraslanov A., Hajiyeva S., Abbasov M., Kaur K., Hamwieh A.,
RA   Solovyev V., Salamov A., Braich B., Kosarev P., Mahmoud A., Hajiyev E.,
RA   Babayeva S., Izzatullayeva V., Mammadov A., Mammadov A., Sharifova S.,
RA   Ojaghi J., Eynullazada K., Bayramov B., Abdulazimova A., Shahmuradov I.;
RT   "De-novo sequencing of pomegranate (Punica granatum L.) genome.";
RL   Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PKI51357.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; PGOL01002010; PKI51357.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2I0J542; -.
DR   STRING; 22663.A0A2I0J542; -.
DR   Proteomes; UP000233551; Unassembled WGS sequence.
DR   GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR012302; Malic_NAD-bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR23406; MALIC ENZYME-RELATED; 1.
DR   PANTHER; PTHR23406:SF32; NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PIRSF; PIRSF000106; ME; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   SMART; SM00919; Malic_M; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000233551}.
FT   DOMAIN          12..278
FT                   /note="Malic enzyme NAD-binding"
FT                   /evidence="ECO:0000259|SMART:SM00919"
FT   BINDING         164
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000106-2"
FT   BINDING         209
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000106-2"
SQ   SEQUENCE   307 AA;  32635 MW;  4B1B2901C90F60B7 CRC64;
     MSSLLRLCGE SGTAGVAIAG LLGAVRAQGR PMIDFPKMKI VVAGAGSAGI GVLNAARKTM
     ARMLGDTETA FDSARSQFWV VDAKGLITEE RQNIDPEAQP FAWKVKEVKR QGLREGASLV
     EVVRQVKPDV LLGLSAVGGL FSKEVLEAMK SSTSTRPAIF AMSNPTKNAE CTPEEAFSIV
     GENAIFASGS PFQDVDLGNG HIGHCNQGNN MYLFPGIGLG TLLSGSRIIS DGMLQAAAEC
     LAAYMTEEEV VKGVIFPSIS SIRDITKQVA AAVVREAIEE DLAEGYREMD ARELGRLSQA
     YSLNLFC
//