ID A0A2I0NNE1_9EURY Unreviewed; 700 AA.
AC A0A2I0NNE1;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Isoleucine--tRNA ligase {ECO:0000313|EMBL:PKL55499.1};
DE Flags: Fragment;
GN ORFNames=CVV35_09670 {ECO:0000313|EMBL:PKL55499.1};
OS Methanomicrobiales archaeon HGW-Methanomicrobiales-6.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales.
OX NCBI_TaxID=2013822 {ECO:0000313|EMBL:PKL55499.1, ECO:0000313|Proteomes:UP000233331};
RN [1] {ECO:0000313|EMBL:PKL55499.1, ECO:0000313|Proteomes:UP000233331}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HGW-Methanomicrobiales-6 {ECO:0000313|EMBL:PKL55499.1};
RX PubMed=28350393; DOI=10.1038/ismej.2017.39;
RA Hernsdorf A.W., Amano Y., Miyakawa K., Ise K., Suzuki Y., Anantharaman K.,
RA Probst A., Burstein D., Thomas B.C., Banfield J.F.;
RT "Potential for microbial H2 and metal transformations associated with novel
RT bacteria and archaea in deep terrestrial subsurface sediments.";
RL ISME J. 11:1915-1929(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PKL55499.1}.
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DR EMBL; PGYH01000095; PKL55499.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2I0NNE1; -.
DR Proteomes; UP000233331; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:PKL55499.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917}.
FT DOMAIN 43..271
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 329..477
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:PKL55499.1"
SQ SEQUENCE 700 AA; 80186 MW; DF6910FBB0E0F3DC CRC64;
AFADMYVRDA NDLVIDALGG YLLARRTITH RYGHCWRCKT PIIYRATAQW FLKATEIREP
MLQEITKVKW YPEWAGSARF HDFVRDSRDW CISRQRYWGI PIPIWQCEQC GERTVIGTIA
ELEERSGAKV PDPHRPYVDE VVIPCSCGGE MRRVDDIFDV WFDSAVASWA TLRFPRKREA
FDRLWPADFI TEGQDQTRGW FYSQLGASTV AFGRAPYKSV LMHGFALDAE GRKMSKSFGN
VVTPEEVMNQ FGVDVLRFYV LWASAPWDDL KFNWDSVKTI HRTLNILWNV YRFPLPYMVL
DSFEPAFGEG NRWDDSFVQA NIYDMPEEDR WIVSRINSLA RTTAEDMQEY QLHKVTRALA
AFILEDLSRW YVQLVRPRMW LEEDSPEKRY AYETVYYVMR RLTALLAPFT PHIAEEIYGN
LRLAGDPESV HMLDWPEADD LLIAPDLESA MEVVRSFDDA VATARQNGRR KLRWPISETV
VVTGSGEVKT ALEDLNELAL NRANSRAVRV VTGRWDRILW QAEPVMRAIG PEFGKEGPKI
KALIERADGT ALKAAIERDG KAELDGYEIA GRHVTFAEAL PEGVFAAPMK DATVYVDVTL
TPALEAEGYA REVIRRIQEM RRQLDLNVDD FIVAAVDIAD ERVASLIGEE AWQKEIAGEV
RAATLTVHRN GEKPAETFAL EKDWDVEGVQ MQIGISRAGE
//
