ID A0A2I0R6D9_9FLAO Unreviewed; 755 AA.
AC A0A2I0R6D9;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=NADP-dependent malic enzyme {ECO:0000313|EMBL:PKR82153.1};
DE EC=1.1.1.40 {ECO:0000313|EMBL:PKR82153.1};
GN ORFNames=CW751_02110 {ECO:0000313|EMBL:PKR82153.1};
OS Brumimicrobium salinarum.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Crocinitomicaceae; Brumimicrobium.
OX NCBI_TaxID=2058658 {ECO:0000313|EMBL:PKR82153.1, ECO:0000313|Proteomes:UP000236654};
RN [1] {ECO:0000313|EMBL:PKR82153.1, ECO:0000313|Proteomes:UP000236654}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LHR20 {ECO:0000313|EMBL:PKR82153.1,
RC ECO:0000313|Proteomes:UP000236654};
RA Do Z.-J., Luo H.-R.;
RT "The draft genome sequence of Brumimicrobium saltpan LHR20.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: In the N-terminal section; belongs to the malic enzymes
CC family. {ECO:0000256|ARBA:ARBA00007686}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PKR82153.1}.
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DR EMBL; PJNI01000001; PKR82153.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2I0R6D9; -.
DR OrthoDB; 9805787at2; -.
DR Proteomes; UP000236654; Unassembled WGS sequence.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0004473; F:malate dehydrogenase (decarboxylating) (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0008948; F:oxaloacetate decarboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10950; -; 1.
DR Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR012188; ME_PTA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR042113; P_AcTrfase_dom1.
DR InterPro; IPR042112; P_AcTrfase_dom2.
DR InterPro; IPR002505; PTA_PTB.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR Pfam; PF01515; PTA_PTB; 1.
DR PIRSF; PIRSF036684; ME_PTA; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036684-2};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|PIRSR:PIRSR036684-3};
KW Oxidoreductase {ECO:0000313|EMBL:PKR82153.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000236654}.
FT DOMAIN 18..151
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 163..400
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
FT ACT_SITE 94
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-1"
FT BINDING 76..83
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 136
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 137
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 162
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 287
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
SQ SEQUENCE 755 AA; 82993 MW; 8399CE61D07D8C64 CRC64;
MAKIKKQDAL DYHSQGKPGK IEVIPTKPYS SQRDLSLAYS PGVAEPCLKI AENPDDVYKY
TAKSNLVAVI SNGTAVLGLG DIGPAASKPV MEGKGLLFKI FADIDVFDIE VDANDPETFI
QTVKAIAPTF GGINLEDIKA PEAFEIEDRL KEELDIPVMH DDQHGTAIIS SAGLINALEL
ADKKIEEVKI VVSGAGASAM SCAKLYNSLG AKMENIFMFD SKGLLSKDRE NLSGNKAFFA
EHHENTSDLA TELKGADVLI GLSRGGVVSK QMVKSMADNP IVFALANPEP EIAYDDAMSV
RKDVIMATGR SDHPNQVNNV LGFPFIFRGA LDVRAKSINE EMKLAAVYAI ANLAKETVPD
EVSEAYGERS ITFGRDQFIP KPLDPRLIYY VAPAVAKAAM DSGVARTPIE DWDAYDAELK
KRLGIDNKLV RTITDKAERN PKRVVFAEAD NYKILKAAQT AMEEGVATPI LLGRKEKVLH
MIEEYNMDFP DVEVVDPKDI GEKDRLERYA EAFFELRQRK GFTAYEASKI MRERNYFGAM
MVQSGDADAM ISGATRKYSD VLRPALQVIG TQKDVDRVAG MYILITKRGP IFMADTTVNF
DPTAEEIADM THHIAKTIRK FKVNPNIALL SYSNFGSAEG KDAKKMAKAV EIIHDKYPTL
RVDGEVQANF ALNNELMNEK FAFSELTNKK VNTFIFPNLS AGNIAYKLLQ EMTEAEAIGP
VLVGLRKSVH VLQLGASVRE IVNMVKVAVV DAQNK
//
