ID A0A2I3HVI4_NOMLE Unreviewed; 147 AA.
AC A0A2I3HVI4;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 D3 {ECO:0000256|ARBA:ARBA00040338};
DE EC=2.3.2.23 {ECO:0000256|ARBA:ARBA00012486};
DE EC=2.3.2.24 {ECO:0000256|ARBA:ARBA00039076};
DE AltName: Full=(E3-independent) E2 ubiquitin-conjugating enzyme D3 {ECO:0000256|ARBA:ARBA00041314};
DE AltName: Full=E2 ubiquitin-conjugating enzyme D3 {ECO:0000256|ARBA:ARBA00041427};
DE AltName: Full=Ubiquitin carrier protein D3 {ECO:0000256|ARBA:ARBA00041862};
DE AltName: Full=Ubiquitin-protein ligase D3 {ECO:0000256|ARBA:ARBA00043109};
GN Name=UBE2D3 {ECO:0000313|Ensembl:ENSNLEP00000047532.1};
OS Nomascus leucogenys (Northern white-cheeked gibbon) (Hylobates leucogenys).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hylobatidae;
OC Nomascus.
OX NCBI_TaxID=61853 {ECO:0000313|Ensembl:ENSNLEP00000047532.1, ECO:0000313|Proteomes:UP000001073};
RN [1] {ECO:0000313|Ensembl:ENSNLEP00000047532.1, ECO:0000313|Proteomes:UP000001073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Gibbon Genome Sequencing Consortium;
RL Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSNLEP00000047532.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000256|ARBA:ARBA00000485};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-
CC cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.24; Evidence={ECO:0000256|ARBA:ARBA00035845};
CC -!- PATHWAY: Protein modification. {ECO:0000256|ARBA:ARBA00043952}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000256|RuleBase:RU362109}.
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DR EMBL; ADFV01176944; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ADFV01176945; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_003257519.1; XM_003257471.2.
DR RefSeq; XP_003257520.1; XM_003257472.3.
DR RefSeq; XP_003257521.1; XM_003257473.2.
DR RefSeq; XP_003257522.1; XM_003257474.3.
DR RefSeq; XP_004089080.1; XM_004089032.2.
DR RefSeq; XP_004089081.1; XM_004089033.2.
DR RefSeq; XP_004089082.1; XM_004089034.2.
DR RefSeq; XP_012354856.1; XM_012499402.1.
DR RefSeq; XP_012354857.1; XM_012499403.1.
DR RefSeq; XP_012354858.1; XM_012499404.1.
DR AlphaFoldDB; A0A2I3HVI4; -.
DR SMR; A0A2I3HVI4; -.
DR Ensembl; ENSNLET00000034652.1; ENSNLEP00000047532.1; ENSNLEG00000035663.1.
DR GeneID; 100607763; -.
DR KEGG; nle:100607763; -.
DR CTD; 7323; -.
DR GeneTree; ENSGT00940000153169; -.
DR OrthoDB; 5478564at2759; -.
DR Proteomes; UP000001073; Chromosome 9.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0019787; F:ubiquitin-like protein transferase activity; IEA:UniProt.
DR GO; GO:0032446; P:protein modification by small protein conjugation; IEA:UniProt.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR PANTHER; PTHR24068; UBIQUITIN-CONJUGATING ENZYME E2; 1.
DR PANTHER; PTHR24068:SF415; UBIQUITIN-CONJUGATING ENZYME E2 D3; 1.
DR Pfam; PF00179; UQ_con; 1.
DR SMART; SM00212; UBCc; 1.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU362109};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU362109};
KW Reference proteome {ECO:0000313|Proteomes:UP000001073};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|RuleBase:RU362109}.
FT DOMAIN 1..147
FT /note="UBC core"
FT /evidence="ECO:0000259|PROSITE:PS50127"
FT ACT_SITE 85
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 147 AA; 16687 MW; ADD74A8A708EFEE3 CRC64;
MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY
PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV
PEIARIYKTD RDKYNRISRE WTQKYAM
//
