ID   A0A2I0QV30_9BACI        Unreviewed;       352 AA.
AC   A0A2I0QV30;
DT   28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT   28-FEB-2018, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=Endoglucanase {ECO:0000313|EMBL:PKR78178.1};
GN   ORFNames=CEY16_08330 {ECO:0000313|EMBL:PKR78178.1};
OS   Halalkalibacillus sediminis.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacillus.
OX   NCBI_TaxID=2018042 {ECO:0000313|EMBL:PKR78178.1, ECO:0000313|Proteomes:UP000243524};
RN   [1] {ECO:0000313|EMBL:PKR78178.1, ECO:0000313|Proteomes:UP000243524}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B3227 {ECO:0000313|EMBL:PKR78178.1,
RC   ECO:0000313|Proteomes:UP000243524};
RA   He R.-H., Du Z.-J.;
RT   "the draft geome sequence of Illustriluteabacillus marina B3227.";
RL   Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000256|PIRSR:PIRSR001123-2};
CC   -!- SIMILARITY: Belongs to the peptidase M42 family.
CC       {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PKR78178.1}.
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DR   EMBL; PJNH01000002; PKR78178.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2I0QV30; -.
DR   OrthoDB; 9772053at2; -.
DR   Proteomes; UP000243524; Unassembled WGS sequence.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd05656; M42_Frv; 1.
DR   Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR008007; Peptidase_M42.
DR   InterPro; IPR023367; Peptidase_M42_dom2.
DR   PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR32481:SF9; ENDOGLUCANASE; 1.
DR   Pfam; PF05343; Peptidase_M42; 1.
DR   PIRSF; PIRSF001123; PepA_GA; 1.
DR   SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000243524}.
FT   ACT_SITE        207
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT   BINDING         62
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         175
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         175
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         208
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         230
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         317
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ   SEQUENCE   352 AA;  38623 MW;  4449E7CE6196676C CRC64;
     MENLLKDLTN IHGPCGFEED VATYIAKRLN DSVDSIEVDG VGNLTVRKKG RKDGPKIVVA
     AHMDEIGFIV KKIEENGLLR FEKLGGHDDR ILLSQRVQLN TSKGLRNGII GTISAHMKKF
     DEPNKIRSHQ QLYIDIGANS KQDAAELGVK VGDTVTWHPY YDRLTENRVM GKAFDDRAGC
     AVLIQAFEEL EANDFSGEMV GVFTVQEEVG LRGARVASRQ QNADVAIALD TTAVSDTPEE
     MMDQTLAIGG GTGIKVLDFS LIANKKVRNH LVEIAEREKI THQLEVFPGI GTDAGELSLA
     HQGVPTGVLS IPSRYAHSSV EVVDLNDIEA TKNLLVAFVK EMKATVEYKF NI
//